Detection of Target mRNA with ISHpalette™

ISHpalette™ in situ HCR Fluorescent Labeling

Detection of Target mRNA with ISHpalette™

APPLICATIONS

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PUBLICATIONS

  • in_situ_shHCR
  • Atlantic salmon

The fish pituitary directly responds to daylength and drives seasonality

Stephen D. McCormick, Daniel J. Hall, Marty Kwok Shing Wong, Takehiro Tsukada and Björn Thrandur Björnsson

Proc Biol Sci. 2025 Oct;292(2056):20251611.

  • in_situ_shHCR
  • Xenopus laevis

Reintegration of blastema and stump by reciprocal interaction for functional joint regeneration in frogs

Matsubara H, Inoue T, Agata K

Dev Biol., Sep:525:282-293, 2025.

  • in_situ_shHCR
  • human
  • Bright Field Detection

Aberrant Expression of Gonadotropin-Releasing Hormone Receptor in Human Mast Cells Enhances the Recruitments of Trophoblasts and NK Cells

Matsuzaki T, Hamaguchi H, Ito KR, Nakagawa R, Nakamura H, Ito H, Ueshima C, Yoshizawa A, Haga H, Kataoka TR

Am J Reprod Immunol., Sep;94(3):e70168, 2025.

  • in_situ_shHCR
  • Xenopus tropicalis

Universal Improvement of In Situ Hybridization ChainReaction by Reducing Background Signals Caused by Single Probes

Kuboe Y., Nakanishi K., Tazawa 1., Nakajima K.

Dev Growth Differ., Jul 13, 2025.

  • in_situ_shHCR
  • Arabidopsis

Strigolactones optimise plant water usage by modulating vessel formation

Zhao J, Shi D, Kaeufer K, Song C, Both D, Thier AL, Cao H, Lassen L, Xu X, Hamamura Y, Luzzietti L, Bennett T, Kaufmann K, Greb T

Nature Communications, Apr 28;16(1):3854, 2025

  • in_situ_shHCR
  • Medaka

Regulation of Branchial Anoctamin 1 Expression in Freshwater- and Seawater-Acclimated Japanese Medaka, Oryzias latipes

Konno N, Togashi A, Miyanishi H, Azuma M, Nakamachi T, Matsuda K

J Exp Zool A Ecol Integr Physiol. 2025 Apr;343(3):356-372.

  • in_situ_shHCR
  • Mouse

Type I collagen secreted in white matter lesions inhibits remyelination and functional recovery

Yamazaki R, Azuma M, Osanai Y, Kouki T, Inagaki T, Kakita A, Takao M, Ohno N

Cell Death Dis., Apr 13;16(1):285, 2025

  • in_situ_shHCR
  • Rat

Localization of Melanocortin 1 Receptor in the Substantia Nigra

Ehara A, Ito N, Nakadate K, Tokuda N.

Int. J. Mol. Sci., 26(1), 236, 2024

  • in_situ_shHCR
  • Zebrafish

Foxo3-mediated physiological cell competition ensures robust tissue patterning throughout vertebrate development

Matsumoto K, Akieda Y, Haraoka Y, Hirono N, Sasaki H, Ishitani T.

Nature Communications, Dec 17;15(1):10662, 2024

  • in_situ_shHCR
  • Mouse

Erythropoietin Production in Embryonic Neural Cells is Controlled by Hypoxia Signaling and Histone Deacetylases with an Undifferentiated Cellular State

Iwamura Y, Nakai T, Kato K, Ishioka H, Yamamoto M, Hirano I, Suzuki N.

Mol Cell Biol. Dec 2:1-14, 2024

  • in_situ_shHCR
  • Drosophila melanogaster

A fast in situ hybridization chain reaction method in Drosophila embryos and ovaries

Mikami K, Kozono Y, Masukawa M, Kobayashi S.

Fly (Austin). Dec;19(1):2428499, 2024

  • in_situ_shHCR
  • Nematostella

Neuronal and non-neuronal functions of the synaptic cell adhesion molecule neurexin in Nematostella vectensis

Guzman C, Mohri K, Nakamura R, Miyake M, Tsuchiya Y, Tomii K, Watanabe H.

Nature Communications, Aug 1;15(1):6495, 2024

  • in_situ_shHCR
  • Mouse

Primary Cilia are Required for Cell-Type Determination and Angiogenesis in Pituitary Development

Yoshida S, Tsuneoka Y, Tsukada T, Nakakura T, Kawamura A, Kai W, Yoshida K.

Endocrinology, Jul 1;165(8):bqae085, 2024

  • in_situ_shHCR
  • Mouse

Positive regulation of Hedgehog signaling via phosphorylation of GLI2/GLI3 by DYRK2 kinase

Yoshida S, Kawamura A, Aoki K, Wiriyasermkul P, Sugimoto S, Tomiyoshi J, Tajima A, Ishida Y, Katoh Y, Tsukada T, Tsuneoka Y, Yamada K, Nagamori S, Nakayama K, Yoshida K.

PNAS, Jul 9;121(28):e2320070121, 2024

  • in_situ_shHCR
  • Mouse

Reciprocal Connections between Parvalbumin-Expressing Cells and Adjacent Pyramidal Cells Are Regulated by Clustered Protocadherin γ

Kawamura N, Osuka T, Kaneko R, Kishi E, Higuchi R, Yoshimura Y, Hirabayashi T, Yagi T, Tarusawa E.

eNeuro, Oct 27;10(10):ENEURO.0250-23, 2023

  • in_situ_shHCR
  • Mouse

Characterization of TRPM8-expressing neurons in the adult mouse hypothalamus

Tsuneoka Y, Nishikawa T, Furube E, Okamoto K, Yoshimura R, Funato H, Miyata S.

Neurosci Lett., Sep 25;814:137463, 2023

  • in_situ_shHCR
  • Chum salmon

Subcellular localization of Na+/K+-ATPase isoforms resolved by in situ hybridization chain reaction in the gill of chum salmon at freshwater and seawater

Wong MKS, Tsuneoka Y, Tsukada T.

Fish Physiol Biochem., Aug;49(4):751-767, 2023

  • in_situ_shHCR
  • Mouse

Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization

Tsuneoka Y, Atsumi Y, Makanae A, Yashiro M, Funato H.

Front Mol Neurosci., Sep 2;15:976349, 2022

  • in_situ_shHCR
  • Mouse

IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies

Yamazaki S, Inohara N, Ohmuraya M, Tsuneoka Y, Yagita H, Katagiri T, Nishina T, Mikami T, Funato H, Araki K, Nakano H.

Mucosal Immunol., Jun;15(6):1321-1337, 2022

  • in_situ_shHCR
  • Eel

Gene duplication of C-type natriuretic peptide-4 (CNP4) in teleost lineage elicits subfunctionalization of ancestral CNP

Katayama Y, Saito A, Ogoshi M, Tsuneoka Y, Mukuda T, Azuma M, Kusakabe M, Takei Y, Tsukada T.

Cell Tissue Res., May;388(2):225-238, 2022

  • in_situ_shHCR
  • Mouse

GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior

Maejima et al.

PNAS, 118 (6), e2010168118, 2021

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescence Quenching / in situ Hybridization Chain Reaction

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration