Transfection into Mouse/Rat Testis, Ovary, Prostate, Gonad, and Uterus by Electroporation

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Transfection into Mouse/Rat Testis, Ovary, Prostate, Gonad, and Uterus by Electroporation

APPLICATIONS

Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

 

Stereomicroscopic views of transfected testes charged with various voltages and observed under visible (A) or excitation (B) light after 5 wk.

 

Voltage is indicated on each testis (A). 
Loss of testicular weight 5 wk after electric charge in 12-day-old testes (C). 
All values are means  SD (measured number of testes charged with 0, 10, 15, 20, 25, 30, 40, and 50 V are 4, 4, 4, 12, 12, 15, 10, and 12, respectively).

Cross-sections of a testis at 5 wk after being charged with 50 V, observed with fluorescence microscope under excitation light (D, left), followed by counterstaining of the same section with hematoxylin (D, right) or another section just stained with hematoxylin (E).

 

In higher voltage charged testes, luminal enlargement of seminiferous tubules (D) or complete degeneration of seminiferous tubules under the capsule (D, left) was observed. In these testes, many fluorescence-positive Sertoli cells were identified easily (E, right), like in 20V charged testes (Day 35).

Bars = 2mm (A, B) or 100μm (D, E).

 

Kentaro Yomogida, School of Human Environmental Sciences, Mukogawa Women’s University
*BIOLOGY of REPRODUCTION, Volume 67, Issue 3, Pages 712-717, September 2002

PUBLICATIONS

  • Testis

Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes

Dereli I, Telychko V, Papanikos F, Raveendran K, Xu J, Boekhout M, Stanzione M, Neuditschko B, Imjeti NS, Selezneva E, Tuncay H, Demir S, Giannattasio T, Gentzel M, Bondarieva A, Stevense M, Barchi M, Schnittger A, Weir JR, Herzog F, Keeney S, Tóth A.

Nat Commun. 2024 Apr 5;15(1):2941.

  • Endometrium

Multiplexed genome editing by in vivo electroporation of Cas9 ribonucleoproteins effectively induces endometrial carcinoma in mice

Kobayashi R, Kawabata-Iwakawa R, Sugiyama M, Oyama T, Ohtsuka M, Horii T, Morita S, Nishiyama M, Hatada I.

Int J Cancer. 2023 Jun 1;152(11):2331-2337.

  • Ovary

Senescence induction dictates response to chemo- and immunotherapy in preclinical models of ovarian cancer

Paffenholz SV, Salvagno C, Ho YJ, Limjoco M, Baslan T, Tian S, Kulick A, de Stanchina E, Wilkinson JE, Barriga FM, Zamarin D, Cubillos-Ruiz JR, Leibold J, Lowe SW.

Proc Natl Acad Sci U S A. 2022 Feb 1;119(5):e2117754119.

  • Gonads
  • Ex_Vivo

Divergent Roles of CYP26B1 and Endogenous Retinoic Acid in Mouse Fetal Gonads

Bellutti L, Abby E, Tourpin S, Messiaen S, Moison D, Trautmann E, Guerquin MJ, Rouiller-Fabre V, Habert R, Livera G.

Biomolecules . 2019 Sep 26;9(10):536.

  • Prostate

Somatic Tissue Engineering in Mouse Models Reveals an Actionable Role for WNT Pathway Alterations in Prostate Cancer Metastasis

Leibold J, Ruscetti M, Cao Z, Ho YJ, Baslan T, Zou M, Abida W, Feucht J, Han T, Barriga FM, Tsanov KM, Zamechek L, Kulick A, Amor C, Tian S, Rybczyk K, Salgado NR, Sánchez-Rivera FJ, Watson PA, de Stanchina E, Wilkinson JE, Dow LE, Abate-Shen C, Sawyers CL, Lowe SW.

Cancer Discov. 2020 Jul;10(7):1038-1057.

  • Endometrium

A novel method of gene transduction to the murine endometrium using in vivo electroporation.

Kobayashi R, Endo K, Ohmori Y, Hondo E.

J Vet Med Sci . 2017 Sep 29;79(9):1573-1577.

  • Testis

The Enigmatic Meiotic Dense Body and Its Newly Discovered Component, SCML1, Are Dispensable for Fertility and Gametogenesis in Mice

Frantzeskos Papanikos, Katrin Daniel, Angelique Goercharn-Ramlal, Ji-Feng Fei, Thomas Kurth, Lukasz Wojtasz, Ihsan Dereli, Jun Fu, Josef Penninger, Bianca Habermann, Azim Surani, A Francis Stewart, Attila Toth

Chromosoma, 126 (3), 399-415 Jun 2017

  • Testis

In Vivo Microinjection and Electroporation of Mouse Testis.

Michaelis M, Sobczak A, Weitzel JM.

J Vis Exp. 2014 Aug 23;(90).

  • Testis

cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis

Somboonthum P, Ohta H, Yamada S, Onishi M, Ike A, Nishimune Y, Nozaki M.

Nucleic Acids Res. 2005 Jun 10;33(10):3401-11.

  • Testis

Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation

Ike A, Ohta H, Onishi M, Iguchi N, Nishimune Y, Nozaki M.

FEBS Lett. 2004 Feb 13;559(1-3):159-64.

  • Testis

Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells

Hisano M, Ohta H, Nishimune Y, Nozaki M.

Nucleic Acids Res. 2003 Aug 15;31(16):4797-804.

  • Testis

Dramatic Expansion of Germinal Stem Cells by Ectopically Expressed Human Glial Cell Line-Derived Neurotrophic Factor in Mouse Sertoli Cells

Yomogida K, Yagura Y, Tadokoro Y, Nishimune Y.

Biol Reprod. 2003 Oct;69(4):1303-7.

  • Ovary

Effect of CaCl2 concentration on the rate of foreign gene transfer and expression by in vivo electroporation in the mouse ovary

Suzuki T, Tsunekawa J, Murai A, Muramatsu T.

Int J Mol Med. 2003 Sep;12(3):365-8.

  • Testis

Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

Yomogida K, Yagura Y, Nishimune Y.

Biol Reprod. 2002 Sep;67(3):712-7.

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration