Transfection into Primary Cell Cultures by Electroporation

Transfection into Primary Cell Cultures by Electroporation

APPLICATIONS

Transfection Results with Nepa Gene Electroporation System

BMMC  Primary Mouse Bone Marrow-Derived Mast cells

Viability: 80%

Transfection Efficiency: 83%

FACS

bmmc_c-1

Primary Mouse Cerebral Cortex Neurons (E14)

Viability: 80%

Transfection Efficiency: 70%

Primary Human Endometrial Stromal Cells

Viability: 95%

Transfection Efficiency: 90%

Transfection Data: Primary Huyman Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
HUVEC Human Umbilical Vein Endothelial Cells95%75% HUVEC Human Umbilical Vein Endothelial Cells100%92%
HASM Human Airway Smooth Muscle Cells90%80% Human Coronary-derived Smooth Muscle Cells82%67%
Human Endometrial Stromal Cells95%90% Human Uterine Cervical Fibroblasts65%90%
Human Dermal Fibroblasts (HDF)95%89% Human Dermal Fibroblasts (HDF)100%80%
Human Keratinocytes70%65% Human Malignant Mesothelioma Cells75%55%
Human Meniscal Cells85%55% Human Colorectal Cells53%80%
Human T Cells58%90% Human T Cells50%76%
Human NK Cells48%86% PBMC Peripheral Blood Mononuclear Cells93%66%
Human Erythroblasts (CD34+ Progenitor Cells)61%34% Human Chronic Lymphocytic Leukemia (CLL)82%70%
Human Chronic Lymphocytic Leukemia (CLL) (mRNA)93%94% Human Chronic Lymphocytic Leukemia (CLL)91%83%
Human Chronic Lymphocytic Leukemia (CLL)83%93% Human Osteoblast-like Cells derived from human skull64%74%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Primary Mouse Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Mouse Cerebral Cortex Neurons (E14)80%70% Mouse Hippocampal Neurons (E14)80%60%
Mouse Hippocampal Neurons (E17)65%70% Mouse Neural Progenitor Cells80%60%
Mouse Basal Ganglia Primordium91%71% Mouse Cerebellar Granule Neurons91%65%
Mouse DRG Neurons70%70% MEF Mouse Embryonic Fibroblasts90%85%
MEF Mouse Embryonic Fibroblasts75%85% Mouse Embryonic Skin Fibroblasts80%50%
Mouse External Genital Fibroblasts (E15.5)66%59% Mouse Cervical Epithelial Cells82%55%
Mouse Vascular Adventitial Fibroblasts90%50% BMMC Mouse Bone Marrow-Derived Mast Cells80%83%
Mouse peritoneal macrophages69%41% Mouse B cells (LPS stimulated)81%73%
Mouse B cells50%61% Mouse B cells (Unstimulated)84%83%
Mouse T cells (siRNA)88%77% Naive Mouse CD8+ T cells (Cas9 RNP)50%95%
Mouse Liver Cells75%65% Mouse Osteoblast Cells85%60%
Mouse Muscle Cells68%54%    

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Primary Rat Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Rat Cerebral Cortex Neurons (E16)70%75% Rat Hippocampal Neurons60%80%
Rat Bulbar Neurons80%75% Rat Cerebellar Neurons70%55%
Rat Cerebellar Granule Cells70%80% Rat Schwann Cells90%80%
Rat Schwann Cells90%60% OEC Rat Olfactory Ensheathing Cells93%46%
Rat Müller Cells90%50% REF Rat Embryonic Fibroblasts65%65%
Rat Meningeal Fibroblasts90%95% PASMC Rat Pulmonary Artery Smooth Muscle Cells72%70%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection Data: Other Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary CellsVTE Primary CellsVTE
Chick Embryonic Fibroblasts80%90% Chick Embryonic Cerebellar Granule Cells86%83%
Bovine Cartilage Cells96%55% Goat Embryonic Epithelial Fibroblasts80%55%
Rabbit Spleen cells (B cells)70%45% Canine Adipose-Derived Stromal Cells (ADSCs) 65%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration