Freezing of stem cells, primary cells, cord blood, etc.
Accurate temperature control allows for high cell viability.
APPLICATIONS
Viability after freezing
Viability rates after freezing human dental pulp stem cells (DPSCs), human periodontal ligament stem cells (PDLFs), human bone marrow stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs)
Each cell was suspended according to the protocol of the cryoprotectant, filled into 2-mL cryo tubes, and cooled to -80°C at -1°C/min in PF-NP-200, resulting in high viability rates.
Mouse proB lymphocyte cells (Ba/F3)
Ba/F3 cells were suspended according to the protocol of the freezing agent, filled into freeze bags, and cooled to -80°C in a PF-NP freezer at -1°C/min, resulting in a high survival rate comparable to that of 2-mL cryovials.
*Collaboration with Excellent Young Researcher (LEADER) Shinya Yamahira, St. Luke’s International Univrsity, Center for Medical Sciences
Electroporation
■ Cell Cultures
- Primary Cell Cultures
- Stem Cells
- Organoids
- Cell Lines
- Cells in Adherence
■ In Vivo Mice/Rats
- Zygotes In Vitro (TAKE method)
- Zygotes In Oviduct (i-GONAD method)
- Embryos In Utero
- Embryos In Vitro
- Brain
- Retina / Cornea / Spinal Cord / Sciatic Nerve
- Lung / Spleen / Liver / Stomach/ Kidney / Intestine
- Pancreas / Islets of langerhans
- Testis / Ovary / Prostate / Gonad / Uterus
- Muscle / Skin / Joint / Cartilage / Tumor / Others
■ In Vivo Other Animals
- Bovine/Porcine/Other Animal Zygotes
- Hamster Zygotes in Oviduct (i-GONAD method)
- Monkey Skin
- Chicken (In Ovo・Others)
- Zebrafish & Other Fishes
- Insects・Others
■ Plant Cells & Algae
- Plant Cells
- Algae
■ Exosomes
- Exosomes
■ Bacteria, Yeast, Fungi
- E. coli and bacteria
- Yeast and fungi
- Bacteria, Yeast, Fungi (NEPA Porator)
Drug Delivery and Transfection
■ Ultrasound Transfection and Drug Delivery (Sonoporation/Fus)
- Brain
- Liver/Skin/Other Applications
- Heart
- Cell Culture
- Lung
- Muscle
Electro Cell Fusion
■ Hybridoma Production
- Monoclonal antibodies, etc
■ Oocyte Activation
- Electrical stimulation before/after Intracytoplasmic sperm injection (ICSI)
■ Somatic cell nuclear transfer (SCNT)・ Oocytes Nuclear Transfer
- Animal cloning
■ Tetraploid Embryos Production
- 2 Cell Embryos (Tetraploid)
■ Other Applications
- Liposome・Protoplast・Yeast, etc.
Fluorescent Staining
■ Autofluorescence Quenching
- Mammalian Tissue Sections
- Fish Tissue Sections
- Amphibia tissue sections
- Avian Tissue Sections
- Plant tissue sections
Single-Cell/Micro-Particle Transfer
■ Micro targets
- Animal cells
■ Micro liquid
- Plant cells
Cell Freezing
■ Cell Therapy
- Stem cells, Primary cells, and etc.
■ Animal Husbandry
- Sperm, embryos, tissue, etc.