網膜・角膜・脊髄・坐骨神経へのエレクトロポレーションによる遺伝子導入

NEPA21エレクトロポレーション装置

Sピンセット電極

ｹｰﾌﾞﾙ･ﾌｯﾄｽｲｯﾁ

アプリケーション
文献

アプリケーション

マウス・ラット網膜へのIn Vivoエレクトロポレーション

網膜下へDNAの注入
エレクトロポレーション

ピンセット電極
ラットをピンセット電極でホールド

様々な発生ステージで採取したラットの網膜へのエレクトロポレーション法による遺伝子導入。

図A：P0でpCAG-GFPを網膜に導入して、P21（a～c）で採取。強膜側から撮影。

図B：P0でpCAG-GFPを網膜に導入して、以下のステージで採取。
P2（d～f）、P5（g～i）

Cepko laboratory, Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School 松田孝彦先生提供

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All

In Vivo 坐骨神経

Molecular analysis of axonal-intrinsic and glial-associated co-regulation of axon degeneration

Catenaccio A, Llavero Hurtado M, Diaz P, Lamont DJ, Wishart TM, Court FA.

Cell Death Dis. 2017 Nov 9;8(11):e3166

脊髄 Ex Vivo

Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation

Mariko Kinoshita-Kawada, Hiroshi Hasegawa, Tsunaki Hongu, Shigeru Yanagi, Yasunori Kanaho, Ichiro Masai, Takayasu Mishima, Xiaoping Chen, Yoshio Tsuboi,Yi Rao, Junichi Yuasa-Kawada, Jane Y. Wu

bio-protocol Vol 9, Iss 18, September 20, 2019

網膜 Ex Vivo

Quantitative Analysis of the ThrbCRM1-centered Gene Regulatory Network

Benjamin Souferi, Mark M Emerson

Biol Open, 8 (4) 2019 Apr 26

脊髄 In Vivo

Convallatoxin Enhance the Ligand-Induced Mu-Opioid Receptor Endocytosis and Attenuate Morphine Antinociceptive Tolerance in Mice

Po-Kuan Chao, Hsiao-Fu Chang, Li-Chin Ou, Jian-Ying Chuang, Pin-Tse Lee, Wan-Ting Chang, Shu-Chun Chen, Shau-Hua Ueng, John Tsu-An Hsu, Pao-Luh Tao, Ping-Yee Law, Horace H Loh, Shiu-Hwa Yeh

Sci Rep, 9 (1), 2405 2019 Feb 20

脊髄 Ex Vivo

A crucial role for Arf6 in the response of commissural axons to Slit

Kinoshita-Kawada M, Hasegawa H, Hongu T, Yanagi S, Kanaho Y, Masai I, Mishima T, Chen X, Tsuboi Y, Rao Y, Yuasa-Kawada J, Wu JY

Development. 2019 Feb 4;146(3).

網膜 In Vivo/Ex Vivo

Gene Transfer of Prolyl Hydroxylase Domain 2 Inhibits Hypoxia-inducible Angiogenesis in a Model of Choroidal Neovascularization.

Takei A, Ekström M, Mammadzada P, Aronsson M, Yu M, Kvanta A, André H

Sci Rep. 2017 Feb 10;7:42546.

脊髄 In Vivo

Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling.

Lee PT, Chao PK, Ou LC, Chuang JY, Lin YC, Chen SC, Chang HF, Law PY, Loh HH, Chao YS, Su TP, Yeh SH

Nucleic Acids Res. 2014 Dec 1;42(21):13012-25.

坐骨神経

Schwann cell-derived exosomes enhance axonal regeneration in the peripheral nervous system.

Lopez-Verrilli MA et al

Glia. 2013 Nov;61(11):1795-806.

網膜 In Vivo/Ex Vivo

Requirement of retinoic acid receptor β for genipin derivative-induced optic nerve regeneration in adult rat retina.

Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Sugitani K, Arai K, Suzuki H, Kato S.

PLoS One. 2013 Aug 6;8(8):e71252.

脊髄 In Vivo

CCL-1 in the spinal cord contributes to neuropathic pain induced by nerve injury.

Akimoto N et al.

Cell Death Dis. 2013 Jun 20

網膜 Ex Vivo

Identification of a Retina-Specific Otx2 Enhancer Element Active in Immature Developing Photoreceptors

Mark M Emerson, Constance L Cepko

Dev Biol, 360 (1), 241-55 2011 Dec 1

網膜 Ex Vivo

Bulk electroporation and population calcium imaging in the adult mammalian retina.

Briggman KL, Euler T.

J Neurophysiol. 2011 May;105(5):2601-9.

網膜 Ex Vivo

Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.

Ozawa Y et al.

J Biol Chem. 2008 Sep 5;283(36):24561-70.

網膜 In Vivo/Ex Vivo

A role for ligand-gated ion channels in rod photoreceptor development

Tracy L. Young and Constance L. Cepko

Neuron . 2004 Mar 25;41(6):867-79.

網膜 In Vivo/Ex Vivo

Electroporation and RNA interference in the rodent retina in vivo and in vitro

Takahiko Matsuda and Constance L. Cepko

Proc Natl Acad Sci U S A . 2004 Jan 6;101(1):16-22.

角膜

Targeted gene transfer to corneal stroma in vivo by electric pulses

Oshima, et al.

Experimental Eye Research, Volume 74, Issue 2, Pages 191-198, February 2002

網膜 In Vivo/Ex Vivo

Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach

Dezawa et al.

Micron . 2002;33(1):1-6.

網膜・角膜・脊髄・坐骨神経へのエレクトロポレーションによる遺伝子導入

アプリケーション

マウス・ラット網膜へのIn Vivoエレクトロポレーション

文献

Molecular analysis of axonal-intrinsic and glial-associated co-regulation of axon degeneration

Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation

Quantitative Analysis of the ThrbCRM1-centered Gene Regulatory Network

Convallatoxin Enhance the Ligand-Induced Mu-Opioid Receptor Endocytosis and Attenuate Morphine Antinociceptive Tolerance in Mice

A crucial role for Arf6 in the response of commissural axons to Slit

Gene Transfer of Prolyl Hydroxylase Domain 2 Inhibits Hypoxia-inducible Angiogenesis in a Model of Choroidal Neovascularization.

Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling.

Schwann cell-derived exosomes enhance axonal regeneration in the peripheral nervous system.

Requirement of retinoic acid receptor β for genipin derivative-induced optic nerve regeneration in adult rat retina.

CCL-1 in the spinal cord contributes to neuropathic pain induced by nerve injury.

Identification of a Retina-Specific Otx2 Enhancer Element Active in Immature Developing Photoreceptors

Bulk electroporation and population calcium imaging in the adult mammalian retina.

Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.

A role for ligand-gated ion channels in rod photoreceptor development

Electroporation and RNA interference in the rodent retina in vivo and in vitro

Targeted gene transfer to corneal stroma in vivo by electric pulses

Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach

■ 培養細胞

■ In Vivo マウス・ラット

■ In Vivo その他の動物

■ 植物細胞・藻類

■ エクソソーム

■ バクテリア・酵母・菌類

■ ナノ粒子作製

■ 超音波（ソノポレーション・FUS）

■ ジェットインジェクション

■ パーティクルデリバリー

■ マイクロインジェクション

■ ハイブリドーマ作製

■ 卵子活性化

■ 体細胞核移植

■ 四倍体胚の作出

■ その他

■ 蛍光組織染色

■ in situ HCR

■ 幹細胞分取

■ マイクロピック＆プレースシステム

■ UnipicK・UnipicK+・A-picK

■ 細胞治療向け

■ 畜産向け

■ 微生物ライブイメージング・解析

■ リアルタイム細胞解析

■ 細胞計数分析

■ 細胞伸展培養

■ 細胞灌流培養

■ 超音波イメージング

■ 卵子・胚盤胞