Transfection into Primary Cell Cultures by Electroporation

Transfection into Primary Cell Cultures by Electroporation

APPLICATIONS

Transfection Results with Nepa Gene Electroporation System

BMMC  Primary Mouse Bone Marrow-Derived Mast cells

Viability: 80%

Transfection Efficiency: 83%

FACS

bmmc_c-1

Primary Mouse Cerebral Cortex Neurons (E14)

Viability: 80%

Transfection Efficiency: 70%

Primary Human Endometrial Stromal Cells

Viability: 95%

Transfection Efficiency: 90%

Transfection Data: Primary Huyman Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
HUVEC Human Umbilical Vein Endothelial Cells95%75% HUVEC Human Umbilical Vein Endothelial Cells100%92%
HASM Human Airway Smooth Muscle Cells90%80% Human Coronary-derived Smooth Muscle Cells82%67%
Human Endometrial Stromal Cells95%90% Human Uterine Cervical Fibroblasts65%90%
Human Dermal Fibroblasts (HDF)95%89% Human Dermal Fibroblasts (HDF)100%80%
Human Keratinocytes70%65% Human Malignant Mesothelioma Cells75%55%
Human Meniscal Cells85%55% Human Colorectal Cells53%80%
Human T Cells58%90% Human T Cells50%76%
Human NK Cells48%86% PBMC Peripheral Blood Mononuclear Cells93%66%
Human Erythroblasts (CD34+ Progenitor Cells)61%34% Human Chronic Lymphocytic Leukemia (CLL)82%70%
Human Chronic Lymphocytic Leukemia (CLL) (mRNA)93%94% Human Chronic Lymphocytic Leukemia (CLL)91%83%
Human Chronic Lymphocytic Leukemia (CLL)83%93% Human Osteoblast-like Cells derived from human skull64%74%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Primary Mouse Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Mouse Cerebral Cortex Neurons (E14)80%70% Mouse Hippocampal Neurons (E14)80%60%
Mouse Hippocampal Neurons (E17)65%70% Mouse Neural Progenitor Cells80%60%
Mouse Basal Ganglia Primordium91%71% Mouse Cerebellar Granule Neurons91%65%
Mouse DRG Neurons83%68% Mouse DRG Neurons70%70%
MEF Mouse Embryonic Fibroblasts90%85% MEF Mouse Embryonic Fibroblasts75%85%
Mouse Embryonic Skin Fibroblasts80%50%    
Mouse External Genital Fibroblasts (E15.5)66%59% Mouse Cervical Epithelial Cells82%55%
Mouse Vascular Adventitial Fibroblasts90%50% BMMC Mouse Bone Marrow-Derived Mast Cells80%83%
Mouse peritoneal macrophages69%41% Mouse B cells (LPS stimulated)81%73%
Mouse B cells50%61% Mouse B cells (Unstimulated)84%83%
Mouse T cells (siRNA)88%77% Naive Mouse CD8+ T cells (Cas9 RNP)50%95%
Mouse Liver Cells75%65% Mouse Osteoblast Cells85%60%
Mouse Muscle Cells68%54%    

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Primary Rat Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Rat Cerebral Cortex Neurons (E16)70%75% Rat Hippocampal Neurons60%80%
Rat Bulbar Neurons80%75% Rat Cerebellar Neurons70%55%
Rat Cerebellar Granule Cells70%80% Rat Schwann Cells90%80%
Rat Schwann Cells90%60% OEC Rat Olfactory Ensheathing Cells93%46%
Rat Müller Cells90%50% REF Rat Embryonic Fibroblasts65%65%
Rat Meningeal Fibroblasts90%95% PASMC Rat Pulmonary Artery Smooth Muscle Cells72%70%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Other Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Chick Embryonic Fibroblasts80%90% Chick Embryonic Cerebellar Granule Cells86%83%
Bovine Cartilage Cells96%55% Goat Embryonic Epithelial Fibroblasts80%55%
Rabbit Spleen cells (B cells)70%45% Canine Adipose-Derived Stromal Cells (ADSCs) 65%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescence Quenching / in situ Hybridization Chain Reaction

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration