Transfection into Primary Cell Cultures by Electroporation

NEPA21

ELEPO21

Cuvette Chambers ＆ Stand Holder

Nepa Electroporation Cuvettes

Nepa Monitoring System

Transfection into Primary Cell Cultures by Electroporation

APPLICATIONS
PUBLICATIONS

APPLICATIONS

Transfection Results with Nepa Gene Electroporation System

BMMC Primary Mouse Bone Marrow-Derived Mast cells

Viability: 80%

Transfection Efficiency: 83%

FACS

Primary Mouse Cerebral Cortex Neurons (E14)

Viability: 80％

Transfection Efficiency: 70％

Primary Human Endometrial Stromal Cells

Viability: 95％

Transfection Efficiency: 90％

Transfection Data: Primary Huyman Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells	V	TE	Primary Cells	V	TE
HUVEC Human Umbilical Vein Endothelial Cells	95%	75%	HUVEC Human Umbilical Vein Endothelial Cells	100%	92%
HASM Human Airway Smooth Muscle Cells	90%	80%	Human Coronary-derived Smooth Muscle Cells	82%	67%
Human Endometrial Stromal Cells	95%	90%	Human Uterine Cervical Fibroblasts	65%	90%
Human Dermal Fibroblasts (HDF)	95%	89％	Human Dermal Fibroblasts (HDF)	100%	80%
Human Keratinocytes	70%	65%	Human Malignant Mesothelioma Cells	75％	55％
Human Meniscal Cells	85%	55%	Human Colorectal Cells	53%	80%
Human T Cells	58%	90%	Human T Cells	50%	76%
Human NK Cells	48%	86%	PBMC Peripheral Blood Mononuclear Cells	93%	66%
Human Erythroblasts (CD34+ Progenitor Cells)	61％	34％	Human Chronic Lymphocytic Leukemia (CLL)	82%	70%
Human Chronic Lymphocytic Leukemia (CLL) (mRNA)	93％	94%	Human Chronic Lymphocytic Leukemia (CLL)	91%	83%
Human Osteoblast-like Cells derived from human skull	64%	74%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

Transfection Data: Primary Mouse Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells	V	TE	Primary Cells	V	TE
Mouse Cerebral Cortex Neurons (E14)	80%	70%	Mouse Hippocampal Neurons (E14)	80%	60%
Mouse Hippocampal Neurons (E17)	65%	70%	Mouse Neural Progenitor Cells	80%	60%
Mouse Basal Ganglia Primordium	91%	71%	Mouse Cerebellar Granule Neurons	91%	65%
Mouse DRG Neurons	70%	70%	MEF Mouse Embryonic Fibroblasts	90%	85%
MEF Mouse Embryonic Fibroblasts	75%	85%	Mouse Embryonic Skin Fibroblasts	80%	50%
Mouse External Genital Fibroblasts (E15.5)	66%	59%	Mouse Cervical Epithelial Cells	82%	55%
Mouse Vascular Adventitial Fibroblasts	90%	50%	BMMC Mouse Bone Marrow-Derived Mast Cells	80%	83%
Mouse peritoneal macrophages	69%	41%	Mouse B cells (LPS stimulated)	81%	73%
Mouse B cells	50%	61%	Mouse B cells (Unstimulated)	84%	83%
Mouse T cells (siRNA)	88%	77%	Naive Mouse CD8+ T cells (Cas9 RNP)	50%	95%
Mouse Liver Cells	75%	65%	Mouse Osteoblast Cells	85%	60%
Mouse Muscle Cells	68%	54%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

Transfection Data: Primary Rat Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells	V	TE	Primary Cells	V	TE
Rat Cerebral Cortex Neurons (E16)	70%	75%	Rat Hippocampal Neurons	60%	80%
Rat Bulbar Neurons	80%	75%	Rat Cerebellar Neurons	70%	55%
Rat Cerebellar Granule Cells	70%	80%	Rat Schwann Cells	90%	80%
Rat Schwann Cells	90%	60%	OEC Rat Olfactory Ensheathing Cells	93%	46%
Rat Müller Cells	90%	50%	REF Rat Embryonic Fibroblasts	65%	65%
Rat Meningeal Fibroblasts	90%	95%	PASMC Rat Pulmonary Artery Smooth Muscle Cells	72%	70%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

Transfection Data: Other Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells	V	TE	Primary Cells	V	TE
Chick Embryonic Fibroblasts	80%	90%	Chick Embryonic Cerebellar Granule Cells	86%	83%
Bovine Cartilage Cells	96%	55%	Goat Embryonic Epithelial Fibroblasts	80%	55%
Rabbit Spleen cells (B cells)	70%	45%	Canine Adipose-Derived Stromal Cells (ADSCs)		65%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

PUBLICATIONS

All

Primary_rat_neuronal_cells

The Transcription Factor Ets1 Influences Axonal Growth via Regulation of Lcn2

Gu M, Li X, Wu R, Cheng X, Zhou S, Gu X.

Mol Neurobiol. 2024 Feb;61(2):971-981.

Primary_mouse_hematopoietic_stem_cells_(HSCs)

Non-viral ex vivo genome-editing in mouse bona fide hematopoietic stem cells with CRISPR/Cas9

HoSuvd Byambaa, Hideki Uosaki, Tsukasa Ohmori, Hiromasa Hara, Hitoshi Endo, Osamu Nureki, Yutaka Hanazono

Mol Ther Methods Clin Dev. 2021 Jan 9:20:451-462.

Primary Human NK Cells

CXCR1 Expression to Improve Anti-Cancer Efficacy of Intravenously Injected CAR-NK Cells in Mice With Peritoneal Xenografts

Yu Yang Ng, Johan C K Tay, Shu Wang

Mol Ther Oncolytics, 16, 75-85 2019 Dec 24

Primary Human Hepatocytes

CXCR4 Regulates Plasmodium Development in Mouse and Human Hepatocytes

Hironori Bando, Ariel Pradipta, Shiroh Iwanaga, Toru Okamoto, Daisuke Okuzaki, Shun Tanaka, Joel Vega-Rodríguez, Youngae Lee, Ji Su Ma, Naoya Sakaguchi, Akira Soga, Shinya Fukumoto, Miwa Sasai, Yoshiharu Matsuura, Masao Yuda, Marcelo Jacobs-Lorena, Masahiro Yamamoto

J Exp Med. 2019 Aug 5;216(8):1733-1748.

Primary Human NK Cells

Adoptive Transfer of NKG2D CAR mRNA-Engineered Natural Killer Cells in Colorectal Cancer Patients

Lin Xiao, Dongzhi Cen, Haining Gan, Yan Sun, Nanqi Huang, Hanzhen Xiong, Qiongmei Jin, Liqun Su, Xuejuan Liu, Kejian Wang, Guangrong Yan, Tianfa Dong, Shangbiao Wu, Pengzhi Zhou, Jinshan Zhang, Weixiang Liang, Junlan Ren, Yaoshu Teng, Can Chen, Xue Hu Xu

Mol Ther, 27 (6), 1114-1125 2019 Jun 5

Primary Human T cells

Large-scale expansion of Vγ9Vδ2 T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor-modified effector cells.

Xiao L, Chen C, Li Z, Zhu S, Tay JC, Zhang X, Zha S, Zeng J, Tan WK, Liu X, Chng WJ, Wang S

Cytotherapy. 2018 Mar;20(3):420-435.

Primary Mouse Neurons Cells

OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex

Huang B, Li X, Tu X, Zhao W, Zhu D, Feng Y, Si X, Chen JG

J Biol Chem. 2018 Feb 9;293(6):2137-2148.

Primary Human Skin Fibroblasts

Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia

Lesport E, Ferster A, Biver A, Roch B, Vasquez N, Jabado N, Vives FL, Revy P, Soulier J, de Villartay JP

Oncotarget. 2017 Dec 17;9(3):3779-3793.

PBMC and Primary Human T Cells

Enhancement of antitumor activity by using a fully human gene encoding a single-chain fragmented antibody specific for carcinoembryonic antigen.

Shibaguchi H, Luo N, Shirasu N, Kuroki M, Kuroki M

Onco Targets Ther. 2017 Aug 22;10:3979-3990.

Primary Mouse Neurons Cells

Endocytosis following dopamine D2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFRβ signaling in striatopallidal medium spiny neurons

Shioda N, Yabuki Y, Wang Y, Uchigashima M, Hikida T, Sasaoka T, Mori H, Watanabe M, Sasahara M, Fukunaga K

Mol Psychiatry. 2017 Aug;22(8):1205-1222.

Primary Human Skin Fibroblasts

A sister of NANOG regulates genes expressed in pre-implantation human development.

Dunwell TL, Holland PWH

Open Biol. 2017 Apr;7(4). pii: 170027.

Primary Rhesus Macaque CD4+ T cells

Targeting the rhesus macaque TRIM5α gene to enhance the susceptibility of CD4+ T cells to HIV-1 infection

Wang X, Yu Q, Yuan Y, Teng Z3, Li D, Zeng Y

Arch Virol. 2017 Mar;162(3):793-798.

Primary Human T cells

Intraperitoneal immunotherapy with T cells stably and transiently expressing anti-EpCAM CAR in xenograft models of peritoneal carcinomatosis

Ang WX, Li Z, Chi Z, Du SH, Chen C, Tay JC1, Toh HC, Connolly JE, Xu XH, Wang S

Oncotarget. 2017 Feb 21;8(8):13545-13559.

Primary Mouse Astrocytes

A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury

Ishii T, Ueyama T, Shigyo M, Kohta M, Kondoh T, Kuboyama T, Uebi T, Hamada T, Gutmann DH, Aiba A, Kohmura E, Tohda C, Saito N

J Biol Chem. 2017 Jan 27;292(4):1240-1250.

Primary Mouse Bone Marrow-Derived Mast cells (BMMCs)

Real-time imaging of mast cell degranulation in vitro and in vivo

Horiguchi K, Yoshikawa S, Saito A, Haddad S, Ohta T, Miyake K, Yamanishi Y, Karasuyama H

Biochem Biophys Res Commun. 2016 Oct 21;479(3):517-522.

Primary Human T cells

Co-Expansion of Cytokine-Induced Killer Cells and Vγ9Vδ2 T Cells for CAR T-Cell Therapy

Du SH, Li Z, Chen C, Tan WK, Chi Z, Kwang TW, Xu XH, Wang S

PLoS One. 2016 Sep 6;11(9):e0161820.

Primary Rat Neurons Cells

Expression of Channelrhodopsin-2 Using in Suspension Electroporation for Studying the Monosynaptic Transmission in Neuronal Culture

Natalia Bal, Aleksey Malyshev, Ivan Smirnov, Pavel Balaban

BioNanoScience 6, 329–331(2016)

Primary Human Skin Fibroblasts

Evolutionary origin and functional divergence of totipotent cell homeobox genes in eutherian mammals

Maeso I, Dunwell TL, Wyatt CD, Marlétaz F, Vető B, Bernal JA, Quah S, Irimia M, Holland PW

BMC Biol. 2016 Jun 13;14:45.

Primary Porcine Dermal Fibroblasts, Kidney Fibroblasts, and Myoblasts

Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets

Rao S, Fujimura T, Matsunari H, Sakuma T, Nakano K, Watanabe M, Asano Y, Kitagawa E, Yamamoto T, Nagashima H

Mol Reprod Dev. 2016 Jan;83(1):61-70.

Primary Rat Neurons Cells

(S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation

Zhang C, Zhang Z, Zhao Q, Wang X, Ji H, Zhang Y.

Neurotox Res , 26 (1), 16-31 Jul 2014

Primary Mouse Hippocampus & Cerebral Cortex Neurons Cells

Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons.

Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O.

J Neurosci. 2013 Jul 17;33(29):11932-48.

Primary Mouse CGE, MGE, & Cerebral Cortex Nerve Cells

Leucine-rich glioma inactivated 1 (Lgi1), an epilepsy-related secreted protein, has a nuclear localization signal and localizes to both the cytoplasm and the nucleus of the caudal ganglionic eminence neurons.

Kusuzawa S, Honda T, Fukata Y, Fukata M, Kanatani S, Tanaka DH, Nakajima K.

Eur J Neurosci. 2012 Aug;36(3):2284-92.

Transfection into Primary Cell Cultures by Electroporation

APPLICATIONS

Transfection Results with Nepa Gene Electroporation System

BMMC Primary Mouse Bone Marrow-Derived Mast cells

Primary Mouse Cerebral Cortex Neurons (E14)

Primary Human Endometrial Stromal Cells

Transfection Data: Primary Huyman Cells

Transfection Data: Primary Mouse Cells

Transfection Data: Primary Rat Cells

Transfection Data: Other Primary Cells

PUBLICATIONS

The Transcription Factor Ets1 Influences Axonal Growth via Regulation of Lcn2

Non-viral ex vivo genome-editing in mouse bona fide hematopoietic stem cells with CRISPR/Cas9

CXCR1 Expression to Improve Anti-Cancer Efficacy of Intravenously Injected CAR-NK Cells in Mice With Peritoneal Xenografts

CXCR4 Regulates Plasmodium Development in Mouse and Human Hepatocytes

Adoptive Transfer of NKG2D CAR mRNA-Engineered Natural Killer Cells in Colorectal Cancer Patients

Large-scale expansion of Vγ9Vδ2 T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor-modified effector cells.

OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex

Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia

Enhancement of antitumor activity by using a fully human gene encoding a single-chain fragmented antibody specific for carcinoembryonic antigen.

Endocytosis following dopamine D2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFRβ signaling in striatopallidal medium spiny neurons

A sister of NANOG regulates genes expressed in pre-implantation human development.

Targeting the rhesus macaque TRIM5α gene to enhance the susceptibility of CD4+ T cells to HIV-1 infection

Intraperitoneal immunotherapy with T cells stably and transiently expressing anti-EpCAM CAR in xenograft models of peritoneal carcinomatosis

A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury

Real-time imaging of mast cell degranulation in vitro and in vivo

Co-Expansion of Cytokine-Induced Killer Cells and Vγ9Vδ2 T Cells for CAR T-Cell Therapy

Expression of Channelrhodopsin-2 Using in Suspension Electroporation for Studying the Monosynaptic Transmission in Neuronal Culture

Evolutionary origin and functional divergence of totipotent cell homeobox genes in eutherian mammals

Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets

(S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation

Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons.

Leucine-rich glioma inactivated 1 (Lgi1), an epilepsy-related secreted protein, has a nuclear localization signal and localizes to both the cytoplasm and the nucleus of the caudal ganglionic eminence neurons.

Electroporation

■ Cell Cultures

■ In Vivo Mice/Rats

■ In Vivo Other Animals

■ Plant Cells & Algae

■ Exosomes

■ Bacteria, Yeast, Fungi

Drug Delivery and Transfection

■ Ultrasound Transfection and Drug Delivery (Sonoporation/Fus)

Electro Cell Fusion

■ Hybridoma Production

■ Oocyte Activation

■ Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer

■ Tetraploid Embryos Production

■ Other Applications

Fluorescent Staining

■ Autofluorescence Quenching

Single-Cell/Micro-Particle Transfer

■ Micro targets

■ Micro liquid

Cell Freezing

■ Cell Therapy

■ Animal Husbandry

Mechanical Vibration

■ Oocytes and Blastocysts