Fluorescence Quenching of Mammalian Tissue Sections by LED Illumination
- Human: Brain, retina, tonsils, breast cancer
- Mouse: Brain, spinal cord, heart, liver, kidney, spleen, intestine, skin, oviduct, submandibular gland, adrenal gland, bone marrow, testis, embryo
- Rat: Brain, kidney
- Goat: Hypothalamus
APPLICATIONS
Mouse Brain Sections
Staining signals remain strong – Autofluorescence is eliminated
Comparative fluorescence staining data: untreated, TiYO™ pretreated, and reagent-treated samples
The results using TiYO™ (middle panels) had minimal impact on fluorescence signals other than autofluorescence, while the quenching reagent treatment resulted in a decrease in fluorescence signals.
Enhanced contrast of signals previously obscured by autofluorescence
All images were captured under identical imaging conditions and contrast settings.
PUBLICATIONS
Electroporation
■ Cell Cultures
- Primary Cell Cultures
- Stem Cells
- Organoids
- Cell Lines
- Cells in Adherence
■ In Vivo Mice/Rats
- Zygotes In Vitro (TAKE method)
- Zygotes In Oviduct (i-GONAD method)
- Embryos In Utero
- Ex Utero Embryos In Vitro
- Brain
- Retina / Cornea / Spinal Cord / Sciatic Nerve
- Lung / Spleen / Liver / Stomach/ Kidney / Intestine
- Pancreas / Islets of langerhans
- Testis / Ovary / Prostate / Gonad / Uterus
- Muscle / Skin / Joint / Cartilage / Tumor / Others
■ In Vivo Other Animals
- Bovine/Porcine/Other Animal Zygotes
- Hamster Zygotes in Oviduct (i-GONAD method)
- Monkey Skin
- Chicken (In Ovo・Others)
- Zebrafish & Other Fishes
- Insects・Others
■ Plant Cells & Algae
- Plant Cells
- Algae
■ Exosomes
- Exosomes
■ Bacteria, Yeast, Fungi
- E. coli/Bacterial Cells
- Yeasts/Fungi
- Bacterial cells/Yeasts/Fungi (NEPA Porator)
Drug Delivery and Transfection
■ Ultrasound Transfection and Drug Delivery (Sonoporation/Fus)
- Brain
- Liver/Skin/Other Applications
- Heart
- Cell Culture
- Lung
- Muscle
Electro Cell Fusion
■ Hybridoma Production
- Monoclonal antibodies, etc
■ Oocyte Activation
- Electrical stimulation before/after Intracytoplasmic sperm injection (ICSI)
■ Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer
- Animal cloning
■ Tetraploid Embryos Production
- 2 Cell Embryos (Tetraploid)
■ Other Applications
- Liposome・Protoplast・Yeast, etc.
Fluorescence Quenching / in situ Hybridization Chain Reaction
■ Autofluorescence Quenching
- Mammalian Tissue Sections
- Fish Tissue Sections
- Amphibia Tissue Sections
- Avian Tissue Sections
- Plant Tissue Sections
- Chordate Tissue Sections
■ in situ HCR
- Detection of Target mRNA
Single-Cell/Micro-Particle Transfer
■ マイクロピック＆プレースシステム
- Picking and placing micro targets
■ Micro targets
- Animal cells
■ Micro liquid
- Plant cells
Cell Freezing
■ Cell Therapy
- Stem cells, primary cells, and more
■ Animal Husbandry
- Sperm, embryos, tissues, and more