Transfection into Cell Lines by Electroporation

The Nepa Gene electroporators make it possible to achieve high transfection efficiency and high viability without resourse to special buffers. It works not only for plasmid DNA but also for siRNA, mRNA, shRNA, proteins and more.

Transfection into Cell Lines by Electroporation


Transfection into Cell Line

293T(HEK293T): Human Embryonic Kidney Cells


Transfection Effeciency:87%

Jurkat: Human T-cell Leukemia Cells


Transfection Efficiency:94%

Neuro-2a: Mouse Neuroblastoma Cells


Transfection Efficiency:90%

Transfection into Cell Lines

Species: Human

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell LinesVTE Cell LinesVTE
HeLa Human Cervical Carcinoma Cells87%93% HeLa-K Human Cervical Carcinoma Cells90%90%
293 Human Embryonic Kidney Cells92%91% 293 Human Embryonic Kidney Cells90%70%
293 Human Embryonic Kidney Cells72%85% 293T Human Embryonic Kidney Cells90%95%
293T Human Embryonic Kidney Cells83%87% 293T Human Embryonic Kidney Cells70%99%
TIG-3 Human Embryonic Lung Fibroblasts90%80% TIG-7 Human Embryonic Lung Fibroblasts89%76%
MRC-5 Human Embryonic Lung Fibroblasts85%90% WI-38 Human Embryonic Lung Fibroblasts80%70%
CCD18-Co Human Colon Fibroblasts99%61% HaCat Human Keratinocyte Cells96%69%
HDF Human Dermal Fibroblasts (106-05)90%90% TIG-109 Human Skin Fibroblasts70%60%
HFE145 Human Non-Cancerous Gastric Epithelial Cells80%50% BEAS-2B Human Bronchial Epithelial Cells75%96%
BEAS-2B Human Bronchial Epithelial Cells90%80% KMST-6 Human Fibroblasts70%60%
SUSM-1 Human Fibroblasts77%71% SW872 Human Liposarcoma Cells95%83%
HT1080 Human Fibrosarcoma Cells93%81% HT1080 Human Fibrosarcoma Cells80%90%
MG-63 Human Osteosarcoma Cells70%80% Saos-2 Human Osteosarcoma Cells80%70%
Saos-2 Human Osteosarcoma Cells60%75% U2OS Human Osteosarcoma Cells70%80%
SEKI Human Malignant Melanoma Cells 80%75% PANC-1 Human Pancreatic Carcinoma Cells78%70%
PANC-1 Human Pancreatic Carcinoma Cells55%75% MIA-PaCa-2 Human Pancreatic Carcinoma Cells80%77%
HepG2 Human Hepatoma Cells88%76% HuH-7 Human Hepatoma Cells90%86%
HuH-7 Human Hepatoma Cells82%85% HLF Human Liver Cancer Cells90%85%
H69 Human Small-Cell Lung Cancer Cells90%85%    
TFK-1 Human Bile Duct Adenocarcinoma Cells50%70% LC319 Human Lung Cancer Cells95%66%
NCI-H69 Human Small-Cell Lung Cancer Cells97%70% H1299 Human Lung Cancer Cells80%80%
H1299 Human Lung Cancer Cells90%90% HSC-2 Human Squamous Carcinoma Cells93%85%
KB31 Human Epidermoid Carcinoma Cells58%64% Ca9-22 Human Squamous Carcinoma Cells60%60%
HSC-3 Human Squamous Carcinoma Cells93%98% HEp-2 Human Laryngeal Carcinoma Cells70%90%
HGF Human Gingival Fibroblasts   MCF-7 Human Breast Cancer Cells95%80%
MCF-7 Human Breast Cancer Cells81%65% T47D Human Breast Cancer Cells90%85%
MCF-7 Human Breast Cancer Cells80%70% MDA-MB-231 Human Breast Cancer Cells85%90%
BT-20 Human Breast Cancer Cells70%80% MCF 10A Human Breast Cells  
MCF 10A Human Breast Cells90%80% A549 Human Lung Adenocarcinoma Cells85%90%
MCF 10A Human Breast Cells97%69% GC38 Human Gastric Cancer Cells80%80%
NUGC-3 Human Gastric Carcinoma Cells65%77% NUGC-3 Human Gastric Carcinoma Cells73%68%
MKN-45 Human Gastric Cancer Cells 78%73% Human Patient-Derived Gastric Cancer Cells44%74%
LNCaP Human Prostate Carcinoma71% 90% PC-3 Human Prostate Cancer Cells90%95%
DU145 Human Prostate Cancer Cells94%60% PNT2 Human Prostate Epithelial Cells85% 80%
PC-3 Human prostate Cancer Cells86%55% HCT116 Human Colon Cancer Cells 80%95%
Lovo Human Colon Adenocarcinoma Cells85% 60% HCT116 Human Colon Cancer Cells95%90%
HCT116 Human Colon Cancer Cells 80%80% Caco-2 Human Colon Cancer Cells85%80%
Caco-2 Human Colon Cancer Cells95%80% OVCAR-3 Human Colon Cancer Cells90%79%
SW620 Human Colon Cancer Cells80%80% RMG-1 Human Ovarian Clear Cell Adenocarcinoma97%67%
SKOV-3 Human Ovarian Carcinoma Cells90%90% SH-SY5Y Human Neuroblastoma Cells60%90%
SK-N-SH Human Neuroblastoma Cells95%95% SH-SY5Y Human Neuroblastoma Cells79%60%
SH-SY5Y Human Neuroblastoma Cells70%70% NB69 Human Neuroblastoma Cells95%80%
NB9 Human Neuroblastoma Cells70%70% KG-1-C Human Oligodendroglial Cells85%60%
NB-39-nu Human Neuroblastoma Cells60%63% NP3 Human Glioblastoma Cells98% 62%
A172 Human Glioblastoma Cells 85%70% 1321N1 Human Astrocytoma Cells80%80%
U87 MG Human Glioblastoma/Astrocytoma Cells70%55% Immortalized Human Pericytes83%50%
U-251 Human Glioblastoma Cells90%60% Human Dental Pulp Cells85%69%
iHAM-4 Human Amniotic Mesenchymal Cells59%95% HTR-8/Svneo Human Trophoblast Cells95%67%
Human Dental Pulp Cells90%85% RPE Retinal Pigment Epithelium Cells90%70%
SRA 01/04 Human Lens Epithelial Cells97%80% RPTEC Human Renal Proximal Tubule Epithelial Cells70%85%
RPE-1 Retinal Pigment Epithelium Cells95%80% Jurkat Human T-cell Leukemia Cells73%94%
HK-2 Human Renal Proximal Tubule Epithelial Cells50%90% Jurkat Human T-cell Leukemia Cells89%85%
Jurkat Human T-cell Leukemia Cells85%85% ED40515 Human T-cell Leukemia Cells82%84%
Jurkat Human T-cell Leukemia Cells99%92% Hut78 Human T-cell Lymphoma Cells51%74%
SNT16 Human T-cell Lymphoma Cells 85%84% Jeko-1 Human Mantle Cell Lymphoma (MCL) Cells 80%63%
Jeko-1 Human Mantle Cell Lymphoma (MCL) Cells82%71% MV4-11 Human Acute Myeloid Leukemia Cells70% 60%
MOLT-4 Human Acute Lymphoblastic Leukemia Cells95%70% MEC1 Human Chronic Lymphocytic Leukemia Cells>90%>90%
697 Human Pre-B Acute Lymphoblastic Leukemia Cells68%93% Nalm-6 Human B-cell Precursor Leukemia Cells97%76%
Nalm-6 Human B-cell Precursor Leukemia Cells77%82% KG-1 Human Acute Myeloid Leukemia Cells70% 65%
KG-1 Human Acute Myeloid Leukemia Cells60%65% PL-21 Human Acute Myeloid Leukemia Cells51%73%
MOLM-16 Human Acute Myeloid Leukemia Cells74%68% USCD/AML1 Human Leukemia Cells50%50%
Kasumi-1 Human Acute Myeloid Leukemia Cells66%79% KOPT-K1 Human T cell Acute Lymphoblastic Leukemia (T-ALL) Cells80%60%
M7 Human Acute Non Lymphocytic Leukemia85%80% GM12878 Human B-Lymphoblastoid Cells93%83%
Loucy Human T cell Acute Lymphoblastic Leukemia (T-ALL) Cells73%50% Human EBV-immortalized B Cells58%53%
T2 Human T and B lymphoblast Cells97%97% Raji Human Burkitt’s Lymphoma Cells97%83%
Namalwa Human Burkitt’s Lymphoma Cells70%75% Raji Human Burkitt’s Lymphoma Cells85%79%
Raji Human Burkitt’s Lymphoma Cells95%96% SU-DHL-4 Human Burkitt’s Lymphoma Cells79%68%
Toledo Human Burkitt’s Lymphoma Cells97%83%    
SU-DHL-10 Human B-cell Lymphoma Cells78%68% K562 Human Chronic Myelogenous Leukemia Cells91%99%
eHAP1 Human Haploid Cells72%65% K562 Human Chronic Myelogenous Leukemia Cells>90%94%
K562 Human Chronic Myelogenous Leukemia Cells>90%>90% HL-60 Human Promyelocytic Leukemia Cells80%80%
HL-60 Human Promyelocytic Leukemia Cells81%82% Mutu Ⅰ Human Burkitt Lymphoma Cells87%91%
PLB-985 Human Myeloid Leukemia Cells94%92% Ramos Human Burkitt Lymphoma Cells92%57%
Mutu III Human Burkitt Lymphoma Cells54%92% Ramos-Blue Human Burkitt Lymphoma Cells80%55%
Ramos Human Burkitt Lymphoma Cells83%57% BJAB Human EBV-negative Burkitt Lymphoma Cells96%96%
Z-138 Human Mantle Cell Lymphoma Cells93%86% TK6 Human B-Lymphoblast Cells84%79%
SKM-1 Human MDS-derived Leukaemia Cells88%83% THP-1 Human Acute Monocytic Leukemia Cells56%64%
THP-1 Human Acute Monocytic Leukemia Cells76%63% THP-1 Human Acute Monocytic Leukemia Cells 67%85%
THP-1 Human Acute Monocytic Leukemia Cells85%67% NK-92MI Human Natural Killer (NK) Cells83%95%
HMC1.2 Human Mast Leukemia Cells77%89% MTA Human Natural Killer-Like Leukemia Cells65%61%
KHYG-1 Human Natural Killer (NK)Lleukemia Cells51%71%    



Species: Mouse

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.


Cell LinesVTE Cell LinesVTE
NIH/3T3 Mouse Embryonic Fibroblasts100%90% NIH/3T3 Mouse Embryonic Fibroblasts74%81%
PT67 Mouse Fibroblasts (RetroPack PT67 cell line)91%66% 3T3-L1 Mouse Embryonic Fibroblasts (preadipocytes)90%90%
MEF Mouse Embryonic Fibroblasts90%90% MEF Mouse Embryonic Fibroblasts80%90%
STO Mouse Embryonic Fibroblasts60%51% N7 Mouse Embryonic Hypothalamic cells (immortalized)75% 100%
P19C6 Mouse Embryonic Carcinoma Cells90%50% F9 Mouse Testis Teratocarcinoma Cells85%95%
HL-1 Mouse Cardiac Muscle Cells70%70% L Mouse Fibroblasts90%65%
B16 Mouse Melanoma Cells86%76% B16 Mouse Melanoma Cells77%83%
B16 Mouse Melanoma Cells70%50% MC3T3-E1 Mouse Osteoblastic Cells85%75%
C2C12 Mouse Myoblast Cells94%90% C2C12 Mouse Myoblast Cells 90%90%
C2C12 Mouse Myoblast Cells80%70% C2C12 Mouse Myoblast Cells94%96%
bEnd.3 Mouse Brain Endothelial Cells80%80% NMuMG Mouse Mammary Gland Epithelial Cells80% 65%
Mouse Podocytes (Kidney Epithelial cells)100%84% Mouse Podocytes (Kidney Epithelial cells)66%68%
SV40 MES 13 Mouse Mesangial Cells68%72% LLc1(LL/2) Lewis Lung Cell Carcinoma 1 Cells87%81%
FM3A Mouse Breast Cancer Cells77%57% 4T1 Mouse Breast Cancer Cells90%95%
Ehrlich Mouse Ehrlich-Lettre Ascites Carcinoma Cells76%68% 3134 Mouse Mammary Adenocarcinoma Cells100%70%
Hepa1-6 Mouse Hepatoma Cells50%98% Colon-26 Mouse Colon Adenocarcinoma Cells95% 90%
S180 Mouse Sarcoma Cells72%57% LM8 Mouse Osteosarcoma Cells90%85%
ddy Mouse Endometrial Cells60% 80% MS-1 Mouse Pancreatic Endothelial Cells90%90%
AtT-20 Mouse Pituitary Tumor Cells80%80% ID-8 Mouse Ovarian Cancer Cells 95%99%
Neuro-2a Mouse Neuroblastoma Cells90%90% TtT/GF Mouse Pituitary Folliculo-Stellate-Like Cells65%83%
GL261 E9 Mouse Glioma Cells55%57% BV-2 Mouse Microglial Cells92%70%
BV-2 Mouse Microglial Cells80%80% BV-2 Mouse Microglial Cells90%50%
SIM-A9 Mouse Microglial Cells80%57%    
mDP Mouse Dental Pupilla Cells65%70% MEL Mouse Erythroleukemia Cells70%50%
L1210 Mouse Lymphocytic Leukemia Cells85%70% WR19L Mouse T-cell lymphoma Cells92%60%
EL4 Mouse T-cell lymphoma Cells87%82% BA/F3 Mouse pro-B Cells91%92%
BA/F3 Mouse pro-B Cells90%90% A20 Mouse B-cell Lymphoma Cells99% 85%
A20 Mouse B-cell Lymphoma Cells70%65% CH12F3 Mouse B Lymphoma Cells74%77%
WEHI-231 Mouse B-cell Lymphoma Cells98%73% P815 Mouse Mastocytoma Cells 67%68%
J774.1 Mouse Macrophage-like Cells100%70% RAW264.7 Mouse Macrophage-like Cells70%56%
RAW264.7 Mouse Macrophage-like Cells   RAW264.7 Mouse Macrophage-like Cells70%70%
RAW-D Mouse Macrophage-like Cells80%80% MIN6 Mouse Pancreatic Beta Cells57%71%
DC2.4 Mouse Dendritic Cells42%66% XS106 Mouse Dendritic Cells61%45%
mDC Mouse Myeloid Dendritic Cells79%72% 416B Mouse Primitive Myeloid Cells89%64%
32D Mouse Myeloid Cells 88% MLO-Y4 Mouse Osteocyte-like cells99%59%
MC/9 Mouse Mast Cells76%84% MC/9 Mouse Mast Cells87%89%
BMBa Mouse Bone marrow-derived basophils45%67% TS Mouse Trophoblast Stem Cells59%47%
Mouse hybridoma cells (lymphocytes and myeloma cells)100%66% 

Mouse T cell hybridoma cells
Courtesy of Prof. Yokosuka and Dr. Wakamatsu, Department of Immunology, Tokyo Medical University




Species: Rat

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell LinesVTE Cell LinesVTE
PC12 Rat Adrenal Pheochromocytoma Cells90%70% H9c2 Rat Ventricular Myoblasts71%82%
H9c2 Rat Ventricular Myoblasts75%80% REF Rat Embryonic Fibroblasts90%99%
RSC96 Rat Schwann Cells70%85% A7r5 Rat Aortic Smooth Muscle Cells93%75%
C6 Rat Glioma Cells80%67% UMR106 Rat Osteoblastic Cells80%70%
RCS Rat Chondrosarcoma Cells75%50% SF2 Rat Dental Epithelial Cells80%90%
HAT-7 Rat Dental Epithelial Cells80%90%    



Spiecies: Chinese Hamster

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell LinesVTE Cell LinesVTE
CHO Chinese Hamster Ovary Cells 74%90% CHO Chinese Hamster Ovary Cells98%87%
CHO Chinese Hamster Ovary Cells97%97% CHO-DG44 Chinese Hamster Ovary Cells86%80%
CHO-K1 Chinese Hamster Ovary Cells95%95% CHO-K1 Chinese Hamster Ovary Cells90%99%
CHO-S Chinese Hamster Ovary Cells94%93%    



Other Species

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Cell LinesVTE Cell LinesVTE
COS-7 African Green Monkey Kidney fibroblasts61%89% Vero African Green Monkey Kidney Epithelial Cells85%85%
MDCK Madrin-Darby Canine Kidney Cells90%95% MDCK Madrin-Darby Canine Kidney Cells 91%80%
BFF Bovine Fetal Fibroblasts93%71% BFF Bovine Fetal Fibroblasts78%72%
CKT-1 Bovine Kidney Epithelial Cells75%75% Bovine Fibroblasts90%63%
BAEC Bovine Aortic Endothelial Cells80%80% LLC-PK1 Pig Kidney Epithelial Cells80%85%
CPK Porcine Kidny Cells93%60% PGCs Chicken Primordial Germ Cells98%63%
DT40 Chichken B Cells72%85% DT40 Chichken B Cells71%60%
A6 Xenopus Kidney Epithelial Cells90%60% Exosomes (labeled DNA oligos)  

We have a lot of data of cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.




Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration