Other Applications using Electrical Cell Fusion

ECFG21

Petridish/Microscope Slide Electrodes

Cables, Foot Switch & Others

Nepa Monitoring System

Other Applications using Electrical Cell Fusion

APPLICATIONS

ECFG21 Liposome/droplet Fusion

Timing controllable electrofusion device for aqueous droplet-based microreactors

Fig. 1. Electrofusion of droplets in the fusion chamber.
(a) Droplets formed upstream enter the fusion chamber.
(b) Due to the widening of the chamber, the droplets slow down and make contact when it enters the fusion chamber.
(c) Upon the application of an electric field (50V, 750m gap, Pulse width: 10s, Interval: 0.2sec, 5 times) the contacting droplets coalesce.
(d) Photo showing two coalesced droplets. *The round particle near the top electrode was an air bubble.
Electric pulses were applied with an Electro Cell Fusion Unit (LF101, NEPA GENE).

 

 

Fig. 2. High speed camera images of the fusion process.
This fusion process is almost instantaneous. The two droplets combined into one single ”peanut-shaped” droplet within about 1ms. It took about another 5ms for the droplet to adopt a spherical shape under the effect of surface tension. Throughout the fusion process, the darker colored blue ink droplet (leftmost) was distinctly separated from the lighter colored water droplet (rightmost).

video(URL)

Wei-Heong Tan and Shoji Takeuchi, CIRMM/IIS, Institute of Industrial Science, University of Tokyo
* Lab on a chip, Volume 6, Issue 6, Pages 757-763, June 2006

PUBLICATIONS

  • F.tataricum
  • F.esculentum

Reconstruction pattern of the cell wall in Fagopyrum protoplast-derived hybrid cells

Sala-Cholewa, K., Milewska-Hendel, A., Pérez-Pérez, R. Grzebelus E., and Betekhtin A.

Plant Cell Tiss Organ Cult 157, 26 (2024).

  • Rice_zygotes

In Vitro Production of Zygotes by Electrofusion of Rice Gametes

Rahman MH, Toda E, Okamoto T.

Methods Mol Biol. 2020;2122:257-267.

  • Rice_zygotes

Toda E, Koiso N, Takebayashi A, Ichikawa M, Kiba T, Osakabe K, Osakabe Y, Sakakibara H, Kato N, Okamoto T

Nat Plants. 2019 Apr;5(4):363-368.

  • Giant_Liposome

A Closed System for Pico-Liter Order Substance Transport from a Giant Liposome to a Cell

Miyakawa S, Uesugi K, Morishima K.

Micromachines (Basel) . 2018 Jul 2;9(7):331.

  • Giant_Unilamellar_Vesicles

Introducing Micrometer-Sized Artificial Objects into Live Cells: A Method for Cell–Giant Unilamellar Vesicle Electrofusion

Saito AC, Ogura T, Fujiwara K, Murata S, Nomura SM.

PLoS One . 2014 Sep 17;9(9):e106853.

  • Giant_Unilamellar_Vesicles

Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules

Terasawa H, Nishimura K, Suzuki H, Matsuura T, Yomo T.

Proc Natl Acad Sci U S A . 2012 Apr 17;109(16):5942-7.

  • A.oryzae
  • A.niger

Breeding and identification of novel koji molds with high activity of acid protease by genome recombination between Aspergillus oryzae and Aspergillus niger.

Xu D, Pan L, Zhao H, Zhao M, Sun J, Liu D

J Ind Microbiol Biotechnol. 2011 Sep;38(9):1255-65.

  • Chromosomal_Analysis

A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially

Watanabe H, Suzuki H, Tateno H, Fukui Y.

J Assist Reprod Genet. 2010 Sep;27(9-10):581-8.

  • Droplets

Timing controllable electrofusion device for aqueous droplet-based microreactors

Tan WH, Takeuchi S.

Lab Chip. 2006 Jun;6(6):757-63.

  • Liposome

Utilization of Cell-Sized Lipid Containers for Nanostructure and Macromolecule Handling in Microfabricated Devices

Tresset G, Takeuchi S.

Anal Chem. 2005 May 1;77(9):2795-801.

  • E.Coli

A Microfluidic Device for Electrofusion of Biological Membranes

Tresset G, Takeuchi S.

Seisan Kenkyu, Volume 56, Number 1, Pages 101-104, 2004

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration