Transfection into Mouse/Rat Retina/Cornea/Spinal Cord/Sciatic Nerve by Electroporation

NEPA21

Tweezer Electrodes

Cables, Foot Switch & Others

Transfection into Mouse/Rat Retina/Cornea/Spinal Cord/Sciatic Nerve by Electroporation

APPLICATIONS

Transfection into Mouse/Rat Retina by Electroporation

Schematic illustration showing the in vivo electroporation method.
(1). DNA injection into subretinal space
(2). Electroporation

Tweezer-type electrodes (3) are placed to hold the head of newborn (P0) rat or mouse (4).

In vivo electroporated rat retinae harvested at various developmental stages.

 

(a) Whole-mount preparation of rat retina in vivo electroporated with pCAG-GFP at P0 and harvested at P21. Pictures were taken from the scleral side.

 

(b) Rat retinae in vivo electroporated with pCAG-GFP at P0 were harvested at P2(d-f ), or P5(g-i), and cryosections were prepared.

 

Takahiko Matsuda, Cepko laboratory, Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School

PUBLICATIONS

    In Vivo Sciatic Nerve

    Molecular analysis of axonal-intrinsic and glial-associated co-regulation of axon degeneration

    Catenaccio A, Llavero Hurtado M, Diaz P, Lamont DJ, Wishart TM, Court FA.

    Cell Death Dis. 2017 Nov 9;8(11):e3166

      In Vivo/Ex Vivo

      SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation

      Oriol Ros, Yvrick Zagar, Solène Ribes, Sarah Baudet, Karine Loulier, Sandrine Couvet, Delphine Ladarre, Alain Aghaie, Alice Louail, Christine Petit, Yves Mechulam, Zsolt Lenkei, Xavier Nicol

      Cell Rep, 27 (13), 4003-4012.e6 2019 Jun 25

        Ex Vivo

        Quantitative Analysis of the ThrbCRM1-centered Gene Regulatory Network

        Benjamin Souferi, Mark M Emerson

        Biol Open, 8 (4) 2019 Apr 26

          In Vivo/Ex Vivo

          Gene Transfer of Prolyl Hydroxylase Domain 2 Inhibits Hypoxia-inducible Angiogenesis in a Model of Choroidal Neovascularization.

          Takei A, Ekström M, Mammadzada P, Aronsson M, Yu M, Kvanta A, André H

          Sci Rep. 2017 Feb 10;7:42546.

            In Vivo/Ex Vivo

            Requirement of retinoic acid receptor β for genipin derivative-induced optic nerve regeneration in adult rat retina.

            Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Sugitani K, Arai K, Suzuki H, Kato S.

            PLoS One. 2013 Aug 6;8(8):e71252.

              Ex Vivo

              Identification of a Retina-Specific Otx2 Enhancer Element Active in Immature Developing Photoreceptors

              Mark M Emerson, Constance L Cepko

              Dev Biol, 360 (1), 241-55 2011 Dec 1

                Ex Vivo

                Bulk electroporation and population calcium imaging in the adult mammalian retina.

                Briggman KL, Euler T.

                J Neurophysiol. 2011 May;105(5):2601-9.

                  Ex Vivo

                  Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.

                  Ozawa Y et al.

                  J Biol Chem. 2008 Sep 5;283(36):24561-70.

                    In Vivo/Ex Vivo

                    A role for ligand-gated ion channels in rod photoreceptor development

                    Young TL, Cepko CL.

                    Neuron. 2004 Mar 25;41(6):867-79.

                      In Vivo/Ex Vivo

                      Electroporation and RNA interference in the rodent retina in vivo and in vitro

                      Matsuda T, Cepko CL.

                      Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):16-22.

                        In Vivo/Ex Vivo

                        Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach

                        Dezawa M, Takano M, Negishi H, Mo X, Oshitari T, Sawada H.

                        Micron. 2002;33(1):1-6.

                        Electroporation

                        Drug Delivery and Transfection

                        Electro Cell Fusion

                        Fluorescent Staining

                        Single-Cell/Micro-Particle Transfer

                        Cell Freezing

                        Mechanical Vibration