Freezing of stem cells, primary cells, cord blood, etc.

PF-NP-200

Accurate temperature control allows for high cell viability.

Freezing of stem cells, primary cells, cord blood, etc.

APPLICATIONS

Viability after freezing

Viability rates after freezing human dental pulp stem cells (DPSCs), human periodontal ligament stem cells (PDLFs), human bone marrow stem cells (BMSCs), human umbilical vein endothelial cells (HUVECs)

Each cell was suspended according to the protocol of the cryoprotectant, filled into 2-mL cryovials, and cooled to -80°C at -1°C/min in PF-NP-200, resulting in high viability rates.

Freezer viability cryovial

Mouse proB lymphocyte cells (Ba/F3)

Ba/F3 cells were suspended according to the protocol of the freezing agent, filled into cell culture bags, and cooled to -80°C in a PF-NP freezer at -1°C/min, resulting in a high survival rate comparable to that of 2-mL cryovials.

Freezer viability cell culture bag

 

*Collaboration with Excellent Young Researcher (LEADER) Shinya Yamahira, St. Luke’s International University, Center for Medical Sciences

PUBLICATIONS

  • Human iPS cells

Detection of residual pluripotent stem cells in cell therapy products utilizing droplet digital PCR: an international multisite evaluation study

Satoshi Yasuda, Kiyoko Bando, Marianne P Henry, Silvana Libertini, Takeshi Watanabe, Hiroto Bando, Connie Chen, Koki Fujimori, Kosuke Harada, Takuya Kuroda, Myriam Lemmens, Dragos Marginean, David Moss, Lucilia Pereira Mouriès, Nicole S Nicholas, Matthew J K Smart, Orie Terai, Yoji Sato

Stem Cells Transl Med . 2024 Aug 9:szae058.

Electroporation

Drug Delivery and Transfection

Electro Cell Fusion

Fluorescent Staining

Single-Cell/Micro-Particle Transfer

Cell Freezing

Mechanical Vibration