Transfection into iPS Cells / ES Cells / Other Stem Cells by Electroporation

Longitudinal analysis of genetic and epigenetic changes in human pluripotent stem cells in the landscape of culture-induced abnormality

Kim YJ, Kang B, Kweon S, Oh S, Kim D, Gil D, Lee H, Kim JH, Ju JH, Roh TY, Hong CP, Cha HJ.

Exp Mol Med. 2024 Nov 1.

Isolation and tracing of matrix-producing notochordal and chondrocyte cells using ACAN-2A-mScarlet reporter human iPSC lines

Tong X, Poramba-Liyanage DW, van Hoolwerff M, Riemers FM, Montilla-Rojo J, Warin J, Salvatori D, Camus A, Meulenbelt I, Ramos YFM, Geijsen N, Tryfonidou MA, Shang P.

Sci Adv. 2024 Oct 25;10(43):eadp3170.

TAD boundary deletion causes PITX2-related cardiac electrical and structural defects

Baudic M, Murata H, Bosada FM, Melo US, Aizawa T, Lindenbaum P, van der Maarel LE, Guedon A, Baron E, Fremy E, Foucal A, Ishikawa T, Ushinohama H, Jurgens SJ, Choi SH, Kyndt F, Le Scouarnec S, Wakker V, Thollet A, Rajalu A, Takaki T, Ohno S, Shimizu W, Horie M, Kimura T, Ellinor PT, Petit F, Dulac Y, Bru P, Boland A, Deleuze JF, Redon R, Le Marec H, Le Tourneau T, Gourraud JB, Yoshida Y, Makita N, Vieyres C, Makiyama T, Mundlos S, Christoffels VM, Probst V, Schott JJ, Barc J.

Nat Commun. 2024 Apr 20;15(1):3380.

CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development

Hwang YS, Seita Y, Blanco MA, Sasaki K.

PLoS Genet. 2023 Dec 13;19(12):e1011080.

Temporal morphogen gradient-driven neural induction shapes single expanded neuroepithelium brain organoids with enhanced cortical identity

Anna Pagliaro, Roxy Finger, Iris Zoutendijk, Saskia Bunschuh, Hans Clevers, Delilah Hendriks, Benedetta Artegiani

Nat Commun . 2023 Nov 28;14(1):7361.

Uniform transgene activation in Tet-On systemsdepends on sustained rtTA expression

Otomo J, Woltjen K, Sakurai H.

iScience. 2023 Aug 19;26(10):107685.

Modelling renal defects in Bardet-Biedl syndrome patients using human iPS cells

James Williams, Chloe Hurling, Sabrina Munir, Peter Harley, Carolina Barcellos Machado, Ana-Maria Cujba, Mario Alvarez-Fallas, Davide Danovi, Ivo Lieberam, Rocio Sancho, Philip Beales, Fiona M Watt

Front Cell Dev Biol . 2023 Jun 2:11:1163825.

Exocyst complex component 2 is a potential host factor for SARS-CoV-2 infection

Yi R, Hashimoto R, Sakamoto A, Matsumura Y, Nagao M, Takahashi K, Takayama K.

iScience. 2022 Nov 18;25(11):105427.

Heterogeneous nuclear ribonucleoprotein U (HNRNPU) safeguards the developing mouse cortex

Sapir T, Kshirsagar A, Gorelik A, Olender T, Porat Z, Scheffer IE, Goldstein DB, Devinsky O, Reiner O.

Nat Commun. 2022 Jul 21;13(1):4209.

Panel of human cell lines with human/mouse artificial chromosomes

Uno N, Takata S, Komoto S, Miyamoto H, Nakayama Y, Osaki M, Mayuzumi R, Miyazaki N, Hando C, Abe S, Sakuma T, Yamamoto T, Suzuki T, Nakajima Y, Oshimura M, Tomizuka K, Kazuki Y.

Sci Rep. 2022 Feb 22;12(1):3009.

Generation of Monkey Induced Pluripotent Stem Cell-Derived Cartilage Lacking Major Histocompatibility Complex Class I Molecules on the Cell Surface

Okutani Y, Abe K, Yamashita A, Morioka M, Matsuda S, Tsumaki N.

Tissue Eng Part A. 2022 Jan;28(1-2):94-106.

Core-shell hydrogel microfiber-expanded pluripotent stem cell-derived lung progenitors applicable to lung reconstruction in vivo

Ikeo S, Yamamoto Y, Ikeda K, Sone N, Korogi Y, Tomiyama L, Matsumoto H, Hirai T, Hagiwara M, Gotoh S.

Biomaterials. 2021 Sep:276:121031.

The Transplantation Resistance of Type II Diabetes Mellitus Adipose-Derived Stem Cells Is Due to G6PC and IGF1 Genes Related to the FoxO Signaling Pathway

Horiguchi M, Turudome Y, Ushijima K.

Int J Mol Sci. 2021 Jun 19;22(12):6595.

A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs

Uchimura T, Asano T, Nakata T, Hotta A, Sakurai H.

Cell Rep Med. 2021 Jun 4;2(6):100298.

Toward Spatial Identities in Human Brain Organoids-on-Chip Induced by Morphogen-Soaked Beads

Lihi Ben-Reuven, Orly Reiner

Bioengineering (Basel) . 2020 Dec 18;7(4):164.

Successful Correction of ALD Patient-derived iPSCs Using CRISPR/Cas9

Eul Sik Jung, Zhejiu Quan, Mi-Yoon Chang, Wonjun Hong, Ji Hun Kim, Seung Hyun Kim, Seungkwon You, Dae-Sung Kim, Jiho Jang, Sang-Hun Lee,Hyongbum (Henry) Kim, Hoon Chul Kang

bioRxiv February 25, 2020

In vitro characterization of the human segmentation clock

Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourquié

Nature. 2020 Apr;580(7801):113-118.

Establishment of a Non-Integrate iPS Cell Line CSUASOi002-A, From Urine-Derived Cells of a Female Patient With Macular Corneal Dystrophy Carrying Compound Heterozygous CHST6 Mutations

Jing Y, Zhou Y, Wang C, Liu J, Guo Y, Mao S, Chan HF, Tang S, Chen J.

Stem Cell Res. 2019 Dec:41:101598.

Functional Evaluation of the Pathological Significance of MEFV Variants Using Induced Pluripotent Stem Cell-Derived Macrophages

Shiba T, Tanaka T, Ida H, Watanabe M, Nakaseko H, Osawa M, Shibata H, Izawa K, Yasumi T, Kawasaki Y, Saito MK, Takita J, Heike T, Nishikomori R.

J Allergy Clin Immunol. 2019 Nov;144(5):1438-1441.e12.

Persistent Epigenetic Memory Impedes Rescue of the Telomeric Phenotype in Human ICF iPSCs Following DNMT3B Correction

Toubiana S, Gagliardi M, Papa M, Manco R, Tzukerman M, Matarazzo MR, Selig S.

Elife. 2019 Nov 20:8:e47859.

Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies

Kawai K, Negoro R, Ichikawa M, Yamashita T, Deguchi S, Harada K, Hirata K, Takayama K, Mizuguchi H.

Mol Ther Methods Clin Dev. 2019 Nov 21:17:49-57

Developmental Analyses of Mouse Embryos and Adults Using a Non-Overlapping Tracing System for All Three Germ Layers

Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H.

Development. 146 (21) 2019 Nov 4

Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells

Kissling L, Monfort A, Swarts DC, Wutz A, Jinek M

Methods Enzymol. 2019;616:241-263.

Genome-wide Microhomologies Enable Precise Template-Free Editing of Biologically Relevant Deletion Mutations

Grajcarek J, Monlong J, Nishinaka-Arai Y, Nakamura M, Nagai M, Matsuo S, Lougheed D, Sakurai H, Saito MK, Bourque G, Woltjen K.

Nat Commun. 2019 Oct 24;10(1):4856.

A Versatile Toolbox for Knock-In Gene Targeting Based on the Multisite Gateway Technology

Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, Nakamura M, Sasaki E, Shiozawa S, Okano H.

PLoS One. 2019 Aug 27;14(8):e0221164.

Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells

Sato T, Higashioka K, Sakurai H, Yamamoto T, Goshima N, Ueno M, Sotozono C.

Stem Cell Reports. 2019 Aug 13;13(2):352-365.

Phenotype-Based High-Throughput Classification of Long QT Syndrome Subtypes Using Human Induced Pluripotent Stem Cells

Yoshinaga D, Baba S, Makiyama T, Shibata H, Hirata T, Akagi K, Matsuda K, Kohjitani H, Wuriyanghai Y, Umeda K, Yamamoto Y, Conklin BR, Horie M, Takita J, Heike T.

Stem Cell Reports. 2019 Aug 13;13(2):394-404.

Human iPS cells

Aberrant Axon Branching via Fos-B Dysregulation in FUS-ALS Motor Neurons

Akiyama T, Suzuki N, Ishikawa M, Fujimori K, Sone T, Kawada J, Funayama R, Fujishima F, Mitsuzawa S, Ikeda K, Ono H, Shijo T, Osana S, Shirota M, Nakagawa T, Kitajima Y, Nishiyama A, Izumi R, Morimoto S, Okada Y, Kamei T, Nishida M, Nogami M, Kaneda S, Ikeuchi Y, Mitsuhashi H, Nakayama K, Fujii T, Warita H, Okano H, Aoki M.

EBioMedicine. 2019 Jul:45:362-378.

Novel Gain-Of-Function Mutation of TRPV4 Associated With Accelerated Chondrogenic Differentiation of Dental Pulp Stem Cells Derived From a Patient With Metatropic Dysplasia

Nonaka K, Han X, Kato H, Sato H, Yamaza H, Hirofuji Y, Masuda K.

Biochem Biophys Rep. 2019 May 17:19:100648.

Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia.

Okada H, Nakanishi C, Yoshida S, Shimojima M, Yokawa J, Mori M, Tada H, Yoshimuta T, Hayashi K, Yamano T, Hanayama R, Yamagishi M, Kawashiri MA

Sci Rep. 2019 Mar 18;9(1):4695.

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Ichisima J, Suzuki NM, Samata B, Awaya T, Takahashi J, Hagiwara M, Nakahata T, Saito MK.

J Hum Genet. 2019 May;64(5):445-458.

The Lineage-Specific Transcription Factor CDX2 Navigates Dynamic Chromatin to Control Distinct Stages of Intestine Development

Kumar N, Tsai YH, Chen L, Zhou A, Banerjee KK, Saxena M, Huang S, Toke NH, Xing J, Shivdasani RA, Spence JR, Verzi MP.

Development. 2019 Mar 1;146(5):dev172189.

MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons

Nakazeki F, Tsuge I, Horie T, Imamura K, Tsukita K, Hotta A, Baba O, Kuwabara Y, Nishino T, Nakao T, Nishiga M, Nishi H, Nakashima Y, Ide Y, Koyama S, Kimura M, Tsuji S, Naitoh M, Suzuki S, Izumi Y, Kawarai T, Kaji R, Kimura T, Inoue H, Ono K

Clin Sci (Lond). 2019 Feb 22;133(4):583-595.

Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells

Iwasawa C, Tamura R, Sugiura Y, Suzuki S, Kuzumaki N, Narita M, Suematsu M, Nakamura M, Yoshida K, Toda M, Okano H, Miyoshi H.

Int J Mol Sci. 2019 Feb 14;20(4):810.

A Patient-Derived iPSC Model Revealed Oxidative Stress Increases Facioscapulohumeral Muscular Dystrophy-Causative DUX4

Sasaki-Honda M, Jonouchi T, Arai M, Hotta A, Mitsuhashi S, Nishino I, Matsuda R, Sakurai H.

Hum Mol Genet. 2018 Dec 1;27(23):4024-4035.

Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy

Minagawa A, Yoshikawa T, Yasukawa M, Hotta A, Kunitomo M, Iriguchi S, Takiguchi M, Kassai Y, Imai E, Yasui Y, Kawai Y, Zhang R, Uemura Y, Miyoshi H, Nakanishi M, Watanabe A, Hayashi A, Kawana K, Fujii T, Nakatsura T, Kaneko S.

Cell Stem Cell. 2018 Dec 6;23(6):850-858.e4.

Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes

Tanigawa S, Islam M, Sharmin S, Naganuma H, Yoshimura Y, Haque F, Era T, Nakazato H, Nakanishi K, Sakuma T, Yamamoto T, Kurihara H, Taguchi A, Nishinakamura R

Stem Cell Reports. 2018 Sep 11;11(3):727-740.

miR‑339‑5p negatively regulates loureirin A‑induced hair follicle stem cell differentiation by targeting DLX5.

Li X, Wu Y, Xie F, Zhang F, Zhang S, Zhou J, Chen D, Liu A

Mol Med Rep. 2018 Aug;18(2):1279-1286.

Human Brain Organoids on a Chip Reveal the Physics of Folding

Karzbrun E, Kshirsagar A, Cohen SR, Hanna JH, Reiner O

Nat Phys. 2018 May;14(5):515-522.

Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene.

Tanaka Y, Sone T, Higurashi N, Sakuma T, Suzuki S, Ishikawa M, Yamamoto T, Mitsui J, Tsuji H, Okano H, Hirose S

Stem Cell Res. 2018 Apr;28:100-104.

Microhomology-assisted Scarless Genome Editing in Human iPSCs

Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K.

Nat Commun. 2018 Mar 5;9(1):939.

Down-regulation of Ghrelin Receptors on Dopaminergic Neurons in the Substantia Nigra Contributes to Parkinson's Disease-Like Motor Dysfunction

Suda Y, Kuzumaki N, Sone T, Narita M, Tanaka K, Hamada Y, Iwasawa C, Shibasaki M, Maekawa A, Matsuo M, Akamatsu W, Hattori N, Okano H, Narita M.

Mol Brain, 11 (1), 6 2018 Feb 20

Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages.

Takada S, Kambe N, Kawasaki Y, Niwa A, Honda-Ozaki F, Kobayashi K, Osawa M, Nagahashi A, Semi K, Hotta A, Asaka I, Yamada Y, Nishikomori R, Heike T, Matsue H, Nakahata T, Saito MK

J Allergy Clin Immunol. 2018 Jan;141(1):339-349.e11.

Site-specific Randomization of the Endogenous Genome by a Regulatable CRISPR-Cas9 piggyBac System in Human Cells

Ishida K, Xu H, Sasakawa N, Lung MSY, Kudryashev JA, Gee P, Hotta A.

Sci Rep. 2018 Jan 10;8(1):310.

A human iPS cell myogenic differentiation system permitting high-throughput drug screening

Uchimura T, Otomo J, Sato M, Sakurai H.

Stem Cell Res. 2017 Dec:25:98-106.

CRISPR Correction of a Homozygous Low-Density Lipoprotein Receptor Mutation in Familial Hypercholesterolemia Induced Pluripotent Stem Cells

Omer L, Hudson EA, Zheng S, Hoying JB, Shan Y, Boyd NL.

Hepatol Commun. 2017 Nov;1(9):886-898.

CRISPR/Cas9-Mediated Knockin Application in Cell Therapy: A Non-viral Procedure for Bystander Treatment of Glioma in Mice.

Meca-Cortés O, Guerra-Rebollo M, Garrido C, Borrós S, Rubio N, Blanco J

Mol Ther Nucleic Acids. 2017 Sep 15:8:395-403.

Impairment of the transition from proliferative stage to prehypertrophic stage in chondrogenic differentiation of human induced pluripotent stem cells harboring the causative mutation of achondroplasia in fibroblast growth factor receptor 3

Horie N, Hikita A, Nishizawa S, Uto S, Takato T, Hoshi K.

Regen Ther. 2017 Jan 26:6:15-20.

Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9.

Higashioka K, Koizumi N, Sakurai H, Sotozono C, Sato T

Stem Cells Int. 2017;2017:9210494.

CRISPR/Cas9 Genome Editing to Repair Receptor-Mediated Endocytosis in Homozygous Familial Hypercholesterolemia Induced Pluripotent Stem Cells

Linda Omer, Elizabeth A Hudson, Jay B Hoying, Nolan L Boyd

The FASEB Journal Vol. 31, 909.2, 1 Apr 2017

Proteasome Impairment in Neural Cells Derived From HMSN-P Patient iPSCs

Murakami N, Imamura K, Izumi Y, Egawa N, Tsukita K, Enami T, Yamamoto T, Kawarai T, Kaji R, Inoue H.

Mol Brain, 10 (1), 7 2017 Feb 15

Long-term Propagation of Tree Shrew Spermatogonial Stem Cells in Culture and Successful Generation of Transgenic Offspring

Li CH, Yan LZ, Ban WZ, Tu Q, Wu Y, Wang L, Bi R, Ji S, Ma YH, Nie WH, Lv LB, Yao YG, Zhao XD, Zheng P.

Cell Res. 2017 Feb;27(2):241-252.

Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.

Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, Ueda HR, Ogawa K, Matsunaga T, Okano H

Cell Rep. 2017 Jan 3;18(1):68-81.

Genetic and Pharmacological Correction of Aberrant Dopamine Synthesis Using Patient iPSCs With BH4 Metabolism Disorders

Ishikawa T, Imamura K, Kondo T, Koshiba Y, Hara S, Ichinose H, Furujo M, Kinoshita M, Oeda T, Takahashi J, Takahashi R, Inoue H.

Hum Mol Genet. 2016 Dec 1;25(23):5188-5197.

Calcium Dysregulation Contributes to Neurodegeneration in FTLD Patient iPSC-derived Neurons

Imamura K, Sahara N, Kanaan NM, Tsukita K, Kondo T, Kutoku Y, Ohsawa Y, Sunada Y, Kawakami K, Hotta A, Yawata S, Watanabe D, Hasegawa M, Trojanowski JQ, Lee VM, Suhara T, Higuchi M, Inoue H

Sci Rep. 2016 Oct 10:6:34904.

Human Induced Pluripotent Stem Cell-Derived Podocytes Mature Into Vascularized Glomeruli Upon Experimental Transplantation

Sharmin S, Taguchi A, Kaku Y, Yoshimura Y, Ohmori T, Sakuma T, Mukoyama M, Yamamoto T, Kurihara H, Nishinakamura R.

J Am Soc Nephrol. 2016 Jun;27(6):1778-91.

Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.

Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K

Methods Mol Biol. 2016;1357:111-31.

Sall1 transiently marks undifferentiated heart precursors and regulates their fate.

Morita Y, Andersen P, Hotta A, Tsukahara Y, Sasagawa N, Hayashida N, Koga C, Nishikawa M, Saga Y, Evans SM, Koshiba-Takeuchi K, Nishinakamura R, Yoshida Y, Kwon C, Takeuchi JK

J Mol Cell Cardiol. 2016 Mar;92:158-62.

Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system.

Li HL, Gee P, Ishida K, Hotta A

Methods. 2015 Oct 23. pii: S1046-2023(15)30133-X.

Targeted Gene Correction of RUNX1 in Induced Pluripotent Stem Cells Derived From Familial Platelet Disorder With Propensity to Myeloid Malignancy Restores Normal Megakaryopoiesis

Iizuka H, Kagoya Y, Kataoka K, Yoshimi A, Miyauchi M, Taoka K, Kumano K, Yamamoto T, Hotta A, Arai S, Kurokawa M.

Exp Hematol. 2015 Oct;43(10):849-57.

Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells

Shoji E, Sakurai H, Nishino T, Nakahata T, Heike T, Awaya T, Fujii N, Manabe Y, Matsuo M, Sehara-Fujisawa A.

Sci Rep. 2015 Aug 20:5:12831.

Gene Manipulation of Human Embryonic Stem Cells by In Vitro-Synthesized mRNA for Gene Therapy

Wang XL, Yu L, Ding Y, Guo XR, Yuan YH, Li DS

Curr Gene Ther. 2015;15(4):428-35.

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9.

Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A.

Stem Cell Reports. 2015 Jan 13;4(1):143-154.