Transfection into Organoids by Electroporation

Transfection into Organoids by Electroporation

APPLICATIONS

Production of genetically modified organoids by electroporation

After electroporation, TP53 knockout colon organoids (TP53 KO) were obtained by selection with Nutlin-3, a niche factor for TP53.
The TP53 KO and the normal colon organoid without TP53 mutation (WT) were cultured in Nutlin-3- and Nutlin-3-free medium for 7 days, and the TP53 KO was not affected by Nutlin-3 addition because it had acquired Nutlin-3 tolerance, while the WT did not grow under Nutlin-3-supplemented conditions.

Courtesy of Professor Toshiro Sato, Dr. Mami Matano, Dr. Mariko Shimokawa, Dr. Masayuki Fujii, School of Medicine, The Sakaguchi Laboratory, Department of Organoid Medicine, Keio University

 

Transfection into organoids

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cells name V TE   Cells name V TE
Human Normal Fundic Gastric Organoids   68%   Mouse Fundic Gastric Organoids   65%
Mouse Colorectal Cancer Organoids 100% 50%   Mouse Small Intestine Organoids    

We have a lot of data of organoids transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration