Transfection into Cells in Adherence by Electroporation

By using the cell-culture-plate Electrode CUY900 series with the NEPA21 electroporator, it is now possible to transfer DNA/RNA directly into cells IN ADHERENCE in a commercially available multi-well plate.

Transfection into Cells in Adherence by Electroporation


Transfection into Primary Neurons

A: 2 steps pulse electroporation using the electrodes (CUY900-13-3-5) for adherent cells.

B: EGFP fluorescence image of the neurons 2 days after electroporation.

C: High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency.

D: High magnification image of Figure C (x40). Neurites are shown clearly.

Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan.

Transfection in Cell-Culture-Plates/Dishes

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

CellsVTE CellsVTE
Primary Human Skin Fibroblasts100%50% Primary HUVEC95%75%
Primary Mouse Hippocampal Neurons
Embryonic day 14 (4 DIV)
60%50% Primary Mouse Hippocampal Neurons
Embryonic day 18 (2 DIV)
Mouse Neural Stem Cells71%50% Primary Mouse Microglial Cells
(1 DIV after 1 week co-culturing astrocyte and microglial cells)
Primary Mouse Glial Cells
(14 DIV)
80%50% Primary Mouse Stromal Cells
(1-month cultured)
Primary Mouse Liver Cells
siRNA Knock Down
Excel.89% Primary Rat Cerebral Cortex Neurons
Embryonic day 17 (2 DIV)
Primary Rat Hippocampal Neurns
Postnatal day 7 (11 DIV)
100%50% Primary Rat Granulosa CellsExcel.41%
-Human Mesenchymal Stem Cells
70%65% SH-SY5Y-Human Neuroblastoma Cells90%50%
-Human Endothelial Progenitor Cells
-Human Pancreatic Duct Epithelial Cells
-Human Acute Monocytic Leukemia Cells
90%45% C2C12-Mouse Myotubes94%60%
3T3-L1 -Mouse Embryonic Fibroblasts
(7 days after differentiation)
90%70% MEF
-Mouse Embryonic Fibroblasts
-Mouse Neuroblastoma Cells
80%90% C6
-Rat Glioma Cells

We have a lot of data of adherent-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.




Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration