Transfection into Primary Cell Cultures by Electroporation

Transfection into Primary Cell Cultures by Electroporation

APPLICATIONS

Transfection into Primary Cells

BMMC  Primary Mouse Bone Marrow-Derived Mast cells

Viability: 80%

Transfection Efficiency: 83%

FACS

bmmc_c-1

Primary Mouse Cerebral Cortex Neurons (E14)

Viability: 80%

Transfection Efficiency: 70%

Primary Human Endometrial Stromal Cells

Viability: 95%

Transfection Efficiency: 90%

Human Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells V TE   Primary Cells V TE
HUVEC Human Umbilical Vein Endothelial Cells 95% 75%   HUVEC Human Umbilical Vein Endothelial Cells 100% 92%
HASM Human Airway Smooth Muscle Cells 90% 80%   Human Coronary-derived Smooth Muscle Cells 82% 67%
Human Endometrial Stromal Cells 95% 90%   Human Uterine Cervical Fibroblasts 65% 90%
Human Dermal Fibroblasts (HDF) 95% 89%   Human Dermal Fibroblasts (HDF) 100% 80%
Human Keratinocytes 70% 65%   Human Malignant Mesothelioma Cells 75% 55%
Human Meniscal Cells 85% 55%   Human Colorectal Cells 53% 80%
Human T Cells 58% 90%   Human T Cells 50% 76%
Human NK Cells 48% 86%   PBMC Peripheral Blood Mononuclear Cells 93% 66%
Human Erythroblasts (CD34+ Progenitor Cells) 61% 34%   Human Chronic Lymphocytic Leukemia (CLL) 82% 70%
Human Chronic Lymphocytic Leukemia (CLL) (mRNA) 93% 94%   Human Chronic Lymphocytic Leukemia (CLL) 91% 83%
Human Osteoblast-like Cells derived from human skull 64% 74%        

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Mouse Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells V TE   Primary Cells V TE
Mouse Cerebral Cortex Neurons (E14) 80% 70%   Mouse Hippocampal Neurons (E14) 80% 60%
Mouse Hippocampal Neurons (E17) 65% 70%   Mouse Neural Progenitor Cells 80% 60%
Mouse Basal Ganglia Primordium 91% 71%   Mouse Cerebellar Granule Neurons 91% 65%
Mouse DRG Neurons 70% 70%   MEF Mouse Embryonic Fibroblasts 90% 85%
MEF Mouse Embryonic Fibroblasts 75% 85%   Mouse Embryonic Skin Fibroblasts 80% 50%
Mouse External Genital Fibroblasts (E15.5) 66% 59%   Mouse Cervical Epithelial Cells 82% 55%
Mouse Vascular Adventitial Fibroblasts 90% 50%   BMMC Mouse Bone Marrow-Derived Mast Cells 80% 83%
Mouse peritoneal macrophages 69% 41%   Mouse B cells (LPS stimulated) 81% 73%
Mouse B cells 50% 61%   Mouse B cells (Unstimulated) 84% 83%
Mouse T cells (siRNA) 88% 77%   Naive Mouse CD8+ T cells (Cas9 RNP) 50% 95%
Mouse Liver Cells 75% 65%   Mouse Osteoblast Cells 85% 60%
Mouse Muscle Cells 68% 54%        

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Transfection into Rat Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells V TE   Primary Cells V TE
Rat Cerebral Cortex Neurons (E16) 70% 75%   Rat Hippocampal Neurons 60% 80%
Rat Bulbar Neurons 80% 75%   Rat Cerebellar Neurons 70% 55%
Rat Cerebellar Granule Cells 70% 80%   Rat Schwann Cells 90% 80%
Rat Schwann Cells 90% 60%   OEC Rat Olfactory Ensheathing Cells 93% 46%
Rat Müller Cells 90% 50%   REF Rat Embryonic Fibroblasts 65% 65%
Rat Meningeal Fibroblasts 90% 95%   PASMC Rat Pulmonary Artery Smooth Muscle Cells 72% 70%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

Other Primary Cells

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

Primary Cells V TE   Primary Cells V TE
Chick Embryonic Fibroblasts 80% 90%   Chick Embryonic Cerebellar Granule Cells 86% 83%
Bovine Cartilage Cells 96% 55%   Goat Embryonic Epithelial Fibroblasts 80% 55%
Rabbit Spleen cells (B cells) 70% 45%   Canine Adipose-Derived Stromal Cells (ADSCs)   65%

We have a lot of data of primary-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration