Transfection into Organoids by Electroporation
APPLICATIONS
Production of Genetically Modified Organoids by Electroporation
After electroporation, TP53 knockout colon organoids (TP53 KO) were obtained by selection with Nutlin-3, a niche factor for TP53.
The TP53 KO and the normal colon organoid without TP53 mutation (WT) were cultured in Nutlin-3- and Nutlin-3-free medium for 7 days, and the TP53 KO was not affected by Nutlin-3 addition because it had acquired Nutlin-3 tolerance, while the WT did not grow under Nutlin-3-supplemented conditions.
Courtesy of Professor Toshiro Sato, Dr. Mami Matano, Dr. Mariko Shimokawa, Dr. Masayuki Fujii, School of Medicine, The Sakaguchi Laboratory, Department of Organoid Medicine, Keio University
Transfection Data: Organoids
See the cell images by clicking the cell names.
V: Viability, TE: Transfection Efficiency.
Cells name | V | TE | Cells name | V | TE | |
Human Normal Fundic Gastric Organoids | 68% | Mouse Fundic Gastric Organoids | 65% | |||
Mouse Colorectal Cancer Organoids | 100% | 50% | Mouse Small Intestine Organoids |
We have a lot of data of organoids transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.
PUBLICATIONS
- Human_cervix small-cell neuroendocrine carcinoma (SCNEC)
Clonal Origin and Lineage Ambiguity in Mixed Neuroendocrine Carcinoma of the Uterine Cervix
Masamune Masuda, Keita Iida, Sadahiro Iwabuchi, Mie Tanaka, Satoshi Kubota, Hiroyuki Uematsu, Kunishige Onuma, Yoji Kukita, Kikuya Kato, Shoji Kamiura, Aya Nakajima, Roberto Coppo, Mizuki Kanda, Kiyoshi Yoshino, Yutaka Ueda, Eiichi Morii, Tadashi Kimura, Jumpei Kondo, Mariko Okada-Hatakeyama, Shinichi Hashimoto, Masahiro Inoue
Am J Pathol . 2024 Mar;194(3):415-429.
- Human_esophageal_adenocarcinoma_(EAC)
Epigenomic analyses identify FOXM1 as a key regulator of anti-tumor immune response in esophageal adenocarcinoma
Ziman B, Yang Q, Zheng Y, Sheth M, Nam C, Zhao H, Zhang L, Hu B, Bhowmick NA, Sinha UK, Lin DC.
Cell Death Dis. 2024 Feb 19;15(2):152.
- Murine_small_intestinal
Mutational inactivation of Apc in the intestinal epithelia compromises cellular organisation
Rannikmae H, Peel S, Barry S, Senda T, de la Roche M.
J Cell Sci. 2021 Jan 27;134(2):jcs250019.
- Human_large_intestinal
- Murine_small_intestinal
Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
Heinz MC, Oost KC, Snippert HJG.
STAR Protoc. 2020 Oct 8;1(3):100126.
- Cancer_tissue_originated_spheroids
Dedifferentiation of neuroendocrine carcinoma of the uterine cervix in hypoxia
Satoshi Kubota, Mie Tanaka, Hiroko Endo, Yu Ito, Kunishige Onuma, Yutaka Ueda, Shoji Kamiura, Kiyoshi Yoshino, Tadashi Kimura, Jumpei Kondo, Masahiro Inoue
Biochem Biophys Res Commun. 2020 Apr 2;524(2):398-404.
- Human_pancreatic
- Human_colorectal
- Cholangiocarcinoma(CCC)_organoids
- Human_gastric
Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
Anne-Marlen Gaebler, Alexander Hennig, Katharina Buczolich, Jürgen Weitz, Thilo Welsch, Daniel E Stange, Kristin Pape
J Vis Exp. 2020 Feb 18;(156).
Electroporation
■ Cell Cultures
- Primary Cell Cultures
- Stem Cells
- Organoids
- Cell Lines
- Cells in Adherence
■ In Vivo Mice/Rats
- Zygotes In Vitro (TAKE method)
- Zygotes In Oviduct (i-GONAD method)
- Embryos In Utero
- Ex Utero Embryos In Vitro
- Brain
- Retina / Cornea / Spinal Cord / Sciatic Nerve
- Lung / Spleen / Liver / Stomach/ Kidney / Intestine
- Pancreas / Islets of langerhans
- Testis / Ovary / Prostate / Gonad / Uterus
- Muscle / Skin / Joint / Cartilage / Tumor / Others
■ In Vivo Other Animals
- Bovine/Porcine/Other Animal Zygotes
- Hamster Zygotes in Oviduct (i-GONAD method)
- Monkey Skin
- Chicken (In Ovo・Others)
- Zebrafish & Other Fishes
- Insects・Others
■ Plant Cells & Algae
- Plant Cells
- Algae
■ Exosomes
- Exosomes
■ Bacteria, Yeast, Fungi
- E. coli and bacteria
- Yeast and Fungi
- Bacteria, Yeast, Fungi (NEPA Porator)
Drug Delivery and Transfection
■ Ultrasound Transfection and Drug Delivery (Sonoporation/Fus)
- Brain
- Liver/Skin/Other Applications
- Heart
- Cell Culture
- Lung
- Muscle
Electro Cell Fusion
■ Hybridoma Production
- Monoclonal antibodies, etc
■ Oocyte Activation
- Electrical stimulation before/after Intracytoplasmic sperm injection (ICSI)
■ Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer
- Animal cloning
■ Tetraploid Embryos Production
- 2 Cell Embryos (Tetraploid)
■ Other Applications
- Liposome・Protoplast・Yeast, etc.
Fluorescent Staining
■ Autofluorescence Quenching
- Mammalian Tissue Sections
- Fish Tissue Sections
- Amphibia tissue sections
- Avian Tissue Sections
- Plant tissue sections
Single-Cell/Micro-Particle Transfer
■ Micro targets
- Animal cells
■ Micro liquid
- Plant cells
Cell Freezing
■ Cell Therapy
- Stem cells, primary cells, etc.
■ Animal Husbandry
- Sperm, embryos, tissue, etc.