Transfection into Mouse/Rat Retina/Cornea/Spinal Cord/Sciatic Nerve by Electroporation

NEPA21

Tweezer Electrodes

Cables, Foot Switch & Others

Transfection into Mouse/Rat Retina/Cornea/Spinal Cord/Sciatic Nerve by Electroporation

APPLICATIONS

Transfection into Mouse/Rat Retina by Electroporation

Schematic illustration showing the in vivo electroporation method.
(1). DNA injection into subretinal space
(2). Electroporation

Tweezer-type electrodes (3) are placed to hold the head of newborn (P0) rat or mouse (4).

In vivo electroporated rat retinae harvested at various developmental stages.

 

(a) Whole-mount preparation of rat retina in vivo electroporated with pCAG-GFP at P0 and harvested at P21. Pictures were taken from the scleral side.

 

(b) Rat retinae in vivo electroporated with pCAG-GFP at P0 were harvested at P2(d-f ), or P5(g-i), and cryosections were prepared.

 

Takahiko Matsuda, Cepko laboratory, Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School

PUBLICATIONS

    In vivo Retina

    dCasMINI-mediated therapy rescues photoreceptors degeneration in a mouse model of retinitis pigmentosa

    Wang Q, Xu X, Chen S, Lu R, Li L, Lo CH, Liu Z, Ning K, Li T, Kowal TJ, Wang B, Hartnett ME, Wang S, Qi LS, Sun Y.

    Sci Adv. 2024 Dec 20;10(51):eadn7540.

      In vivo Retina

      Optimization of HITI-Mediated Gene Insertion for Rhodopsin and Peripherin-2 in Mouse Rod Photoreceptors: Targeting Dominant Retinitis Pigmentosa

      Onishi A, Tsunekawa Y, Mandai M, Ishimaru A, Ohigashi Y, Sho J, Yasuda K, Suzuki K, Izpisua Belmonte JC, Matsuzaki F, Takahashi M.

      Invest Ophthalmol Vis Sci. 2024 Nov 18;65(13):38.

        Ex vivo Spinal cord

        Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation

        Kinoshita-Kawada M, Hasegawa H, Hongu T, Yanagi S, Kanaho Y, Masai I, Mishima T, Chen X, Tsuboi Y, Rao Y, Yuasa-Kawada J, Wu JY.

        Bio Protoc. 2019 Sep 20;9(18):e3373.

          In vivo Retina

          SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation

          Ros O, Zagar Y, Ribes S, Baudet S, Loulier K, Couvet S, Ladarre D, Aghaie A, Louail A, Petit C, Mechulam Y, Lenkei Z, Nicol X.

          Cell Rep. 2019 Jun 25;27(13):4003-4012.e6.

            Ex vivo Retina

            Quantitative Analysis of the ThrbCRM1-centered Gene Regulatory Network

            Benjamin Souferi, Mark M Emerson

            Biol Open . 2019 Apr 26;8(4):bio039115.

              In vivo Spinal cord

              Convallatoxin Enhance the Ligand-Induced Mu-Opioid Receptor Endocytosis and Attenuate Morphine Antinociceptive Tolerance in Mice

              Chao PK, Chang HF, Ou LC, Chuang JY, Lee PT, Chang WT, Chen SC, Ueng SH, Hsu JT, Tao PL, Law PY, Loh HH, Yeh SH.

              Sci Rep. 2019 Feb 20;9(1):2405.

                In vivo Sciatic Nerve

                Molecular analysis of axonal-intrinsic and glial-associated co-regulation of axon degeneration

                Catenaccio A, Llavero Hurtado M, Diaz P, Lamont DJ, Wishart TM, Court FA.

                Cell Death Dis. 2017 Nov 9;8(11):e3166

                  Ex vivo Spinal cord

                  A crucial role for Arf6 in the response of commissural axons to Slit

                  Kinoshita-Kawada M, Hasegawa H, Hongu T, Yanagi S, Kanaho Y, Masai I, Mishima T, Chen X, Tsuboi Y, Rao Y, Yuasa-Kawada J, Wu JY

                  Development. 2019 Feb 4;146(3).

                    In vivo/Ex vivo Retina

                    Gene Transfer of Prolyl Hydroxylase Domain 2 Inhibits Hypoxia-inducible Angiogenesis in a Model of Choroidal Neovascularization.

                    Takei A, Ekström M, Mammadzada P, Aronsson M, Yu M, Kvanta A, André H

                    Sci Rep. 2017 Feb 10;7:42546.

                      In vivo Spinal cord

                      Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling.

                      Lee PT, Chao PK, Ou LC, Chuang JY, Lin YC, Chen SC, Chang HF, Law PY, Loh HH, Chao YS, Su TP, Yeh SH

                      Nucleic Acids Res. 2014 Dec 1;42(21):13012-25.

                        In vivo Sciatic nerve

                        Schwann cell-derived exosomes enhance axonal regeneration in the peripheral nervous system.

                        Lopez-Verrilli MA, Picou F, Court FA.

                        Glia. 2013 Nov;61(11):1795-806.

                          In vivo/Ex vivo Retina

                          Requirement of retinoic acid receptor β for genipin derivative-induced optic nerve regeneration in adult rat retina.

                          Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Sugitani K, Arai K, Suzuki H, Kato S.

                          PLoS One. 2013 Aug 6;8(8):e71252.

                            In vivo Spinal cord

                            CCL-1 in the spinal cord contributes to neuropathic pain induced by nerve injury.

                            Akimoto N, Honda K, Uta D, Beppu K, Ushijima Y, Matsuzaki Y, Nakashima S, Kido MA, Imoto K, Takano Y, Noda M.

                            Cell Death Dis. 2013 Jun 20;4(6):e679.

                              Ex vivo Retina

                              Identification of a Retina-Specific Otx2 Enhancer Element Active in Immature Developing Photoreceptors

                              Mark M Emerson, Constance L Cepko

                              Dev Biol, 360 (1), 241-55 2011 Dec 1

                                Ex vivo Retina

                                Bulk electroporation and population calcium imaging in the adult mammalian retina.

                                Briggman KL, Euler T.

                                J Neurophysiol. 2011 May;105(5):2601-9.

                                  Ex vivo Retina

                                  Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.

                                  Ozawa Y et al.

                                  J Biol Chem. 2008 Sep 5;283(36):24561-70.

                                    In vivo/Ex vivo Retina

                                    A role for ligand-gated ion channels in rod photoreceptor development

                                    Young TL, Cepko CL.

                                    Neuron. 2004 Mar 25;41(6):867-79.

                                      In vivo/Ex vivo Retina

                                      Electroporation and RNA interference in the rodent retina in vivo and in vitro

                                      Matsuda T, Cepko CL.

                                      Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):16-22.

                                        In vivo/Ex vivo Cornea

                                        Targeted gene transfer to corneal stroma in vivo by electric pulses

                                        Oshima Y, Sakamoto T, Hisatomi T, Tsutsumi C, Sassa Y, Ishibashi T, Inomata H.

                                        Exp Eye Res . 2002 Feb;74(2):191-8.

                                          In vivo/Ex vivo Retina

                                          Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach

                                          Dezawa M, Takano M, Negishi H, Mo X, Oshitari T, Sawada H.

                                          Micron. 2002;33(1):1-6.

                                          Electroporation

                                          Drug Delivery and Transfection

                                          Electro Cell Fusion

                                          Fluorescence Quenching / in situ Hybridization Chain Reaction

                                          Single-Cell/Micro-Particle Transfer

                                          Cell Freezing

                                          Mechanical Vibration