Transfection into Cells in Adherence by Electroporation

NEPA21

Cell-Culture-Plate Electrodes

Cables, Foot Switch & Others

By using the cell-culture-plate Electrode CUY900 series with the NEPA21 electroporator, it is now possible to transfer DNA/RNA directly into cells IN ADHERENCE in a commercially available multi-well plate.

Transfection into Cells in Adherence by Electroporation

APPLICATIONS

Transfection into Primary Neurons

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A: 2 steps pulse electroporation using the electrodes (CUY900-13-3-5) for adherent cells.

B: EGFP fluorescence image of the neurons 2 days after electroporation.

C: High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency.

D: High magnification image of Figure C (x40). Neurites are shown clearly.

Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan.

Transfection in Cell-Culture-Plates/Dishes

See the cell images by clicking the cell names.

V: Viability, TE: Transfection Efficiency.

CellsVTE CellsVTE
Primary Human Skin Fibroblasts100%50% Primary HUVEC95%75%
Primary Mouse Hippocampal Neurons
Embryonic day 14 (4 DIV)		60%50% Primary Mouse Hippocampal Neurons
Embryonic day 18 (2 DIV)		85%54%
Mouse Neural Stem Cells71%50% Primary Mouse Microglial Cells
(1 DIV after 1 week co-culturing astrocyte and microglial cells)		80%73%
Primary Mouse Glial Cells
(14 DIV)		80%50% Primary Mouse Stromal Cells
(1-month cultured)		90%50%
Primary Mouse Liver Cells
siRNA Knock Down		Excel.89% Primary Rat Cerebral Cortex Neurons
Embryonic day 17 (2 DIV)		70％60%
Primary Rat Hippocampal Neurns
Postnatal day 7 (11 DIV)		100%50% Primary Rat Granulosa CellsExcel.41％
hMSC
-Human Mesenchymal Stem Cells		70%65% SH-SY5Y-Human Neuroblastoma Cells90%50%
EPC
-Human Endothelial Progenitor Cells		   HPDE
-Human Pancreatic Duct Epithelial Cells		 80%
THP-1
-Human Acute Monocytic Leukemia Cells		90%45% C2C12-Mouse Myotubes94%60%
3T3-L1 -Mouse Embryonic Fibroblasts
(7 days after differentiation)		90%70% MEF
-Mouse Embryonic Fibroblasts		 60%80%
Neuro-2a
-Mouse Neuroblastoma Cells		80%90% C6
-Rat Glioma Cells		57%55%

We have a lot of data of adherent-cell transfection with high efficiency and high viability. Please feel to free to contact us for the latest data.

 

PUBLICATIONS

    Primary mouse/rat cortical neurons

    Neuronal Ca 2+ -dependent activator protein 1 (NCDAP1) induces neuronal cell death by activating p53 pathway following traumatic brain injury

    Masaaki Arai, Osamu Imamura, Nobuo Kondoh, Minori Dateki, Kunio Takishima

    J Neurochem. 2019 Dec;151(6):795-809.

      C2C12 myotubes

      UBE2E1 Is Preferentially Expressed in the Cytoplasm of Slow-Twitch Fibers and Protects Skeletal Muscles from Exacerbated Atrophy upon Dexamethasone Treatment.

      Cécile P, Julien A, Andrea A, Agnès C, Cécile CG, Clara T, Christiane D, Lydie C, Daniel B, Marco S, Didier A, Daniel T

      Cells. 2018 Nov 16;7(11). pii: E214.

        Primary human epidermal keratinocytes

        Characterization of centriole duplication in human epidermis, Bowen's disease, and squamous cell carcinoma.

        Watanuki S, Fujita H, Kouyama K, Amagai M, Kubo A

        J Dermatol Sci. 2018 Jul;91(1):9-18.

          Primary rat DRG neurons

          MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.

          Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, Hoogenraad CC

          Neuron. 2017 Apr 19;94(2):347-362.e7.

            COS-7

            Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots.

            Katrukha EA, Mikhaylova M, van Brakel HX, van Bergen En Henegouwen PM, Akhmanova A, Hoogenraad CC1, Kapitein LC

            Nat Commun. 2017 Mar 21;8:14772.

              C2C12 myotubes

              Palmitate increases musclin gene expression through activation of PERK signaling pathway in C2C12 myotubes.

              Gu N, Guo Q, Mao K, Hu H, Jin S, Zhou Y, He H, Oh Y, Liu C, Wu Q

              Biochem Biophys Res Commun. 2015 Nov 20;467(3):521-6.

                Primary mouse myoblasts

                Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration.

                Umansky KB, Gruenbaum-Cohen Y, Tsoory M, Feldmesser E, Goldenberg D, Brenner O, Groner Y

                PLoS Genet. 2015 Aug 14;11(8):e1005457.

                  Retinal Ganglion Cells from human iPSCs

                  Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells.

                  Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N

                  Sci Rep. 2015 Feb 10;5:8344.

                    C2C12 myotubes

                    LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner.

                    Ohkawara B, Cabrera-Serrano M, Nakata T, Milone M, Asai N, Ito K, Ito M, Masuda A, Ito Y, Engel AG, Ohno K

                    Hum Mol Genet. 2013 Nov 29. [Epub ahead of print]

                      Primary mouse hippocampus and cortex

                      Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons.

                      Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O.

                      J Neurosci. 2013 Jul 17;33(29):11932-48.

                      Electroporation

                      Drug Delivery and Transfection

                      Electro Cell Fusion

                      Fluorescent Staining

                      Single-Cell/Micro-Particle Transfer

                      Cell Freezing

                      Mechanical Vibration