Transfection into Cell Lines by Electroporation

The Nepa Gene electroporators make it possible to achieve high transfection efficiency and high viability without resourse to special buffers. It works not only for plasmid DNA but also for siRNA, mRNA, shRNA, proteins and more.

Transfection into Cell Lines by Electroporation

APPLICATIONS

Transfection into Cell Line

293T(HEK293T): Human Embryonic Kidney Cells

Viabiliy:83%

Transfection Effeciency:87%

Jurkat: Human T-cell Leukemia Cells

Viability:73%

Transfection Efficiency:94%

Neuro-2a: Mouse Neuroblastoma Cells

Viability:90%

Transfection Efficiency:90%

Transfection into Cell Lines

Species: Human

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell Lines V TE   Cell Lines V TE
HeLa Human Cervical Carcinoma Cells 87% 93%   HeLa-K Human Cervical Carcinoma Cells 90% 90%
293 Human Embryonic Kidney Cells 92% 91%   293 Human Embryonic Kidney Cells 90% 70%
293 Human Embryonic Kidney Cells 72% 85%   293T Human Embryonic Kidney Cells 90% 95%
293T Human Embryonic Kidney Cells 83% 87%   293T Human Embryonic Kidney Cells 70% 99%
TIG-3 Human Embryonic Lung Fibroblasts 90% 80%   TIG-7 Human Embryonic Lung Fibroblasts 89% 76%
MRC-5 Human Embryonic Lung Fibroblasts 85% 90%   WI-38 Human Embryonic Lung Fibroblasts 80% 70%
CCD18-Co Human Colon Fibroblasts 99% 61%   HaCat Human Keratinocyte Cells 96% 69%
HDF Human Dermal Fibroblasts (106-05) 90% 90%   TIG-109 Human Skin Fibroblasts 70% 60%
HFE145 Human Non-Cancerous Gastric Epithelial Cells 80% 50%   BEAS-2B Human Bronchial Epithelial Cells 75% 96%
BEAS-2B Human Bronchial Epithelial Cells 90% 80%   KMST-6 Human Fibroblasts 70% 60%
SUSM-1 Human Fibroblasts 77% 71%   HT1080 Human Fibrosarcoma Cells 93% 81%
SEKI Human Malignant Melanoma Cells  80% 75%   MG-63 Human Osteosarcoma Cells 70% 80%
HT1080 Human Fibrosarcoma Cells 80% 90%   Saos-2 Human Osteosarcoma Cells 60% 75%
U2OS Human Osteosarcoma Cells 70% 80%   PANC-1 Human Pancreatic Carcinoma Cells 78% 70%
Saos-2 Human Osteosarcoma Cells 80% 70%   MIA-PaCa-2 Human Pancreatic Carcinoma Cells 80% 77%
PANC-1 Human Pancreatic Carcinoma Cells 55% 75%   HuH-7 Human Hepatoma Cells 90% 86%
HepG2 Human Hepatoma Cells 88% 76%   HLF Human Liver Cancer Cells 90% 85%
HuH-7 Human Hepatoma Cells 82% 85%   H69 Human Small-Cell Lung Cancer Cells 90% 85%
TFK-1 Human Bile Duct Adenocarcinoma Cells 50% 70%   LC319 Human Lung Cancer Cells 95% 66%
NCI-H69 Human Small-Cell Lung Cancer Cells 97% 70%   H1299 Human Lung Cancer Cells 80% 80%
H1299 Human Lung Cancer Cells 90% 90%   HSC-2 Human Squamous Carcinoma Cells 93% 98%
KB31 Human Epidermoid Carcinoma Cells 58% 64%   Ca9-22 Human Squamous Carcinoma Cells 60% 60%
HSC-3 Human Squamous Carcinoma Cells 93% 98%   HEp-2 Human Laryngeal Carcinoma Cells 70% 90%
HGF Human Gingival Fibroblasts       MCF-7 Human Breast Cancer Cells 95% 80%
MCF-7 Human Breast Cancer Cells 81% 65%   T47D Human Breast Cancer Cells 90% 85%
MCF-7 Human Breast Cancer Cells 80% 70%   MDA-MB-231 Human Breast Cancer Cells 85% 90%
BT-20 Human Breast Cancer Cells 70% 80%   MCF 10A Human Breast Cells    
MCF 10A Human Breast Cells 90% 80%   A549 Human Lung Adenocarcinoma Cells 85% 90%
MCF 10A Human Breast Cells 97% 69%   GC38 Human Gastric Cancer Cells 80% 80%
NUGC-3 Human Gastric Carcinoma Cells 73% 68%   Human Patient-Derived Gastric Cancer Cells 44% 74%
MKN-45 Human Gastric Cancer Cells  78% 73%        
LNCaP Human Prostate Carcinoma 71%  90%   PC-3 Human Prostate Cancer Cells 90% 95%
DU145 Human Prostate Cancer Cells 94% 60%   PNT2 Human Prostate Epithelial Cells 85%  80%
PC-3 Human prostate Cancer Cells 86% 55%   HCT116 Human Colon Cancer Cells  80% 95%
Lovo Human Colon Adenocarcinoma Cells 85%  60%   HCT116 Human Colon Cancer Cells 95% 90%
HCT116 Human Colon Cancer Cells  80% 80%   Caco-2 Human Colon Cancer Cells 85% 80%
Caco-2 Human Colon Cancer Cells 95% 80%   OVCAR-3 Human Colon Cancer Cells 90% 79%
SW620 Human Colon Cancer Cells 80% 80%   RMG-1 Human Ovarian Clear Cell Adenocarcinoma 97% 67%
SKOV-3 Human Ovarian Carcinoma Cells 90% 90%   SH-SY5Y Human Neuroblastoma Cells 60% 90%
SK-N-SH Human Neuroblastoma Cells 95% 95%   SH-SY5Y Human Neuroblastoma Cells 79% 60%
SH-SY5Y Human Neuroblastoma Cells 70% 70%   NB69 Human Neuroblastoma Cells 95% 80%
NB9 Human Neuroblastoma Cells 70% 70%   KG-1-C Human Oligodendroglial Cells 85% 60%
NB-39-nu Human Neuroblastoma Cells 60% 63%   NP3 Human Glioblastoma Cells 98%  62%
A172 Human Glioblastoma Cells  85% 70%   1321N1 Human Astrocytoma Cells 80% 80%
U87 MG Human Glioblastoma/Astrocytoma Cells 70% 55%   Immortalized Human Pericytes 83% 50%
U-251 Human Glioblastoma Cells 90% 60%   Human Dental Pulp Cells 85% 69%
iHAM-4 Human Amniotic Mesenchymal Cells 59% 95%   HTR-8/Svneo Human Trophoblast Cells 95% 67%
Human Dental Pulp Cells 90% 85%   RPE Retinal Pigment Epithelium Cells 90% 70%
SRA 01/04 Human Lens Epithelial Cells 97% 80%   RPTEC Human Renal Proximal Tubule Epithelial Cells 70% 85%
RPE-1 Retinal Pigment Epithelium Cells 95% 80%   Jurkat Human T-cell Leukemia Cells 73% 94%
HK-2 Human Renal Proximal Tubule Epithelial Cells 50% 90%   Jurkat Human T-cell Leukemia Cells 89% 85%
Jurkat Human T-cell Leukemia Cells 85% 85%   ED40515 Human T-cell Leukemia Cells 82% 84%
Jurkat Human T-cell Leukemia Cells 99% 92%   Hut78 Human T-cell Lymphoma Cells 51% 74%
SNT16 Human T-cell Lymphoma Cells  85% 84%   Jeko-1 Human Mantle Cell Lymphoma (MCL) Cells  80% 63%
Jeko-1 Human Mantle Cell Lymphoma (MCL) Cells 82% 71%   MV4-11 Human Acute Myeloid Leukemia Cells 70%  60%
MOLT-4 Human Acute Lymphoblastic Leukemia Cells 95% 70%   MEC1 Human Chronic Lymphocytic Leukemia Cells >90% >90%
697 Human Pre-B Acute Lymphoblastic Leukemia Cells 68% 93%   Nalm-6 Human B-cell Precursor Leukemia Cells 97% 76%
Nalm-6 Human B-cell Precursor Leukemia Cells 77% 82%   KG-1 Human Acute Myeloid Leukemia Cells 70%  65%
KG-1 Human Acute Myeloid Leukemia Cells 60% 65%   PL-21 Human Acute Myeloid Leukemia Cells 51% 73%
MOLM-16 Human Acute Myeloid Leukemia Cells 74% 68%   USCD/AML1 Human Leukemia Cells 50% 50%
Kasumi-1 Human Acute Myeloid Leukemia Cells 66% 79%   KOPT-K1 Human T cell Acute Lymphoblastic Leukemia (T-ALL) Cells 80% 60%
M7 Human Acute Non Lymphocytic Leukemia 85% 80%   GM12878 Human B-Lymphoblastoid Cells 93% 83%
Loucy Human T cell Acute Lymphoblastic Leukemia (T-ALL) Cells 73% 50%   Human EBV-immortalized B Cells 58% 53%
T2 Human T and B lymphoblast Cells 97% 97%   Raji Human Burkitt’s Lymphoma Cells 97% 83%
Namalwa Human Burkitt’s Lymphoma Cells 70% 75%   Raji Human Burkitt’s Lymphoma Cells 85% 79%
Raji Human Burkitt’s Lymphoma Cells 95% 96%   SU-DHL-4 Human Burkitt’s Lymphoma Cells 79% 68%
Toledo Human Burkitt’s Lymphoma Cells 81% 60%        
SU-DHL-10 Human B-cell Lymphoma Cells 78% 68%   K562 Human Chronic Myelogenous Leukemia Cells 91% 99%
eHAP1 Human Haploid Cells 72% 65%   K562 Human Chronic Myelogenous Leukemia Cells >90% 94%
K562 Human Chronic Myelogenous Leukemia Cells >90% >90%   HL-60 Human Promyelocytic Leukemia Cells 80% 80%
HL-60 Human Promyelocytic Leukemia Cells 81% 82%   Mutu Ⅰ Human Burkitt Lymphoma Cells 87% 91%
PLB-985 Human Myeloid Leukemia Cells 94% 92%   Ramos Human Burkitt Lymphoma Cells 92% 57%
Mutu III Human Burkitt Lymphoma Cells 54% 92%   Ramos-Blue Human Burkitt Lymphoma Cells 80% 55%
Ramos Human Burkitt Lymphoma Cells 83% 57%   BJAB Human EBV-negative Burkitt Lymphoma Cells 96% 96%
Z-138 Human Mantle Cell Lymphoma Cells 93% 86%   TK6 Human B-Lymphoblast Cells 84% 79%
SKM-1 Human MDS-derived Leukaemia Cells 88% 83%   THP-1 Human Acute Monocytic Leukemia Cells 56% 64%
THP-1 Human Acute Monocytic Leukemia Cells 76% 63%   THP-1 Human Acute Monocytic Leukemia Cells  67% 85%
THP-1 Human Acute Monocytic Leukemia Cells 85% 67%   NK-92MI Human Natural Killer (NK) Cells 83% 95%
HMC1.2 Human Mast Leukemia Cells 77% 89%   MTA Human Natural Killer-Like Leukemia Cells 65% 61%
KHYG-1 Human Natural Killer (NK)Lleukemia Cells 51% 71%        

 

 

Species: Mouse

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell Lines V TE   Cell Lines V TE
NIH/3T3 Mouse Embryonic Fibroblasts 92% 91%   NIH/3T3 Mouse Embryonic Fibroblasts 90% 70%
PT67 Mouse Fibroblasts (RetroPack PT67 cell line)       3T3-L1 Mouse Embryonic Fibroblasts (preadipocytes) 90% 90%
MEF Mouse Embryonic Fibroblasts 90% 90%   MEF Mouse Embryonic Fibroblasts 80% 90%
STO Mouse Embryonic Fibroblasts 60% 51%   N7 Mouse Embryonic Hypothalamic cells (immortalized) 75%  100%
P19C6 Mouse Embryonic Carcinoma Cells 90% 50%   F9 Mouse Testis Teratocarcinoma Cells 85% 95%
HL-1 Mouse Cardiac Muscle Cells 70% 70%   L Mouse Fibroblasts 90% 65%
B16 Mouse Melanoma Cells 86% 76%   B16 Mouse Melanoma Cells 77% 83%
B16 Mouse Melanoma Cells 70% 50%   MC3T3-E1 Mouse Osteoblastic Cells 85% 75%
C2C12 Mouse Myoblast Cells 94% 90%   C2C12 Mouse Myoblast Cells  90% 90%
C2C12 Mouse Myoblast Cells 80% 70%   C2C12 Mouse Myoblast Cells 94% 96%
bEnd.3 Mouse Brain Endothelial Cells 80% 80%   NMuMG Mouse Mammary Gland Epithelial Cells 80%  65%
Mouse Podocytes (Kidney Epithelial cells) 100% 84%   Mouse Podocytes (Kidney Epithelial cells) 66% 68%
LLc1(LL/2) Lewis Lung Cell Carcinoma 1 Cells 87% 81%   4T1 Mouse Breast Cancer Cells 90% 95%
FM3A Mouse Breast Cancer Cells 77% 57%   3134 Mouse Mammary Adenocarcinoma Cells 100% 70%
Ehrlich Mouse Ehrlich-Lettre Ascites Carcinoma Cells 76% 68%   Colon-26 Mouse Colon Adenocarcinoma Cells 95%  90%
Hepa1-6 Mouse Hepatoma Cells 50% 98%   LM8 Mouse Osteosarcoma Cells 90% 85%
S180 Mouse Sarcoma Cells 72% 57%   MS-1 Mouse Pancreatic Endothelial Cells 90% 90%
ddy Mouse Endometrial Cells 60%  80%   ID-8 Mouse Ovarian Cancer Cells  95% 99%
AtT-20 Mouse Pituitary Tumor Cells 80% 80%   TtT/GF Mouse Pituitary Folliculo-Stellate-Like Cells 65% 83%
Neuro-2a Mouse Neuroblastoma Cells 90% 90%   GL261 E9 Mouse Glioma Cells 55% 57%
BV-2 Mouse Microglial Cells 92% 70%   BV-2 Mouse Microglial Cells 90% 50%
BV-2 Mouse Microglial Cells 80% 80%        
mDP Mouse Dental Pupilla Cells 65% 70%   MEL Mouse Erythroleukemia Cells 70% 50%
L1210 Mouse Lymphocytic Leukemia Cells 85% 70%   WR19L Mouse T-cell lymphoma Cells 92% 60%
EL4 Mouse T-cell lymphoma Cells 87% 82%   BA/F3 Mouse pro-B Cells 91% 92%
BA/F3 Mouse pro-B Cells 90% 90%   A20 Mouse B-cell Lymphoma Cells 99%  85%
A20 Mouse B-cell Lymphoma Cells 70% 65%   CH12F3 Mouse B Lymphoma Cells 74% 77%
WEHI-231 Mouse B-cell Lymphoma Cells 98% 73%   P815 Mouse Mastocytoma Cells  67% 68%
J774.1 Mouse Macrophage-like Cells 100% 70%   RAW264.7 Mouse Macrophage-like Cells 70% 56%
RAW264.7 Mouse Macrophage-like Cells       RAW264.7 Mouse Macrophage-like Cells 70% 70%
RAW-D Mouse Macrophage-like Cells 80% 80%   MIN6 Mouse Pancreatic Beta Cells 57% 71%
DC2.4 Mouse Dendritic Cells 42% 66%   XS106 Mouse Dendritic Cells 61% 45%
mDC Mouse Myeloid Dendritic Cells 79% 72%   416B Mouse Primitive Myeloid Cells 89% 64%
32D Mouse Myeloid Cells   88%   MLO-Y4 Mouse Osteocyte-like cells 99% 59%
MC/9 Mouse Mast Cells 76% 84%   MC/9 Mouse Mast Cells 87% 89%
BMBa Mouse Bone marrow-derived basophils 45% 67%   TS Mouse Trophoblast Stem Cells 59% 47%
Mouse hybridoma cells (lymphocytes and myeloma cells) 100% 66%  

Mouse T cell hybridoma cells
Courtesy of Prof. Yokosuka and Dr. Wakamatsu, Department of Immunology, Tokyo Medical University

69% 90%

 

 

Species: Rat

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell Lines V TE   Cell Lines V TE
PC12 Rat Adrenal Pheochromocytoma Cells 90% 70%   H9c2 Rat Ventricular Myoblasts 71% 82%
H9c2 Rat Ventricular Myoblasts 75% 80%   REF Rat Embryonic Fibroblasts 90% 99%
RSC96 Rat Schwann Cells 70% 85%   A7r5 Rat Aortic Smooth Muscle Cells 93% 75%
C6 Rat Glioma Cells 80% 67%   UMR106 Rat Osteoblastic Cells 80% 70%
RCS Rat Chondrosarcoma Cells 75% 50%   SF2 Rat Dental Epithelial Cells 80% 90%
HAT-7 Rat Dental Epithelial Cells 80% 90%        

 

 

Spiecies: Chinese Hamster

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell Lines V TE   Cell Lines V TE
CHO Chinese Hamster Ovary Cells  74% 90%   CHO Chinese Hamster Ovary Cells 98% 87%
CHO Chinese Hamster Ovary Cells 97% 97%   CHO-DG44 Chinese Hamster Ovary Cells 86% 80%
CHO-K1 Chinese Hamster Ovary Cells 95% 95%   CHO-K1 Chinese Hamster Ovary Cells 90% 99%
CHO-S Chinese Hamster Ovary Cells 94% 93%        

 

 

Other Species

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell Lines V TE   Cell Lines V TE
COS-7 African Green Monkey Kidney fibroblasts 61% 89%    Vero African Green Monkey Kidney Epithelial Cells 85% 85%
MDCK Madrin-Darby Canine Kidney Cells 90% 95%   MDCK Madrin-Darby Canine Kidney Cells  91% 80%
BFF Bovine Fetal Fibroblasts 93% 71%   BFF Bovine Fetal Fibroblasts 78% 72%
CKT-1 Bovine Kidney Epithelial Cells 75% 75%   Bovine Fibroblasts 90% 63%
BAEC Bovine Aortic Endothelial Cells 80% 80%   LLC-PK1 Pig Kidney Epithelial Cells 80% 85%
CPK Porcine Kidny Cells 93% 60%   PGCs Chicken Primordial Germ Cells 98% 63%
DT40 Chichken B Cells 72% 85%   DT40 Chichken B Cells 71% 60%
A6 Xenopus Kidney Epithelial Cells 90% 60%   Exosomes (labeled DNA oligos)    

We have a lot of data of cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration