Super Electroporator NEPA21 Type III
In Vitro & In Vivo Electroporation
- Available Modes: Constant Voltage, Constant Current, and Set Current & Constant Voltage!!
- High Transfection Efficiency & Cell Viability: Achieve superior results without the need for expensive, proprietary buffers.
- Broad Cell Compatibility: Optimized for difficult-to-transfect cells, including primary cells, stem cells, immune cells, and blood cells.
- In Situ Transfection: Successfully transfect adherent cells directly in their culture plates.
- CRISPR/Cas9 Ready: An ideal platform for highly efficient genome editing.
APPLICATIONS
Electroporation
- Zygotes In Vitro (TAKE method)
- Zygotes In Oviduct (i-GONAD method)
- Embryos In Utero
- Ex Utero Embryos In Vitro
- Brain
- Retina / Cornea / Spinal Cord / Sciatic Nerve
- Lung / Spleen / Liver / Stomach/ Kidney / Intestine
- Pancreas / Islets of langerhans
- Testis / Ovary / Prostate / Gonad / Uterus
- Muscle / Skin / Joint / Cartilage / Tumor / Others
PRODUCT DESCRIPTION
NEPA21 Novel 4-Step Electroporation Pulsing Technology
NEPA21 pulse waveform（x: time y: voltage）
The novel 4-step pulsing with voltage decay results in higher transfection efficiency and higher viability WITHOUT special buffers.
- Poring Pulse Mode:
- Higher voltage, shorter duration, multiple pulses, voltage decay; This is for forming pores (small holes) in cell membrane with minimum damage.
- Polarity Exchanged Poring Pulse
- This can be applied to tissue transfection as well.
- Transfer Pulse Mode:
- Lower voltage, longer duration, multiple pulses, voltage decay; This is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum damage.
- Polarity Exchanged Transfer Pulse
- This can increase the transfection efficiency.
Comparison Table: NEPA21 Type III vs. NEPA21 Type II
| NEPA21 Type III | NEPA21 Type II | |
|---|---|---|
| Pulse Output Mode | Constant Voltage Mode Constant Current Mode Set Current & Constant Voltage Mode | Constant Voltage Mode only |
| Maximum Set Voltage | 500V | 300V |
| Maximum Output Current | 15A | 10A |
| Pre-Pulse Ohm Check | Available (Auto impedance measurement before pulsing) | Not available (Pulse output only) |
| Foot Switch | Double Foot Switch (Capable of pulse output and impedance measurement) | Single Foot Switch (Pulse output only) |
| Operation Method | Button Keys LCD Touch Panel | Button Keys only |
| Display | 7-Segment LED LCD Touch Panel | 7-Segment LED only |
| Data Output | USB Type-A (for USB flash drive) USB Type-B (for PC connection) | RS-232C (for PC connection) |
| Dedicated PC Software | Can view and delete Record Data on a PC using dedicated software. | Text output only via terminal emulator |
Constant Voltage Mode Limit Settings | Current Limit: 0.000 A to 15.00 A (Upper and lower limits settable) | Current Limit: Fixed at 2 A or 10 A (switchable) |
Constant Current Mode Limit Settings | Voltage Limit: 0.0 V to 200.0 V (Upper and lower limits settable) | N/A (Mode not available) |
Set Current & Constant Voltage Mode Limit Settings | Current Limit: 0.000 A to 15.00 A (Upper and lower limits settable) | N/A (Mode not available) |
Impedance Limit Settings | 0.000 kΩ to 99.99 kΩ (Upper and lower limits settable) | N/A |
| Program List | Up to 300 programs All set values (including limits), name, date, and time. *Includes lock setting function. | Up to 99 programs Electrical conditions only. *No lock setting function. |
Pulse Output & Resistance Measurement History | Auto-saves the latest 100 records: Date, time, all set values, all output pulse data (including total and average values), and impedance measurements. *(Data is retained even after power off) | Pulse output history: Last 1 record. Impedance measurement history: Last 1 record. *(Data is erased after power off) |
Cost-Efficiency Comparison
Is your lab constrained by transfection devices that require expensive, proprietary disposable kits?
A frequent concern we hear from the global research community is the prohibitive ongoing cost of these systems. The NEPA21 is designed to eliminate this bottleneck.
| Transfection Device | Technology & Requirements | Consumables Cost |
| NEPA21 (Nepa Gene) | Advanced electroporation without Special Buffers | Economy cuvettes: USD 2.60/reaction |
| Model N (Company L) | Electroporation requiring special buffers | Proprietary kits: USD 25.00/reaction |
| Model N (Company T) | Electroporation requiring special tips | Proprietary kits: USD 21.00/reaction |
Experience unmatched transfection efficiency with significantly lower running costs.
Proven Track Record: In vivo electroporation, zygote electroporation for genome editing, and beyond.
The NEPA21 is highly versatile, supporting not only standard cell culture electroporation but also a broad spectrum of in vivo gene transfer applications in animal models (e.g., mice and rats). This includes precise zygote electroporation for the generation of genetically modified animals.
For further details, please select your target application from the menu at the top of the page, or contact us directly.
Precise Control and Measurement of Electrical Parameters
In electroporation, strict control over fundamental electrical parameters—voltage, current, and resistance—is critical for reproducible and successful gene transfer. Ensuring the consistency of these parameters across every experiment is essential for achieving stable results.
The NEPA21 empowers researchers by accurately measuring sample resistance (impedance) and precisely delivering the required voltage and current. Furthermore, the system calculates and displays the total delivered electrical energy in Joules. This comprehensive real-time monitoring allows researchers to confidently fine-tune and achieve optimal electrical conditions for any specific application.
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