ECFG21 Super Electro Cell Fusion Generator

Hybridoma Production, Somatic-Cell Nuclear Transfer and More

  • High-performance, Downsized, and Lightweight by the latest technology
  • The world first device that can automatically read out delivered voltage and current both AC & DC pulses.
  • The fusion efficiency is 3 to 6 times higher than PEG!!
  • Besides, the new electrode chambers correspond to a large volume solution.

ECFG21 Super Electro Cell Fusion Generator

APPLICATIONS

Electro Cell Fusion

Hybridoma Production
Oocyte Activation
Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer
Tetraploid Embryos Production
Other Applications

PRODUCT DESCRIPTION

AC → 2-Step DC Pulses with Voltage Decay →  Post Fusion AC

  1. AC (Alternating Current): AC  is applied so that the lymphocyte cells (producing antibodies) & myeloma cells are aligned in a chain for touching on each side: ”Pearl Chain” Formation
  2. 2-Step DC (Direct Current) Pulses with Voltage Decay: Pores form in the aligned cells membranes are high-efficiency by 2-Step DC Pulses with Voltage Decay (Multiple pulses・Decay rate・Polarity exchanged).
  3. Post fusion (AC): The post fusion AC is applied so that the fusion process begins to mature and high fusion efficiency is achieved with low cell damage.
Electro Cell Fusion Process

The world first device that can automatically read out delivered voltage and current both AC & DC pulses.

Check the parameters not only setting,  but also automatically read out delivered voltage and current both AC & DC pulses & measurements voltage/current,
it is able to achieve the high efficient cell fusion.

ecfg21_measurements-e

 

ECFG21 Specifications

Alternate Current (AC)

Voltage 0 – 80 Vrms
Frequency 1 MHz
Duration 0 – 99 sec
Post-Fusion Duration 0 – 99 sec
Post-Fusion Decay Mode On/Off
Pause between AC / DC 5 μsec

Direct Current (DC) Pulse

Pulse Wave Square Wave
Voltage 1 – 1500 V
Pulse Length 1 – 99 μsec
Pulse Interval 0.1 – 9.9 sec
Number of Pulses 0 – 99
Decay Rate 0 – 99 %
Polarity Switching On/Off

Measurements

Voltage AC / DC
Current AC / DC
Energy (J) DC

Others

Impedance Measurement 0.01 – 50 kΩ
Operation Mode Automatic / Manual
Memory 99 programs
Dimensions / Weight 386W x 370D x 121H mm / 8.6kg

All features and specifications subject to change without notice.

RELATED PRODUCTS

Note: The specifications and appearance of products on this page are subject to change without notice.

Please feel free to contact us

Contact from here