Freezing of Stem Cells, Primary Cells, Cord Blood, etc.

PF-NP-200

Accurate temperature control ensures high cell viability.

Freezing of Stem Cells, Primary Cells, Cord Blood, etc.

APPLICATIONS

High Cell Viability After Freezing

hDPSCs, hPDLFs, hBMSCs, HUVEC

Each cell type was suspended according to the cryoprotectant protocol, transferred into 2-mL cryovials, and cooled to -80°C at a rate of -1°C/min using the PF-NP-200. The results demonstrated high cell viability rates.

Freezer viability cryovial

human dental pulp stem cells (DPSCs), human periodontal ligament stem cells (PDLFs), human bone marrow stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs)

Mouse proB lymphocyte cells (Ba/F3)

Ba/F3 cells were suspended according to the freezing agent protocol, transferred into cell culture bags, and cooled to -80°C at -1°C/min using a PF-NP freezer. The results showed a high cell survival rate comparable to that of 2-mL cryovials.

Freezer viability cell culture bag

 

*Collaboration with Excellent Young Researcher (LEADER) Shinya Yamahira, St. Luke’s International University, Center for Medical Sciences

PUBLICATIONS

  • Human iPS cells

Detection of residual pluripotent stem cells in cell therapy products utilizing droplet digital PCR: an international multisite evaluation study

Satoshi Yasuda, Kiyoko Bando, Marianne P Henry, Silvana Libertini, Takeshi Watanabe, Hiroto Bando, Connie Chen, Koki Fujimori, Kosuke Harada, Takuya Kuroda, Myriam Lemmens, Dragos Marginean, David Moss, Lucilia Pereira Mouriès, Nicole S Nicholas, Matthew J K Smart, Orie Terai, Yoji Sato

Stem Cells Transl Med . 2024 Aug 9:szae058.

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration