TiYO™ Autofluorescence Quenching Illuminator

Autofluorescence, a cause of background noise for microscopy, is derived from lipofuscin, elastin fibers, vitamin A, and other naturally occurring molecules, and can often make fluorescence imaging of tissues difficult.

The TiYO™ Autofluorescence Quenching Illuminator reduces autofluorescence using photo-bleaching LEDs, thereby increasing the signal-to-noise ratio.

• Insert the sample and start—no complicated procedures or special reagents required
• High power LEDs and optimized exposure complete quenching in 30 minutes to a few hours

Using TiYO, you can quench your entire sample, stain it, and then re-quench it with TiYO—allowing you to perform another round of staining!

Source: Figure 5 from Tsuneoka et al., Frontiers in Molecular Neuroscience, 2022.

Schematic illustration describing two rounds of ISH combined with IHC. First, all probes of mRNAs of interest were hybridized simultaneously. The tissue was subjected to two rounds of staining by HCR for three mRNAs, confocal imaging, and photobleaching. Then, the tissue was subjected to IHC and confocal imaging.

Unlike conventional quenching reagents, only autofluorescence signal is affected.

There is no reduction in the fluorescence from stains.

Powerful light irradiation combined with effective cooling prevents samples from overheating during photobleaching procedure.

TiYO™ was jointly developed and commercialized using the technology invented by Associate Professor Yosuke Tsuneoka, Faculty of Medicine, Toho University (patent pending: JP-A 2021-008882).

TiYO-TRAY: Tray for microscope slides

 TiYO-AD-WM: Chambers for floating or whole-mount samples (Pack of 6)