Transfection into Primary Cell Cultures by Electroporation

NEPA21

ELEPO21

Cuvette Chambers ＆ Stand Holder

Nepa Electroporation Cuvettes

Nepa Monitoring System

Transfection into Primary Cell Cultures by Electroporation

APPLICATIONS

Transfection Results with Nepa Gene Electroporation System

BMMC  Primary Mouse Bone Marrow-Derived Mast cells

Viability: 80%

Transfection Efficiency: 83%

FACS

bmmc_c-1

Primary Mouse Cerebral Cortex Neurons (E14)

Viability: 80％

Transfection Efficiency: 70％

Primary Human Endometrial Stromal Cells

Viability: 95％

Transfection Efficiency: 90％

Transfection Data: Primary Huyman Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
HUVEC Human Umbilical Vein Endothelial Cells95%75% HUVEC Human Umbilical Vein Endothelial Cells100%92%
HASM Human Airway Smooth Muscle Cells90%80% Human Coronary-derived Smooth Muscle Cells82%67%
Human Endometrial Stromal Cells95%90% Human Uterine Cervical Fibroblasts65%90%
Human Dermal Fibroblasts (HDF)95%89％ Human Dermal Fibroblasts (HDF)100%80%
Human Keratinocytes70%65% Human Malignant Mesothelioma Cells75％55％
Human Meniscal Cells85%55% Human Colorectal Cells53%80%
Human T Cells58%90% Human T Cells50%76%
Human NK Cells48%86% PBMC Peripheral Blood Mononuclear Cells93%66%
Human Erythroblasts (CD34+ Progenitor Cells)61％34％ Human Chronic Lymphocytic Leukemia (CLL)82%70%
Human Chronic Lymphocytic Leukemia (CLL) (mRNA)93％94% Human Chronic Lymphocytic Leukemia (CLL)91%83%
Human Chronic Lymphocytic Leukemia (CLL)83%93% Human Osteoblast-like Cells derived from human skull64%74%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Primary Mouse Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Mouse Cerebral Cortex Neurons (E14)80%70% Mouse Hippocampal Neurons (E14)80%60%
Mouse Hippocampal Neurons (E17)65%70% Mouse Neural Progenitor Cells80%60%
Mouse Basal Ganglia Primordium91%71% Mouse Cerebellar Granule Neurons91%65%
Mouse DRG Neurons83%68% Mouse DRG Neurons70%70%
MEF Mouse Embryonic Fibroblasts90%85% MEF Mouse Embryonic Fibroblasts75%85%
Mouse Embryonic Skin Fibroblasts80%50%    
Mouse External Genital Fibroblasts (E15.5)66%59% Mouse Cervical Epithelial Cells82%55%
Mouse Vascular Adventitial Fibroblasts90%50% BMMC Mouse Bone Marrow-Derived Mast Cells80%83%
Mouse peritoneal macrophages69%41% Mouse B cells (LPS stimulated)81%73%
Mouse B cells50%61% Mouse B cells (Unstimulated)84%83%
Mouse T cells (siRNA)88%77% Naive Mouse CD8+ T cells (Cas9 RNP)50%95%
Mouse Liver Cells75%65% Mouse Osteoblast Cells85%60%
Mouse Muscle Cells68%54%    

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Primary Rat Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Rat Cerebral Cortex Neurons (E16)70%75% Rat Hippocampal Neurons60%80%
Rat Bulbar Neurons80%75% Rat Cerebellar Neurons70%55%
Rat Cerebellar Granule Cells70%80% Rat Schwann Cells90%80%
Rat Schwann Cells90%60% OEC Rat Olfactory Ensheathing Cells93%46%
Rat Müller Cells90%50% REF Rat Embryonic Fibroblasts65%65%
Rat Meningeal Fibroblasts90%95% PASMC Rat Pulmonary Artery Smooth Muscle Cells72%70%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Other Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Chick Embryonic Fibroblasts80%90% Chick Embryonic Cerebellar Granule Cells86%83%
Bovine Cartilage Cells96%55% Goat Embryonic Epithelial Fibroblasts80%55%
Rabbit Spleen cells (B cells)70%45% Canine Adipose-Derived Stromal Cells (ADSCs) 65%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

  • Primary_chondrocytes

Cartilage stem/progenitor cells-derived exosomes facilitate knee cartilage repair in a subacute osteoarthritis rat model

Chen J, Ni X, Yang J, Yang H, Liu X, Chen M, Sun C, Wang Y.

J Cell Mol Med. 2024 Apr;28(8):e18327.

  • T_lymphocytes

CD3-activated T-lymphocytes

Differential impact of genetic deletion of TIGIT or PD-1 on melanoma-specific T-lymphocytes

Differential impact of genetic deletion of TIGIT or PD-1 on melanoma-specific T-lymphocytesCadiou G, Beauvais T, Marotte L, Lambot S, Deleine C, Vignes C, Gantier M, Hussong M, Rulli S, Jarry A, Simon S, Malissen B, Labarriere N.

Oncoimmunology. 2024 Jul 8;13(1):2376782.

  • BMDM

Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression

Jang HJ, Min HY, Kang YP, Boo HJ, Kim J, Ahn JH, Oh SH, Jung JH, Park CS, Park JS, Kim SY, Lee HY.

Nat Commun. 2024 Jun 8;15(1):4909.

  • Human_T_cells

ScRNA-seq reveals novel immune-suppressive T cells and investigates CMV-TCR-T cells cytotoxicity against GBM

Long X, Zhang Z, Li Y, Deng K, Gao W, Huang M, Wang X, Lin X, She X, Zhao Y, Zhang M, Huang C, Wang S, Du Y, Du P, Chen S, Liu Q, Wu M.

J Immunother Cancer. 2024 Apr 30;12(4):e008967.

  • Primary_rat_neuronal_cells

The Transcription Factor Ets1 Influences Axonal Growth via Regulation of Lcn2

Gu M, Li X, Wu R, Cheng X, Zhou S, Gu X.

Mol Neurobiol. 2024 Feb;61(2):971-981.

  • Human_T_cells

Epigenetic profiles guide improved CRISPR/Cas9-mediated gene knockout in human T cells

Ito Y, Inoue S, Nakashima T, Zhang H, Li Y, Kasuya H, Matsukawa T, Wu Z, Yoshikawa T, Kataoka M, Ishikawa T, Kagoya Y.

Nucleic Acids Res. 2024 Jan 11;52(1):141-153.

  • Human_monocytes

Systematic identification of gene combinations to target in innate immune cells to enhance T cell activation

Xia L, Komissarova A, Jacover A, Shovman Y, Arcila-Barrera S, Tornovsky-Babeay S, Jaya Prakashan MM, Nasereddin A, Plaschkes I, Nevo Y, Shiff I, Yosefov-Levi O, Izhiman T, Medvedev E, Eilon E, Wilensky A, Yona S, Parnas O. Xia L, Komissarova A, Jacover A, Shovman Y, Arcila-Barrera S, Tornovsky-Babeay S, Jaya Prakashan MM, Nasereddin A, Plaschkes I, Nevo Y, Shiff I, Yosefov-Levi O, Izhiman T, Medvedev E, Eilon E, Wilensky A, Yona S, Parnas O.

Nat Commun. 2023 Oct 9;14(1):6295.

  • Human_T_cells

Genetic ablation of PRDM1 in antitumor T cells enhances therapeutic efficacy of adoptive immunotherapy

Yoshikawa T, Wu Z, Inoue S, Kasuya H, Matsushita H, Takahashi Y, Kuroda H, Hosoda W, Suzuki S, Kagoya Y.

Blood. 2022 Apr 7;139(14):2156-2172.

  • Primary_mouse_hematopoietic_stem_cells_(HSCs)

Non-viral ex vivo genome-editing in mouse bona fide hematopoietic stem cells with CRISPR/Cas9

HoSuvd Byambaa, Hideki Uosaki, Tsukasa Ohmori, Hiromasa Hara, Hitoshi Endo, Osamu Nureki, Yutaka Hanazono

Mol Ther Methods Clin Dev. 2021 Jan 9:20:451-462.

    Primary Human NK Cells

    CXCR1 Expression to Improve Anti-Cancer Efficacy of Intravenously Injected CAR-NK Cells in Mice With Peritoneal Xenografts

    Yu Yang Ng, Johan C K Tay, Shu Wang

    Mol Ther Oncolytics. 2019 Dec 24:16:75-85.

      Primary Human Hepatocytes

      CXCR4 Regulates Plasmodium Development in Mouse and Human Hepatocytes

      Hironori Bando, Ariel Pradipta, Shiroh Iwanaga, Toru Okamoto, Daisuke Okuzaki, Shun Tanaka, Joel Vega-Rodríguez, Youngae Lee, Ji Su Ma, Naoya Sakaguchi, Akira Soga, Shinya Fukumoto, Miwa Sasai, Yoshiharu Matsuura, Masao Yuda, Marcelo Jacobs-Lorena, Masahiro Yamamoto

      J Exp Med. 2019 Aug 5;216(8):1733-1748.

        Primary Human NK Cells

        Adoptive Transfer of NKG2D CAR mRNA-Engineered Natural Killer Cells in Colorectal Cancer Patients

        Lin Xiao, Dongzhi Cen, Haining Gan, Yan Sun, Nanqi Huang, Hanzhen Xiong, Qiongmei Jin, Liqun Su, Xuejuan Liu, Kejian Wang, Guangrong Yan, Tianfa Dong, Shangbiao Wu, Pengzhi Zhou, Jinshan Zhang, Weixiang Liang, Junlan Ren, Yaoshu Teng, Can Chen, Xue Hu Xu

        Mol Ther, 27 (6), 1114-1125 2019 Jun 5

          Primary Human T cells

          Large-scale expansion of Vγ9Vδ2 T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor-modified effector cells.

          Xiao L, Chen C, Li Z, Zhu S, Tay JC, Zhang X, Zha S, Zeng J, Tan WK, Liu X, Chng WJ, Wang S

          Cytotherapy. 2018 Mar;20(3):420-435.

            Primary Mouse Neurons Cells

            OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex

            Huang B, Li X, Tu X, Zhao W, Zhu D, Feng Y, Si X, Chen JG

            J Biol Chem. 2018 Feb 9;293(6):2137-2148.

              Primary Human Skin Fibroblasts

              Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia

              Lesport E, Ferster A, Biver A, Roch B, Vasquez N, Jabado N, Vives FL, Revy P, Soulier J, de Villartay JP

              Oncotarget. 2017 Dec 17;9(3):3779-3793.

                PBMC and Primary Human T Cells

                Enhancement of antitumor activity by using a fully human gene encoding a single-chain fragmented antibody specific for carcinoembryonic antigen.

                Shibaguchi H, Luo N, Shirasu N, Kuroki M, Kuroki M

                Onco Targets Ther. 2017 Aug 22;10:3979-3990.

                  Primary Mouse Neurons Cells

                  Endocytosis following dopamine D2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFRβ signaling in striatopallidal medium spiny neurons

                  Shioda N, Yabuki Y, Wang Y, Uchigashima M, Hikida T, Sasaoka T, Mori H, Watanabe M, Sasahara M, Fukunaga K

                  Mol Psychiatry. 2017 Aug;22(8):1205-1222.

                    Primary Human Skin Fibroblasts

                    A sister of NANOG regulates genes expressed in pre-implantation human development.

                    Dunwell TL, Holland PWH

                    Open Biol. 2017 Apr;7(4). pii: 170027.

                      Primary Rhesus Macaque CD4+ T cells

                      Targeting the rhesus macaque TRIM5α gene to enhance the susceptibility of CD4+ T cells to HIV-1 infection

                      Wang X, Yu Q, Yuan Y, Teng Z3, Li D, Zeng Y

                      Arch Virol. 2017 Mar;162(3):793-798.

                        Primary Human T cells

                        Intraperitoneal immunotherapy with T cells stably and transiently expressing anti-EpCAM CAR in xenograft models of peritoneal carcinomatosis

                        Ang WX, Li Z, Chi Z, Du SH, Chen C, Tay JC1, Toh HC, Connolly JE, Xu XH, Wang S

                        Oncotarget. 2017 Feb 21;8(8):13545-13559.

                        • Primary_Mouse_Astrocytes

                        Primary Mouse Astrocytes

                        A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury

                        Ishii T, Ueyama T, Shigyo M, Kohta M, Kondoh T, Kuboyama T, Uebi T, Hamada T, Gutmann DH, Aiba A, Kohmura E, Tohda C, Saito N

                        J Biol Chem. 2017 Jan 27;292(4):1240-1250.

                        • BMMCs

                        Primary Mouse Bone Marrow-Derived Mast cells (BMMCs)

                        Real-time imaging of mast cell degranulation in vitro and in vivo

                        Horiguchi K, Yoshikawa S, Saito A, Haddad S, Ohta T, Miyake K, Yamanishi Y, Karasuyama H

                        Biochem Biophys Res Commun. 2016 Oct 21;479(3):517-522.

                          Primary Human T cells

                          Co-Expansion of Cytokine-Induced Killer Cells and Vγ9Vδ2 T Cells for CAR T-Cell Therapy

                          Du SH, Li Z, Chen C, Tan WK, Chi Z, Kwang TW, Xu XH, Wang S

                          PLoS One. 2016 Sep 6;11(9):e0161820.

                            Primary Rat Neurons Cells

                            Expression of Channelrhodopsin-2 Using in Suspension Electroporation for Studying the Monosynaptic Transmission in Neuronal Culture

                            Natalia Bal, Aleksey Malyshev, Ivan Smirnov, Pavel Balaban

                            BioNanoScience 6, 329–331(2016)

                              Primary Human Skin Fibroblasts

                              Evolutionary origin and functional divergence of totipotent cell homeobox genes in eutherian mammals

                              Maeso I, Dunwell TL, Wyatt CD, Marlétaz F, Vető B, Bernal JA, Quah S, Irimia M, Holland PW

                              BMC Biol. 2016 Jun 13;14:45.

                                Primary Porcine Dermal Fibroblasts, Kidney Fibroblasts, and Myoblasts

                                Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets

                                Rao S, Fujimura T, Matsunari H, Sakuma T, Nakano K, Watanabe M, Asano Y, Kitagawa E, Yamamoto T, Nagashima H

                                Mol Reprod Dev. 2016 Jan;83(1):61-70.

                                  Primary Rat Neurons Cells

                                  (S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation

                                  Zhang C, Zhang Z, Zhao Q, Wang X, Ji H, Zhang Y.

                                  Neurotox Res , 26 (1), 16-31 Jul 2014

                                  • hMSCs

                                  Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells

                                  Aomatsu E, Takahashi N, Sawada S, Okubo N, Hasegawa T, Taira M, Miura H, Ishisaki A, Chosa N.

                                  Sci Rep. 2014 Jan 13:4:3652.

                                    Primary Mouse Hippocampus & Cerebral Cortex Neurons Cells

                                    Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons.

                                    Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O.

                                    J Neurosci. 2013 Jul 17;33(29):11932-48.

                                      Primary Mouse CGE, MGE, & Cerebral Cortex Nerve Cells

                                      Leucine-rich glioma inactivated 1 (Lgi1), an epilepsy-related secreted protein, has a nuclear localization signal and localizes to both the cytoplasm and the nucleus of the caudal ganglionic eminence neurons.

                                      Kusuzawa S, Honda T, Fukata Y, Fukata M, Kanatani S, Tanaka DH, Nakajima K.

                                      Eur J Neurosci. 2012 Aug;36(3):2284-92.

                                      Electroporation

                                      Drug Delivery and Transfection

                                      Electro Cell Fusion

                                      Fluorescent Staining

                                      Single-Cell/Micro-Particle Transfer

                                      Cell Freezing

                                      Mechanical Vibration