Transfection into Primary Cell Cultures by Electroporation
APPLICATIONS
Transfection Results with Nepa Gene Electroporation System
BMMC Primary Mouse Bone Marrow-Derived Mast cells

Viability: 80%

Transfection Efficiency: 83%
FACS
Primary Mouse Cerebral Cortex Neurons (E14)

Viability: 80%

Transfection Efficiency: 70%
Primary Human Endometrial Stromal Cells

Viability: 95%

Transfection Efficiency: 90%
Transfection Data: Primary Huyman Cells
See the cell images by clicking the cell names.
V: Viability, TE: Transfection Efficiency.
Primary Cells | V | TE | Primary Cells | V | TE | |
HUVEC Human Umbilical Vein Endothelial Cells | 95% | 75% | HUVEC Human Umbilical Vein Endothelial Cells | 100% | 92% | |
HASM Human Airway Smooth Muscle Cells | 90% | 80% | Human Coronary-derived Smooth Muscle Cells | 82% | 67% | |
Human Endometrial Stromal Cells | 95% | 90% | Human Uterine Cervical Fibroblasts | 65% | 90% | |
Human Dermal Fibroblasts (HDF) | 95% | 89% | Human Dermal Fibroblasts (HDF) | 100% | 80% | |
Human Keratinocytes | 70% | 65% | Human Malignant Mesothelioma Cells | 75% | 55% | |
Human Meniscal Cells | 85% | 55% | Human Colorectal Cells | 53% | 80% | |
Human T Cells | 58% | 90% | Human T Cells | 50% | 76% | |
Human NK Cells | 48% | 86% | PBMC Peripheral Blood Mononuclear Cells | 93% | 66% | |
Human Erythroblasts (CD34+ Progenitor Cells) | 61% | 34% | Human Chronic Lymphocytic Leukemia (CLL) | 82% | 70% | |
Human Chronic Lymphocytic Leukemia (CLL) (mRNA) | 93% | 94% | Human Chronic Lymphocytic Leukemia (CLL) | 91% | 83% | |
Human Chronic Lymphocytic Leukemia (CLL) | 83% | 93% | Human Osteoblast-like Cells derived from human skull | 64% | 74% |
We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.
Transfection Data: Primary Mouse Cells
See the cell images by clicking the cell names.
V: Viability, TE: Transfection Efficiency.
Primary Cells | V | TE | Primary Cells | V | TE | |
Mouse Cerebral Cortex Neurons (E14) | 80% | 70% | Mouse Hippocampal Neurons (E14) | 80% | 60% | |
Mouse Hippocampal Neurons (E17) | 65% | 70% | Mouse Neural Progenitor Cells | 80% | 60% | |
Mouse Basal Ganglia Primordium | 91% | 71% | Mouse Cerebellar Granule Neurons | 91% | 65% | |
Mouse DRG Neurons | 83% | 68% | Mouse DRG Neurons | 70% | 70% | |
MEF Mouse Embryonic Fibroblasts | 90% | 85% | MEF Mouse Embryonic Fibroblasts | 75% | 85% | |
Mouse Embryonic Skin Fibroblasts | 80% | 50% | ||||
Mouse External Genital Fibroblasts (E15.5) | 66% | 59% | Mouse Cervical Epithelial Cells | 82% | 55% | |
Mouse Vascular Adventitial Fibroblasts | 90% | 50% | BMMC Mouse Bone Marrow-Derived Mast Cells | 80% | 83% | |
Mouse peritoneal macrophages | 69% | 41% | Mouse B cells (LPS stimulated) | 81% | 73% | |
Mouse B cells | 50% | 61% | Mouse B cells (Unstimulated) | 84% | 83% | |
Mouse T cells (siRNA) | 88% | 77% | Naive Mouse CD8+ T cells (Cas9 RNP) | 50% | 95% | |
Mouse Liver Cells | 75% | 65% | Mouse Osteoblast Cells | 85% | 60% | |
Mouse Muscle Cells | 68% | 54% |
We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.
Transfection Data: Primary Rat Cells
See the cell images by clicking the cell names.
V: Viability, TE: Transfection Efficiency.
Primary Cells | V | TE | Primary Cells | V | TE | |
Rat Cerebral Cortex Neurons (E16) | 70% | 75% | Rat Hippocampal Neurons | 60% | 80% | |
Rat Bulbar Neurons | 80% | 75% | Rat Cerebellar Neurons | 70% | 55% | |
Rat Cerebellar Granule Cells | 70% | 80% | Rat Schwann Cells | 90% | 80% | |
Rat Schwann Cells | 90% | 60% | OEC Rat Olfactory Ensheathing Cells | 93% | 46% | |
Rat Müller Cells | 90% | 50% | REF Rat Embryonic Fibroblasts | 65% | 65% | |
Rat Meningeal Fibroblasts | 90% | 95% | PASMC Rat Pulmonary Artery Smooth Muscle Cells | 72% | 70% |
We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.
Transfection Data: Other Primary Cells
See the cell images by clicking the cell names.
V: Viability, TE: Transfection Efficiency.
Primary Cells | V | TE | Primary Cells | V | TE | |
Chick Embryonic Fibroblasts | 80% | 90% | Chick Embryonic Cerebellar Granule Cells | 86% | 83% | |
Bovine Cartilage Cells | 96% | 55% | Goat Embryonic Epithelial Fibroblasts | 80% | 55% | |
Rabbit Spleen cells (B cells) | 70% | 45% | Canine Adipose-Derived Stromal Cells (ADSCs) | 65% |
We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.
PUBLICATIONS
- Primary_rat_neuronal_cells
The Transcription Factor Ets1 Influences Axonal Growth via Regulation of Lcn2
Gu M, Li X, Wu R, Cheng X, Zhou S, Gu X.
Mol Neurobiol. 2024 Feb;61(2):971-981.
- Human_nasal_epithelial_cells(HNEC)
Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia
Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia
Life Sci Alliance. 2022 Aug 3;5(12):e202101320.
Electroporation
■ Cell Cultures
- Primary Cell Cultures
- Stem Cells
- Organoids
- Cell Lines
- Cells in Adherence
■ In Vivo Mice/Rats
- Zygotes In Vitro (TAKE method)
- Zygotes In Oviduct (i-GONAD method)
- Embryos In Utero
- Ex Utero Embryos In Vitro
- Brain
- Retina / Cornea / Spinal Cord / Sciatic Nerve
- Lung / Spleen / Liver / Stomach/ Kidney / Intestine
- Pancreas / Islets of langerhans
- Testis / Ovary / Prostate / Gonad / Uterus
- Muscle / Skin / Joint / Cartilage / Tumor / Others
■ In Vivo Other Animals
- Bovine/Porcine/Other Animal Zygotes
- Hamster Zygotes in Oviduct (i-GONAD method)
- Monkey Skin
- Chicken (In Ovo・Others)
- Zebrafish & Other Fishes
- Insects・Others
■ Plant Cells & Algae
- Plant Cells
- Algae
■ Exosomes
- Exosomes
■ Bacteria, Yeast, Fungi
- E. coli/Bacterial Cells
- Yeasts/Fungi
- Bacterial cells/Yeasts/Fungi (NEPA Porator)
Drug Delivery and Transfection
■ Ultrasound Transfection and Drug Delivery (Sonoporation/Fus)
- Brain
- Liver/Skin/Other Applications
- Heart
- Cell Culture
- Lung
- Muscle
Electro Cell Fusion
■ Hybridoma Production
- Monoclonal antibodies, etc
■ Oocyte Activation
- Electrical stimulation before/after Intracytoplasmic sperm injection (ICSI)
■ Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer
- Animal cloning
■ Tetraploid Embryos Production
- 2 Cell Embryos (Tetraploid)
■ Other Applications
- Liposome・Protoplast・Yeast, etc.
Fluorescence Quenching / in situ Hybridization Chain Reaction
■ Autofluorescence Quenching
- Mammalian Tissue Sections
- Fish Tissue Sections
- Amphibia Tissue Sections
- Avian Tissue Sections
- Plant Tissue Sections
- Chordate Tissue Sections
■ in situ HCR
- Detection of Target mRNA
Single-Cell/Micro-Particle Transfer
■ マイクロピック&プレースシステム
- Picking and placing micro targets
■ Micro targets
- Animal cells
■ Micro liquid
- Plant cells
Cell Freezing
■ Cell Therapy
- Stem cells, primary cells, and more
■ Animal Husbandry
- Sperm, embryos, tissues, and more