膵臓・ランゲルハンス島へのエレクトロポレーションによる遺伝子導入

NEPA21エレクトロポレーション装置

Sピンセット電極

ｽﾗｲﾄﾞｶﾞﾗｽ･ｼｬｰﾚ･ｶﾊﾞｰ電極

ｹｰﾌﾞﾙ･ﾌｯﾄｽｲｯﾁ

アプリケーション
文献

アプリケーション

マウスランゲルハンス島 Ex Vivo へGFPの導入結果

文献

All

膵臓

In Vivo Piggy bac-Based Gene Delivery Towards Murine Pancreatic Parenchyma Confers Sustained Expression of Gene of Interest

Masahiro Sato, Emi Inada, Issei Saitoh, Shingo Nakamura, Satoshi Watanabe

Int J Mol Sci, 20 (13) 2019 Jun 26

ランゲルハンス島
Ex_Vivo

ELKS/Voltage-Dependent Ca 2+ Channel-β Subunit Module Regulates Polarized Ca 2+ Influx in Pancreatic β Cells

Mica Ohara-Imaizumi, Kyota Aoyagi, Hajime Yamauchi, Masashi Yoshida, Masayuki X Mori, Yamato Hida, Ha Nam Tran Masamichi Ohkura, Manabu Abe, Yoshihiro Akimoto, Yoko Nakamichi, Chiyono Nishiwaki, Hayato Kawakami, Kazuo Hara, Kenji Sakimura, Shinya Nagamatsu, Yasuo Mori, Junichi Nakai, Masafumi Kakei, Toshihisa Ohtsuka

Cell Rep, 26 (5), 1213-1226.e7 2019 Jan 29

膵臓

Administration of Gemcitabine After Pancreatic Tumor Resection in Mice Induces an Antitumor Immune Response Mediated by Natural Killer Cells

Engin Gürlevik, Bettina Fleischmann-Mundt, Jennifer Brooks, Ihsan Ekin Demir, Katja Steiger, Silvia Ribback, Tetyana Yevsa, Norman Woller, Arnold Kloos, Dmitrij Ostroumov, Nina Armbrecht, Michael P Manns, Frank Dombrowski, Michael Saborowski, Moritz Kleine, Thomas C Wirth, Helmut Oettle, Güralp O Ceyhan, Irene Esposito, Diego F Calvisi, Stefan Kubicka, Florian Kühnel

Gastroenterology, 151 (2), 338-350.e7 Aug 2016

膵臓

Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice.

Maresch R, Mueller S, Veltkamp C, Öllinger R, Friedrich M, Heid I, Steiger K, Weber J, Engleitner T, Barenboim M, Klein S, Louzada S, Banerjee R, Strong A, Stauber T, Gross N, Geumann U, Lange S, Ringelhan M, Varela I, Unger K, Yang F, Schmid RM, Vassiliou GS, Braren R, Schneider G, Heikenwalder M, Bradley A, Saur D, Rad R

Nat Commun. 2016 Feb 26;7

ランゲルハンス島
Ex_Vivo

Imaging of Insulin Exocytosis from Pancreatic Beta Cells.

Ohara-Imaizumi. et al.

Exocytosis Methods, Neuromethods Volume 83, 2014

膵臓

Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice.

Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S

Biotechnol J. 2013 Aug 15. [Epub ahead of print]

ランゲルハンス島
Ex_Vivo

Acute inhibition of PI3K-PDK1-Akt pathway potentiates insulin secretion through upregulation of newcomer granule fusions in pancreatic β-cells.

Aoyagi K. et al.

PLoS One. 2012;7(10):e47381.

膵臓

Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination.

Guan X1, Nishikawa M, Takemoto S, Ohno Y, Yata T, Takakura Y

J Gene Med. 2010 Mar;12(3):301-9.

膵臓・ランゲルハンス島へのエレクトロポレーションによる遺伝子導入

アプリケーション

マウスランゲルハンス島 Ex Vivo へGFPの導入結果

文献

In Vivo Piggy bac-Based Gene Delivery Towards Murine Pancreatic Parenchyma Confers Sustained Expression of Gene of Interest

ELKS/Voltage-Dependent Ca 2+ Channel-β Subunit Module Regulates Polarized Ca 2+ Influx in Pancreatic β Cells

Administration of Gemcitabine After Pancreatic Tumor Resection in Mice Induces an Antitumor Immune Response Mediated by Natural Killer Cells

Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice.

Imaging of Insulin Exocytosis from Pancreatic Beta Cells.

Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice.

Acute inhibition of PI3K-PDK1-Akt pathway potentiates insulin secretion through upregulation of newcomer granule fusions in pancreatic β-cells.

Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination.

■ 培養細胞

■ In Vivo マウス・ラット

■ In Vivo その他の動物

■ 植物細胞・藻類

■ エクソソーム

■ バクテリア・酵母・菌類

■ ナノ粒子作製

■ 超音波（ソノポレーション・FUS）

■ ジェットインジェクション

■ パーティクルデリバリー

■ マイクロインジェクション

■ ハイブリドーマ作製

■ 卵子活性化

■ 体細胞核移植

■ 四倍体胚の作出

■ その他

■ 蛍光組織染色

■ in situ HCR

■ 幹細胞分取

■ マイクロピック＆プレースシステム

■ UnipicK・UnipicK+・A-picK

■ 細胞治療向け

■ 畜産向け

■ 微生物ライブイメージング・解析

■ リアルタイム細胞解析

■ 細胞計数分析

■ 細胞伸展培養

■ 細胞灌流培養

■ 超音波イメージング

■ 卵子・胚盤胞