精巣・卵巣・前立腺・生殖腺・子宮へのエレクトロポレーションによる遺伝子導入

NEPA21 Type III エレクトロポレーション装置

NEPA21エレクトロポレーション装置

Sピンセット電極

ｹｰﾌﾞﾙ･ﾌｯﾄｽｲｯﾁ

精巣・卵巣・前立腺・生殖腺・子宮へのエレクトロポレーションによる遺伝子導入

アプリケーション

マウス精巣への遺伝子導入及び電圧変化によるGFP発現

実体顕微鏡により撮影した精巣の画像。 様々な電圧で精巣に遺伝子導入を行ない、5週間後に可視光（A）と励起光（B）で観察を行なった。

  1. 各々の精巣には、出力された電圧が表示されています。
  2. 精巣（12日目）に様々な電圧で遺伝子導入を行なってから5週間後の重量の変化を示しています。
  3. 50Ｖで遺伝子導入を行なった精巣の断面図。左： 励起光下での蛍光顕微鏡の画像、右：ヘマトキシリンによる対比染色。
  4.  別の断面のヘマトキシリンによる染色。

精巣に高電圧で遺伝子導入をおこなった場合、精細管の管腔の拡張（D）や被膜下の精細管の完全な変性（D左）が観察された。Eでは、多くの蛍光陽性なセルトリ細胞が容易に確認出来た。

A, Bのスケールバー：2mm、D, Eのスケールバー：100μm

武庫川女子大学 バイオサイエンス研究所 細胞生命解析学部門 蓬田健太郎先生 提供
※BIOLOGY of REPRODUCTION, Volume 67, Issue 3, Pages 712-717, September 2002 参考

文献

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エレクトロポレーション

ドラッグデリバリー・遺伝子導入

電気式細胞融合

蛍光組織染色・in situ HCR

細胞凍結

培養細胞 分離・灌流・計数・伸展・解析

微生物 計数・画像解析・検査

1細胞回収・マイクロダイセクション

In Vivo イメージング・モニタリング

卵振動培養