Other Applications using Electrical Cell Fusion

Other Applications using Electrical Cell Fusion


ECFG21 Liposome/droplet Fusion

Timing controllable electrofusion device for aqueous droplet-based microreactors

Fig. 1. Electrofusion of droplets in the fusion chamber.
(a) Droplets formed upstream enter the fusion chamber.
(b) Due to the widening of the chamber, the droplets slow down and make contact when it enters the fusion chamber.
(c) Upon the application of an electric field (50V, 750m gap, Pulse width: 10s, Interval: 0.2sec, 5 times) the contacting droplets coalesce.
(d) Photo showing two coalesced droplets. *The round particle near the top electrode was an air bubble.
Electric pulses were applied with an Electro Cell Fusion Unit (LF101, NEPA GENE).



Fig. 2. High speed camera images of the fusion process.
This fusion process is almost instantaneous. The two droplets combined into one single ”peanut-shaped” droplet within about 1ms. It took about another 5ms for the droplet to adopt a spherical shape under the effect of surface tension. Throughout the fusion process, the darker colored blue ink droplet (leftmost) was distinctly separated from the lighter colored water droplet (rightmost).


Wei-Heong Tan and Shoji Takeuchi, CIRMM/IIS, Institute of Industrial Science, University of Tokyo
* Lab on a chip, Volume 6, Issue 6, Pages 757-763, June 2006



Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration