Freezing of stem cells, primary cells, cord blood, etc.
Accurate temperature control allows for high cell viability.
Viability after freezing
Viability rates after freezing human dental pulp stem cells (DPSCs), human periodontal ligament stem cells (PDLFs), human bone marrow stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs)
Each cell was suspended according to the protocol of the cryoprotectant, filled into 2-mL cryo tubes, and cooled to -80°C at -1°C/min in PF-NP-200, resulting in high viability rates.
Mouse proB lymphocyte cells (Ba/F3)
Ba/F3 cells were suspended according to the protocol of the freezing agent, filled into freeze bags, and cooled to -80°C in a PF-NP freezer at -1°C/min, resulting in a high survival rate comparable to that of 2-mL cryovials.
*Collaboration with Excellent Young Researcher (LEADER) Shinya Yamahira, St. Luke’s International Univrsity, Center for Medical Sciences