Transfection into Cells in Adherence by Electroporation

By using the cell-culture-plate Electrode CUY900 series with the NEPA21 electroporator, it is now possible to transfer DNA/RNA directly into cells IN ADHERENCE in a commercially available multi-well plate.

Transfection into Cells in Adherence by Electroporation

APPLICATIONS

Transfection into Primary Neurons

A: 2 steps pulse electroporation using the electrodes (CUY900-13-3-5) for adherent cells.

B: EGFP fluorescence image of the neurons 2 days after electroporation.

C: High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency.

D: High magnification image of Figure C (x40). Neurites are shown clearly.

Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan.

Transfection in Cell-Culture-Plates/Dishes

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cells V TE   Cells V TE
Primary Human Skin Fibroblasts 100% 50%   Primary HUVEC 95% 75%
Primary Mouse Hippocampal Neurons
Embryonic day 14 (4 DIV)
60% 50%   Primary Mouse Hippocampal Neurons
Embryonic day 18 (2 DIV)
85% 54%
Mouse Neural Stem Cells 71% 50%   Primary Mouse Microglial Cells
(1 DIV after 1 week co-culturing astrocyte and microglial cells)
80% 73%
Primary Mouse Glial Cells
(14 DIV)
80% 50%   Primary Mouse Stromal Cells
(1-month cultured)
90% 50%
Primary Mouse Liver Cells
siRNA Knock Down
Excel. 89%   Primary Rat Cerebral Cortex Neurons
Embryonic day 17 (2 DIV)
70% 60%
Primary Rat Hippocampal Neurns
Postnatal day 7 (11 DIV)
100% 50%   Primary Rat Granulosa Cells Excel. 41%
hMSC
-Human Mesenchymal Stem Cells
70% 65%   SH-SY5Y-Human Neuroblastoma Cells 90% 50%
EPC
-Human Endothelial Progenitor Cells
      HPDE
-Human Pancreatic Duct Epithelial Cells
  80%
THP-1
-Human Acute Monocytic Leukemia Cells
90% 45%   C2C12-Mouse Myotubes 94% 60%
3T3-L1 -Mouse Embryonic Fibroblasts
(7 days after differentiation)
90% 70%   MEF
-Mouse Embryonic Fibroblasts
 60% 80%
Neuro-2a
-Mouse Neuroblastoma Cells
80% 90%   C6
-Rat Glioma Cells
57% 55%

We have a lot of data of adherent-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration