Insects・Others

NEPA21

Petridish/Microscope Slide Electrodes

Needle/Other Electrodes

Cables, Foot Switch & Others

Insects・Others

APPLICATIONS

In vivo gene transfer into the adult honeybee brain by using electroporation

     honey bee electroporation      honey bee electroporation

 

♦In vivo electroporation.

(A) The necks of honeybees that had settled in the tube were fixed in place by inserting two U-shaped plastic sheets for surgical ease and later release.
(B) A honeybee settled in the tube with her neck fixed prior to the operation.
(C) Schematic representation of the micropipette and electrodes placed in the honeybee brain. Purple indicates micropipette filled with DNA solution, blue indicates electrodes. AN, antennae; C, compound eyes; MB, mushroom bodies; Oc, ocelli; OL, optic lobes.
(D) DNA injection and electroporation with needle electrodes.

Arrows and arrowhead indicate electrodes and micropipette for DNA injection, respectively. Scale bar, 300 µm.

 

honey bee brain

 

Fluorescent images of electroporated honeybee brain at 50 V, targeting the optic lobe (A, B) and mushroom bodies (C-F).
Fluorescent signals were detected around the anode position in the brain electroporated with GFP-expressing plasmid driven by the CMV promoter (A, C, E, F) and not in those electroporated without plasmid (B, D).
(G, H) Magnified views of GFP-expressing areas shown in (A) and (F), respectively.
Positions of electrodes are indicated by circles containing + or -.
Arrowheads indicate the regions in which fluorescence was detected (A, C, E, G) and neural projections from mushroom body cells (F, H).
Frontal view (A, B, F, G, H), rear view (C, D), and top view (E).
Panels (C) and (E) show different views of the same brain.

 

Takekazu Kunieda and Takeo Kubo, Department of Biological Sciences, Graduate School of Science, University of Tokyo
*University of Tokyo Patent Pending
*Biochemical and Biophysical Research Communications, Volume 318, Issue 1, Pages 25-31, May 21, 2004

PUBLICATIONS

  • P.xuthus_caterpillar

Prepatterning of Papilio xuthus caterpillar camouflage is controlled by three homeobox genes: clawless, abdominal-A, and Abdominal-B

Jin H, Seki T, Yamaguchi J, Fujiwara H.

Sci Adv. 2019 Apr 10;5(4):eaav7569.

  • Cricket

Targeted gene delivery in the cricket brain, using in vivo electroporation.

Matsumoto CS, Shidara H, Matsuda K, Nakamura T, Mito T, Matsumoto Y, Oka K, Ogawa H.

J Insect Physiol. 2013 Dec;59(12):1235-41.

  • Honeybee

Honey bee promoter sequences for targeted gene expression.

Schulte C, Leboulle G, Otte M, Grünewald B, Gehne N, Beye M.

Insect Mol Biol. 2013 Aug;22(4):399-410.

  • Xenopus_laevis
  • Zygotes

A versatile protocol for mRNA electroporation of Xenopus laevis embryos

Chernet BT, Levin M.

Cold Spring Harb Protoc. 2012 Apr 1;2012(4):447-52.

  • Watar_Flea

Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation.

Kato Y, Kobayashi K, Watanabe H, Iguchi T.

Ecotoxicolog. 2010 Mar;19(3):589-92.

  • Honeybee

In vivo gene transfer into the adult honeybee brain by using electroporation

Kunieda T, Kubo T.

Biochem Biophys Res Commun. 2004 May 21;318(1):25-31.

  • Planarian

Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker

González-Estévez C, Momose T, Gehring WJ, Saló E.

Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14046-51.

  • Xenopus
  • Zygotes

Improved mRNA electroporation method for Xenopus neurula embryos

Sasagawa S, Takabatake T, Takabatake Y, Muramatsu T, Takeshima K.

Genesis. 2002 Jun;33(2):81-5.

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration