Transfection into Cells in Adherence by Electroporation
By using the cell-culture-plate Electrode CUY900 series with the NEPA21 electroporator, it is now possible to transfer DNA/RNA directly into cells IN ADHERENCE in a commercially available multi-well plate.
APPLICATIONS
Transfection into Primary Neurons
A: 2 steps pulse electroporation using the electrodes (CUY900-13-3-5) for adherent cells.
B: EGFP fluorescence image of the neurons 2 days after electroporation.
C: High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency.
D: High magnification image of Figure C (x40). Neurites are shown clearly.
Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan.
Transfection in Cell-Culture-Plates/Dishes
See the cell images by clicking the cell names.
V: Viability, TE: Transfection Efficiency.
Cells | V | TE | Cells | V | TE | |
Primary Human Skin Fibroblasts | 100% | 50% | Primary HUVEC | 95% | 75% | |
Primary Mouse Hippocampal Neurons Embryonic day 14 (4 DIV) | 60% | 50% | Primary Mouse Hippocampal Neurons Embryonic day 18 (2 DIV) | 85% | 54% | |
Mouse Neural Stem Cells | 71% | 50% | Primary Mouse Microglial Cells (1 DIV after 1 week co-culturing astrocyte and microglial cells) | 80% | 73% | |
Primary Mouse Glial Cells (14 DIV) | 80% | 50% | Primary Mouse Stromal Cells (1-month cultured) | 90% | 50% | |
Primary Mouse Liver Cells siRNA Knock Down | Excel. | 89% | Primary Rat Cerebral Cortex Neurons Embryonic day 17 (2 DIV) | 70% | 60% | |
Primary Rat Hippocampal Neurns Postnatal day 7 (11 DIV) | 100% | 50% | Primary Rat Granulosa Cells | Excel. | 41% | |
hMSC -Human Mesenchymal Stem Cells | 70% | 65% | SH-SY5Y-Human Neuroblastoma Cells | 90% | 50% | |
EPC -Human Endothelial Progenitor Cells | HPDE -Human Pancreatic Duct Epithelial Cells | 80% | ||||
THP-1 -Human Acute Monocytic Leukemia Cells | 90% | 45% | C2C12-Mouse Myotubes | 94% | 60% | |
3T3-L1 -Mouse Embryonic Fibroblasts (7 days after differentiation) | 90% | 70% | MEF -Mouse Embryonic Fibroblasts | 60% | 80% | |
Neuro-2a -Mouse Neuroblastoma Cells | 80% | 90% | C6 -Rat Glioma Cells | 57% | 55% |
We have a lot of data of adherent-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.
PUBLICATIONS
Electroporation
■ Cell Cultures
- Primary Cell Cultures
- Stem Cells
- Organoids
- Cell Lines
- Cells in Adherence
■ In Vivo Mice/Rats
- Zygotes In Vitro (TAKE method)
- Zygotes In Oviduct (i-GONAD method)
- Embryos In Utero
- Ex Utero Embryos In Vitro
- Brain
- Retina / Cornea / Spinal Cord / Sciatic Nerve
- Lung / Spleen / Liver / Stomach/ Kidney / Intestine
- Pancreas / Islets of langerhans
- Testis / Ovary / Prostate / Gonad / Uterus
- Muscle / Skin / Joint / Cartilage / Tumor / Others
■ In Vivo Other Animals
- Bovine/Porcine/Other Animal Zygotes
- Hamster Zygotes in Oviduct (i-GONAD method)
- Monkey Skin
- Chicken (In Ovo・Others)
- Zebrafish & Other Fishes
- Insects・Others
■ Plant Cells & Algae
- Plant Cells
- Algae
■ Exosomes
- Exosomes
■ Bacteria, Yeast, Fungi
- E. coli and bacteria
- Yeast and Fungi
- Bacteria, Yeast, Fungi (NEPA Porator)
Drug Delivery and Transfection
■ Ultrasound Transfection and Drug Delivery (Sonoporation/Fus)
- Brain
- Liver/Skin/Other Applications
- Heart
- Cell Culture
- Lung
- Muscle
Electro Cell Fusion
■ Hybridoma Production
- Monoclonal antibodies, etc
■ Oocyte Activation
- Electrical stimulation before/after Intracytoplasmic sperm injection (ICSI)
■ Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer
- Animal cloning
■ Tetraploid Embryos Production
- 2 Cell Embryos (Tetraploid)
■ Other Applications
- Liposome・Protoplast・Yeast, etc.
Fluorescent Staining
■ Autofluorescence Quenching
- Mammalian Tissue Sections
- Fish Tissue Sections
- Amphibia tissue sections
- Avian Tissue Sections
- Plant tissue sections
Single-Cell/Micro-Particle Transfer
■ Micro targets
- Animal cells
■ Micro liquid
- Plant cells
Cell Freezing
■ Cell Therapy
- Stem cells, primary cells, etc.
■ Animal Husbandry
- Sperm, embryos, tissue, etc.