Super Electroporator NEPA21 Type II
In Vitro & In Vivo Electroporation
- High transfection efficiency & viability WITHOUT special buffers
- For difficult-to-transfect cells such as primary cells, stem cells, immune cells, blood cells, and more
- Transfection even into cells IN ADHERENCE
- Ideal for CRISPR/Cas9 genome editing
APPLICATIONS
Electroporation
- Zygotes In Vitro (TAKE method)
- Zygotes In Oviduct (i-GONAD method)
- Embryos In Utero
- Ex Utero Embryos In Vitro
- Brain
- Retina / Cornea / Spinal Cord / Sciatic Nerve
- Lung / Spleen / Liver / Stomach/ Kidney / Intestine
- Pancreas / Islets of langerhans
- Testis / Ovary / Prostate / Gonad / Uterus
- Muscle / Skin / Joint / Cartilage / Tumor / Others
PRODUCT DESCRIPTION
NEPA21 Novel 4-Step Multiple Electroporation Pulsing
NEPA21 pulse waveform（x: time y: voltage）
The novel 4-step pulsing with voltage decay results in higher transfection efficiency and higher viability WITHOUT special buffers.
- Poring Pulse Mode:
- Higher voltage, shorter duration, multiple pulses, voltage decay; This is for forming pores (small holes) in cell membrane with minimum damage.
- Polarity Exchanged Poring Pulse
- This can be applied to tissue transfection as well.
- Transfer Pulse Mode:
- Lower voltage, longer duration, multiple pulses, voltage dcay; This is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum damage.
- Polarity Exchanged Transfer Pulse
- This can increase the transfection efficiency.
Compare with other companies' devices
Is your lab still using a transfection device that requires expensive disposable kits?
We hear from many researchers that they are not happy with how much it costs to run.
| Transfection Device | Characteristics | Consumables |
| NEPA21 (Nepa Gene) | New electroporation without Special Buffers | Economy cuvettes USD 2.60/reaction |
| N (Company L) | Electroporation with special buffers | Special kits USD 25.00/reaction |
| N (Company T) | Electroporation with special tips | Special kits USD 21.00/reaction |
The running costs of the NEPA21 is much lower than other transfection devices!
Numerous achievements: in vivo electroporation, zygote electroporation for genome editing and more
NEPA21 can be used not only for cell culture electroporation, but also for many in vivo gene transfer applications in animals such as mice and rats, including zygote electroporation for the production of genetically modified organisms (GMOs).
For more details, please click on the target application at the top of this page or contact us.
Accurate and precise measurement of sample electrical resistance and electroporation pulse voltage, current, and power
In the electrical pulses of electroporation, the parameters of Ohm’s law – “voltage”, “current” & “resistance” – have a great influence on the experimental results. The reproducibility of the same “voltage”, “current” & “resistance” for each electroporation is important to achieve stable gene transfer results. Since the NEPA21 can measure the resistance, output the voltage and measure the current with great accuracy, it can control these factors.
The NEPA21 also provides a measure of electrical pulse power in joule.
By knowing more about the electrical parameters of electroporation, it is now possible to achieve more optimal electrical conditions for each application.
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Note: The specifications and appearance of products on this page are subject to change without notice.