Transfection into Cells in Adherence by Electroporation

NEPA21

Cell-Culture-Plate Electrodes

Cables, Foot Switch & Others

By using the cell-culture-plate Electrode CUY900 series with the NEPA21 electroporator, it is now possible to transfer DNA/RNA directly into cells IN ADHERENCE in a commercially available multi-well plate.

Transfection into Cells in Adherence by Electroporation

APPLICATIONS

Transfection into Primary Neurons

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A: 2 steps pulse electroporation using the electrodes (CUY900-13-3-5) for adherent cells.

B: EGFP fluorescence image of the neurons 2 days after electroporation.

C: High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency.

D: High magnification image of Figure C (x40). Neurites are shown clearly.

Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan.

Transfection in Cell-Culture-Plates/Dishes

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

CellsVTE CellsVTE
Primary Human Skin Fibroblasts100%50% Primary HUVEC95%75%
Primary Mouse Hippocampal Neurons
Embryonic day 14 (4 DIV)		60%50% Primary Mouse Hippocampal Neurons
Embryonic day 18 (2 DIV)		85%54%
Mouse Neural Stem Cells71%50% Primary Mouse Microglial Cells
(1 DIV after 1 week co-culturing astrocyte and microglial cells)		80%73%
Primary Mouse Glial Cells
(14 DIV)		80%50% Primary Mouse Stromal Cells
(1-month cultured)		90%50%
Primary Mouse Liver Cells
siRNA Knock Down		Excel.89% Primary Rat Cerebral Cortex Neurons
Embryonic day 17 (2 DIV)		70％60%
Primary Rat Hippocampal Neurns
Postnatal day 7 (11 DIV)		100%50% Primary Rat Granulosa CellsExcel.41％
hMSC
-Human Mesenchymal Stem Cells		70%65% SH-SY5Y-Human Neuroblastoma Cells90%50%
EPC
-Human Endothelial Progenitor Cells		   HPDE
-Human Pancreatic Duct Epithelial Cells		 80%
THP-1
-Human Acute Monocytic Leukemia Cells		90%45% C2C12-Mouse Myotubes94%60%
3T3-L1 -Mouse Embryonic Fibroblasts
(7 days after differentiation)		90%70% MEF
-Mouse Embryonic Fibroblasts		 60%80%
Neuro-2a
-Mouse Neuroblastoma Cells		80%90% C6
-Rat Glioma Cells		57%55%

We have a lot of data of adherent-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

    HEK293T/HeLa

    Targeted protein degradation by Trim-Away using cell resealing coupled with microscopic image-based quantitative analysis

    Kunishige R, Murata M, Kano F.

    Front Cell Dev Biol. 2022 Dec 19:10:1027043.

      Mouce Müller Glia

      Apolipoprotein E-Containing Lipoproteins and LRP1 Protect From NMDA-Induced Excitotoxicity Associated With Reducing α2-Macroglobulin in Müller Glia

      Hayashi H, Mori M, Harashima M, Hashizume T, Furiya M, Mukaigaito C, Takemura E, Yamada M, Mise K, Yuan B, Takagi N.

      Invest Ophthalmol Vis Sci. 2021 Oct 4;62(13):23.

        Primary mouse/rat cortical neurons

        Neuronal Ca 2+ -dependent activator protein 1 (NCDAP1) induces neuronal cell death by activating p53 pathway following traumatic brain injury

        Masaaki Arai, Osamu Imamura, Nobuo Kondoh, Minori Dateki, Kunio Takishima

        J Neurochem. 2019 Dec;151(6):795-809.

          C2C12 myotubes

          mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4

          Castets P, Rion N, Théodore M, Falcetta D, Lin S, Reischl M, Wild F, Guérard L, Eickhorst C, Brockhoff M, Guridi M, Ibebunjo C, Cruz J, Sinnreich M, Rudolf R, Glass DJ, Rüegg MA.

          Nat Commun. 2019 Jul 18;10(1):3187.

            Mice hippocampal primary neurons (DIV 6)

            The Interactome of Palmitoyl-Protein Thioesterase 1 (PPT1) Affects Neuronal Morphology and Function

            Sapir T, Segal M, Grigoryan G, Hansson KM, James P, Segal M, Reiner O.

            Front Cell Neurosci. 2019 Mar 13:13:92.

              C2C12 myotubes

              UBE2E1 Is Preferentially Expressed in the Cytoplasm of Slow-Twitch Fibers and Protects Skeletal Muscles from Exacerbated Atrophy upon Dexamethasone Treatment.

              Cécile P, Julien A, Andrea A, Agnès C, Cécile CG, Clara T, Christiane D, Lydie C, Daniel B, Marco S, Didier A, Daniel T

              Cells. 2018 Nov 16;7(11). pii: E214.

                Primary human epidermal keratinocytes (HPEKs)

                Characterization of centriole duplication in human epidermis, Bowen's disease, and squamous cell carcinoma.

                Watanuki S, Fujita H, Kouyama K, Amagai M, Kubo A

                J Dermatol Sci. 2018 Jul;91(1):9-18.

                  Primary rat DRG neurons

                  MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.

                  Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, Hoogenraad CC

                  Neuron. 2017 Apr 19;94(2):347-362.e7.

                    COS-7

                    Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots.

                    Katrukha EA, Mikhaylova M, van Brakel HX, van Bergen En Henegouwen PM, Akhmanova A, Hoogenraad CC1, Kapitein LC

                    Nat Commun. 2017 Mar 21;8:14772.

                      C2C12 myotubes

                      Palmitate increases musclin gene expression through activation of PERK signaling pathway in C2C12 myotubes.

                      Gu N, Guo Q, Mao K, Hu H, Jin S, Zhou Y, He H, Oh Y, Liu C, Wu Q

                      Biochem Biophys Res Commun. 2015 Nov 20;467(3):521-6.

                        Primary mouse myoblasts

                        Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration.

                        Umansky KB, Gruenbaum-Cohen Y, Tsoory M, Feldmesser E, Goldenberg D, Brenner O, Groner Y

                        PLoS Genet. 2015 Aug 14;11(8):e1005457.

                          Retinal Ganglion Cells from human iPSCs

                          Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells.

                          Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N

                          Sci Rep. 2015 Feb 10;5:8344.

                            C2C12 myotubes

                            LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner.

                            Ohkawara B, Cabrera-Serrano M, Nakata T, Milone M, Asai N, Ito K, Ito M, Masuda A, Ito Y, Engel AG, Ohno K

                            Hum Mol Genet. 2013 Nov 29. [Epub ahead of print]

                              Primary mouse hippocampus and cortex

                              Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons.

                              Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O.

                              J Neurosci. 2013 Jul 17;33(29):11932-48.

                              Electroporation

                              Drug Delivery and Transfection

                              Electro Cell Fusion

                              Fluorescent Staining

                              Single-Cell/Micro-Particle Transfer

                              Cell Freezing

                              Mechanical Vibration