ECFG21 Super Electro Cell Fusion Generator
Hybridoma Production, Somatic-Cell Nuclear Transfer and More
- High-performance, Downsized, and Lightweight by the latest technology
- The world first device that can automatically read out delivered voltage and current both AC & DC pulses.
- The fusion efficiency is 3 to 6 times higher than PEG!!
- Besides, the new electrode chambers correspond to a large volume solution.
APPLICATIONS
Electro Cell Fusion
Hybridoma Production
- Monoclonal antibodies, etc
- lymphocytes and myeloma cells for monoclonal antibodies
- *a large volume chamber is available.
Oocyte Activation
- Electrical stimulation before/after Intracytoplasmic sperm injection (ICSI)
Oocyte activation by electrical stimulation
Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer
- Animal cloning
Pig, Cattle, Dog, Cat, Boar, and other animals
Tetraploid Embryos Production
Other Applications
- Liposome・Protoplast・Yeast, etc.
Liposome, Protoplast, Yeast, etc.
PRODUCT DESCRIPTION
AC → 2-Step DC Pulses with Voltage Decay → Post Fusion AC
- AC (Alternating Current): AC is applied so that the lymphocyte cells (producing antibodies) ＆ myeloma cells are aligned in a chain for touching on each side: ”Pearl Chain” Formation
- 2-Step DC (Direct Current) Pulses with Voltage Decay: Pores form in the aligned cells membranes are high-efficiency by 2-Step DC Pulses with Voltage Decay (Multiple pulses・Decay rate・Polarity exchanged).
- Post fusion (AC): The post fusion AC is applied so that the fusion process begins to mature and high fusion efficiency is achieved with low cell damage.
Electro Cell Fusion Process
The world first device that can automatically read out delivered voltage and current both AC & DC pulses.
Check the parameters not only setting, but also automatically read out delivered voltage and current both AC & DC pulses & measurements voltage/current,
it is able to achieve the high efficient cell fusion.
ECFG21 Specifications
Alternate Current (AC)
| Voltage | 0 – 80 Vrms |
| Frequency | 1 MHz |
| Duration | 0 – 99 sec |
| Post-Fusion Duration | 0 – 99 sec |
| Post-Fusion Decay Mode | On/Off |
| Pause between AC / DC | 5 μsec |
Direct Current (DC) Pulse
| Pulse Wave | Square Wave |
| Voltage | 1 – 1500 V |
| Pulse Length | 1 – 99 μsec |
| Pulse Interval | 0.1 – 9.9 sec |
| Number of Pulses | 0 – 99 |
| Decay Rate | 0 – 99 % |
| Polarity Switching | On/Off |
Measurements
| Voltage | AC / DC |
| Current | AC / DC |
| Energy (J) | DC |
Others
| Impedance Measurement | 0.01 – 50 kΩ |
| Operation Mode | Automatic / Manual |
| Memory | 99 programs |
| Dimensions / Weight | 386W x 370D x 121H mm / 8.6kg |
All features and specifications subject to change without notice.
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