エレクトロポレーションアプリケーション別文献

NEPA21遺伝子導入装置が用いられた文献（下位機種CUY21遺伝子導入装置も含む）

Cell Cultures (suspension electroporation)

	Human iPS cells		Reprogramming of iPS cells
	Human ES cells
	Human dissociated organoids		Human organoids
	Cancer tissue-originated spheroids
	Human adipose mesenchymal stem cells (hAMSCs)		Human dental pulp stem cells (DPSCs)
	Primary human skin fibroblasts		Primary human hepatocytes
	PBMC		Primary human T cells
	Primary human NK cells		Primary human B cells
	Primary human airway epithelial basal cells
	HEK293 human embryonic kidney cells		TIG-7 human embryonic lung fibroblasts and HeLa human cervical carcinoma cell line
	U251 human glioma cell line		HCS-2/8 human chondrocytic cell line
	Jurkat human T-cell Leukemia cells		ME-1 human AML cells and A-E/CD34+ human preleukemic cells
	CTL Cytotoxic T Lympocytes		HeLa human cervical carcinoma cells
	PC-3 human prostate cancer cells		PLB-985 human myeloid leukemia cells

	Mouse ES cells		Mouse dissociated organoids
	Primary mouse neurons		Primary mouse astrocytes
	Primary mouse bone marrow-derived mast cells (BMMCs)		Primary mouse hematopoietic stem cells (HSCs)
	Ba/F3 mouse pro-B cell line		C2C12 mouse myoblast cell line
	MEF mouse embryonic fibroblasts and 3T3-L1 mouse embryonic fibroblasts		3T3-L1 mouse preadipocyte
	NIH/3T3 mouse embryonic fibroblasts		RAW264.7 mouse macrophage-like cell line
	ZS mouse sms-deficient immortalized embryonic fibroblasts		Hepa1-6 mouse hepatoma cells

	Primary rat neurons		RSC96 rat Schwann cell line
	Rat hair follicle stem cells		Rat dermal fibroblast sheets

	Shrew spermatogonial stem cells (SSCs)		CHO chinese hamser ovary cells
	Primary chick spinal cord neurons		MDCK/MDCK II madrin-darby canine kidney cell line
	Primary cynomolgus PBMCs		Primary rhesus macaque CD4+ T cells
	COS-7 African green monkey Kidney fibroblasts
	Bovine fetal fibroblast cells		Bovine ear-derived fibroblast cells
	Primary porcine dermal fibroblasts, kidney fibroblasts and myoblasts
	PAC2 zebrafish fibroblast cells		RTgutGC rainbow trout instetine cells

Cell Cultures (adherent electroporation)

	Primary human epidermal keratinocytes		Retinal Ganglion Cells from human iPSCs
	Primary mouse hippocampus and cortex		Primary mouse/rat cortical neurons
	Primary mouse Müller glia cells
	Primary mouse myoblasts		C2C12 mouse myotubes
	Primary rat DRG neurons		COS-7 African green monkey Kidney fibroblasts

Plant cells

Arabidopsis cells

Algae

	Diatom		Chlamydomonas
	Euglena		Nannochloropsis
	Coccomyxa		Closterium
	Bodo saltans

Yeasts

Schizosaccharomyces pombe

Bacterial cells

	Staphylococcus aureus		E. coli
	Corynebacterim glutamicum

Mouse/Rat in vivo

	Zygotes/pronuclear-stage embryos (TAKE)
	Embryos in oviduct (i-GONAD)
	Embryos in utero
	Embryos cultured
	Fertilized eggs & 8-16-cell stage embryos
	Endometrium
	Brain tissues ex vivo
	Brain neonatal
	Brain adult
	Retina in vivo/ex vivo
	Retina ex vivo
	Cornea
	Ear
	Sciatic Nerve
	Spinal Cord
	Spinal Cord ex vivo
	Lung
	Spleen
	Pancreas
	Islets of langerhans ex vivo
	Liver
	Kidney
	Colon
	Gonad ex vivo
	Prostate
	Testis
	Ovary
	Muscle
	Skin
	Joint
	Cartilage ex vivo
	Liver, Kidney, Spleen, Skin & Muscle
	Tumors

Human in vivo

Zygotes/Embryos

Monkey in vivo

	Zygotes/Embryos
	Skin

Bovine in vivo

Zygotes/Embryos

Pig/Sheep/Goat in vivo

Zygotes/Embryos

Hamster in vivo

Embryos in oviduct (i-GONAD)

Chicken in vivo

	Embryos in ovo
	Embryos ex ovo
	Retina ex vivo
	Brain in vivo

Zebrafish/Flounder in vivo

	Brain
	Retina in vivo/ex vivo
	Muscle
	Fin
	Embryos

Xenopus in vivo

	Blastema
	Embryos

Axolotls in vivo

	Blastema
	Limb or Dorsal root ganglion

Honey Bee in vivo

Brain

Cricket in vivo

	Brain
	Embryos

Water Flea in vivo

Water fleas

Planarian in vivo

Planarians

Butterfly in vivo

Larvae

Exosomes

Exosomes


Cell Cultures (suspension electroporation) Human iPS cells	Sayaka Deguchi, Tomohiro Shintani, Kazuo Harada,Toru Okamoto, Akinori Takemura, Kazumasa Hirata, Kousei Ito, Kazuo Takayama, Hiroyuki Mizuguchi In Vitro Model for a Drug Assessment of Cytochrome P450 Family 3 Subfamily A Member 4 Substrates Using Human Induced Pluripotent Stem Cells and Genome Editing Technology Hepatol. Commun. 05 May 2021. Tomoya Uchimura, Toshifumi Asano, Takao Nakata, Akitsu Hotta, Hidetoshi Sakurai A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs Cell Rep Med. 2021 Jun 4;2(6):100298. Akihiro Yamashita, Hiroyuki Yoshitomi, Shunsuke Kihara, Junya Toguchida, Noriyuki Tsumaki Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells Stem Cells Transl Med. 2021 Jan;10(1):115-127. Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao, Taisuke Tomita Autism-associated variants of neuroligin 4X impair synaptogenic activity by various molecular mechanisms Mol Autism. 2020 Sep 1;11(1):68. Ben Sasaki, Shinji Uemoto, Yoshiya Kawaguchi Transient FOXO1 inhibition in pancreatic endoderm promotes the generation of NGN3+ endocrine precursors from human iPSCs Stem Cell Res. 2020 Apr;44:101754. Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping Nat Commun. 2020 Mar 13;11(1):1334. Eul Sik Jung, Zhejiu Quan, Mi-Yoon Chang, Wonjun Hong, Ji Hun Kim, Seung Hyun Kim, Seungkwon You, Dae-Sung Kim, Jiho Jang, Sang-Hun Lee,Hyongbum (Henry) Kim, Hoon Chul Kang Successful Correction of ALD Patient-derived iPSCs Using CRISPR/Cas9 bioRxiv February 25, 2020 Margarete Diaz-Cuadros, Daniel E Wagner, Christoph Budjan, Alexis Hubaud, Oscar A Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourquié In Vitro Characterization of the Human Segmentation Clock Nature 2020 Jan 8[Online ahead of print] Takeshi Shiba, Takayuki Tanaka, Hiroaki Ida, Misa Watanabe, Haruna Nakaseko, Mitsujiro Osawa, Hirofumi Shibata, Kazushi Izawa, Takahiro Yasumi, Yuri Kawasaki, Megumu K Saito, Junko Takita, Toshio Heike, Ryuta Nishikomori Functional Evaluation of the Pathological Significance of MEFV Variants Using Induced Pluripotent Stem Cell-Derived Macrophages J Allergy Clin Immunol, 144 (5), 1438-1441.e12 Nov 2019 Shir Toubiana, Miriam Gagliardi, Mariarosaria Papa, Roberta Manco, Maty Tzukerman, Maria R Matarazzo, Sara Selig Persistent Epigenetic Memory Impedes Rescue of the Telomeric Phenotype in Human ICF iPSCs Following DNMT3B Correction Elife, 8 2019 Nov 20 Kanae Kawai, Ryosuke Negoro, Moe Ichikawa, Tomoki Yamashita, Sayaka Deguchi, Kazuo Harada, Kazumasa Hirata, Kazuo Takayama, Hiroyuki Mizuguchi Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies Mol Ther Methods Clin Dev, 17, 49-57 2019 Nov 21 eCollection 2020 Jun 12 Janin Grajcarek, Jean Monlong, Yoko Nishinaka-Arai, Michiko Nakamura, Miki Nagai, Shiori Matsuo, David Lougheed, Hidetoshi Sakurai, Megumu K Saito, Guillaume Bourque, Knut Woltjen Genome-wide Microhomologies Enable Precise Template-Free Editing of Biologically Relevant Deletion Mutations Nat Commun, 10 (1), 4856 2019 Oct 24 Takahiko Sato, Koki Higashioka, Hidetoshi Sakurai, Takuya Yamamoto, Naoki Goshima, Morio Ueno, Chie Sotozono Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells Stem Cell Reports, 13 (2), 352-365 2019 Aug 13 Sho Yoshimatsu, Takefumi Sone, Mayutaka Nakajima, Tsukika Sato, Ryotaro Okochi, Mitsuru Ishikawa, Mari Nakamura, Erika Sasaki, Seiji Shiozawa, Hideyuki Okano A Versatile Toolbox for Knock-In Gene Targeting Based on the Multisite Gateway Technology PLoS One, 14 (8), e0221164 2019 Aug 27 eCollection 2019 Daisuke Yoshinaga, Shiro Baba, Takeru Makiyama, Hirofumi Shibata, Takuya Hirata, Kentaro Akagi, Koichi Matsuda, Hirohiko Kohjitani, Yimin Wuriyanghai, Katsutsugu Umeda, Yuta Yamamoto, Bruce R Conklin, Minoru Horie, Junko Takita, Toshio Heike Phenotype-Based High-Throughput Classification of Long QT Syndrome Subtypes Using Human Induced Pluripotent Stem Cells Stem Cell Reports, 13 (2), 394-404 2019 Aug 13 Tetsuya Akiyama, Naoki Suzuki, Mitsuru Ishikawa, Koki Fujimori, Takefumi Sone, Jiro Kawada, Ryo Funayama, Fumiyoshi Fujishima, Shio Mitsuzawa, Kensuke Ikeda, Hiroya Ono, Tomomi Shijo, Shion Osana, Matsuyuki Shirota, Tadashi Nakagawa, Yasuo Kitajima, Ayumi Nishiyama, Rumiko Izumi, Satoru Morimoto, Yohei Okada, Takayuki Kamei, Mayumi Nishida, Masahiro Nogami, Shohei Kaneda, Yoshiho Ikeuchi, Hiroaki Mitsuhashi, Keiko Nakayama, Teruo Fujii, Hitoshi Warita, Hideyuki Okano, Masashi Aoki Aberrant Axon Branching via Fos-B Dysregulation in FUS-ALS Motor Neurons EBioMedicine, 45, 362-378 Jul 2019 Okada H, Nakanishi C, Yoshida S, Shimojima M, Yokawa J, Mori M, Tada H, Yoshimuta T, Hayashi K, Yamano T, Hanayama R, Yamagishi M, Kawashiri MA Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia. Sci Rep. 2019 Mar 18;9(1):4695. Ichisima J, Suzuki NM, Samata B, Awaya T, Takahashi J, Hagiwara M, Nakahata T, Saito MK Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model. J Hum Genet. 2019 Mar 8. Nakazeki F, Tsuge I, Horie T, Imamura K, Tsukita K, Hotta A, Baba O, Kuwabara Y, Nishino T, Nakao T, Nishiga M, Nishi H, Nakashima Y, Ide Y, Koyama S, Kimura M, Tsuji S, Naitoh M, Suzuki S, Izumi Y, Kawarai T, Kaji R, Kimura T, Inoue H, Ono K MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons. Clin Sci (Lond). 2019 Feb 22;133(4):583-595. Chizuru Iwasawa, Ryota Tamura, Yuki Sugiura, Sadafumi Suzuki, Naoko Kuzumaki, Minoru Narita, Makoto Suematsu, Masaya Nakamura, Kazunari Yoshida, Masahiro Toda, Hideyuki Okano, Hiroyuki Miyoshi Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells Int J Mol Sci, 20 (4) 2019 Feb 14 Minagawa A, Yoshikawa T, Yasukawa M, Hotta A, Kunitomo M, Iriguchi S, Takiguchi M, Kassai Y, Imai E, Yasui Y, Kawai Y, Zhang R, Uemura Y, Miyoshi H, Nakanishi M, Watanabe A, Hayashi A, Kawana K, Fujii T, Nakatsura T, Kaneko S Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy. Cell Stem Cell. 2018 Nov 1. pii: S1934-5909(18)30486-7. Tanigawa S, Islam M, Sharmin S, Naganuma H, Yoshimura Y, Haque F, Era T, Nakazato H, Nakanishi K, Sakuma T, Yamamoto T, Kurihara H, Taguchi A, Nishinakamura R Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes. Stem Cell Reports. 2018 Sep 11;11(3):727-740. Mitsuru Sasaki-Honda, Tatsuya Jonouchi, Meni Arai, Akitsu Hotta, Satomi Mitsuhashi, Ichizo Nishino, Ryoichi Matsuda, Hidetoshi Sakurai A Patient-Derived iPSC Model Revealed Oxidative Stress Increases Facioscapulohumeral Muscular Dystrophy-Causative DUX4 Hum Mol Genet, 27 (23), 4024-4035 2018 Dec 1 Tanaka Y, Sone T, Higurashi N, Sakuma T, Suzuki S, Ishikawa M, Yamamoto T, Mitsui J, Tsuji H, Okano H, Hirose S Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene. Stem Cell Res. 2018 Apr;28:100-104. Shin-Il Kim, Tomoko Matsumoto, Harunobu Kagawa, Michiko Nakamura, Ryoko Hirohata, Ayano Ueno, Maki Ohishi, Tetsushi Sakuma, Tomoyoshi Soga, Takashi Yamamoto, Knut Woltjen Microhomology-assisted Scarless Genome Editing in Human iPSCs Nat Commun, 9 (1), 939 2018 Mar 5 Yukari Suda, Naoko Kuzumaki, Takefumi Sone, Michiko Narita, Kenichi Tanaka, Yusuke Hamada, Chizuru Iwasawa, Masahiro Shibasaki, Aya Maekawa, Miri Matsuo, Wado Akamatsu, Nobutaka Hattori, Hideyuki Okano, Minoru Narita Down-regulation of Ghrelin Receptors on Dopaminergic Neurons in the Substantia Nigra Contributes to Parkinson's Disease-Like Motor Dysfunction Mol Brain, 11 (1), 6 2018 Feb 20 Takada S, Kambe N, Kawasaki Y, Niwa A, Honda-Ozaki F, Kobayashi K, Osawa M, Nagahashi A, Semi K, Hotta A, Asaka I, Yamada Y, Nishikomori R, Heike T, Matsue H, Nakahata T, Saito MK Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages. J Allergy Clin Immunol. 2018 Jan;141(1):339-349.e11. Kentaro Ishida, Huaigeng Xu, Noriko Sasakawa, Mandy Siu Yu Lung, Julia Alexandra Kudryashev, Peter Gee, Akitsu Hotta Site-specific Randomization of the Endogenous Genome by a Regulatable CRISPR-Cas9 piggyBac System in Human Cells Sci Rep, 8 (1), 310 2018 Jan 10 Linda Omer, Elizabeth A Hudson, Shirong Zheng, James B Hoying, Yuan Shan, Nolan L Boyd CRISPR Correction of a Homozygous Low-Density Lipoprotein Receptor Mutation in Familial Hypercholesterolemia Induced Pluripotent Stem Cells Hepatol Commun, 1 (9), 886-898 Nov 2017 Naohiro Horie, Atsuhiko Hikita, Satoru Nishizawa, Sakura Uto, Tsuyoshi Takato, Kazuto Hosha Impairment of the transition from proliferative stage to prehypertrophic stage in chondrogenic differentiation of human induced pluripotent stem cells harboring the causative mutation of achondroplasia in fibroblast growth factor receptor 3 Regenerative Therapy Volume 6, June 2017, Pages 15–20 Higashioka K, Koizumi N, Sakurai H, Sotozono C, Sato T Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9. Stem Cells Int. 2017;2017:9210494. Linda Omer, Elizabeth A Hudson, Jay B Hoying, Nolan L Boyd CRISPR/Cas9 Genome Editing to Repair Receptor-Mediated Endocytosis in Homozygous Familial Hypercholesterolemia Induced Pluripotent Stem Cells The FASEB Journal Vol. 31, 909.2, 1 Apr 2017 Nagahisa Murakami, Keiko Imamura, Yuishin Izumi, Naohiro Egawa, Kayoko Tsukita, Takako Enami, Takuya Yamamoto, Toshitaka Kawarai, Ryuji Kaji, Haruhisa Inoue Proteasome Impairment in Neural Cells Derived From HMSN-P Patient iPSCs Mol Brain, 10 (1), 7 2017 Feb 15 Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, Ueda HR, Ogawa K, Matsunaga T, Okano H Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 2017 Jan 3;18(1):68-81. Taizo Ishikawa, Keiko Imamura, Takayuki Kondo, Yasushi Koshiba, Satoshi Hara, Hiroshi Ichinose, Mahoko Furujo, Masako Kinoshita, Tomoko Oeda, Jun Takahashi, Ryosuke Takahashi, Haruhisa Inoue Genetic and Pharmacological Correction of Aberrant Dopamine Synthesis Using Patient iPSCs With BH4 Metabolism Disorders Hum Mol Genet, 25 (23), 5188-5197 2016 Dec 1 Keiko Imamura, Naruhiko Sahara, Nicholas M Kanaan, Kayoko Tsukita, Takayuki Kondo, Yumiko Kutoku, Yutaka Ohsawa, Yoshihide Sunada, Koichi Kawakami, Akitsu Hotta, Satoshi Yawata, Dai Watanabe, Masato Hasegawa, John Q Trojanowski, Virginia M-Y Lee, Tetsuya Suhara, Makoto Higuchi, Haruhisa Inoue Calcium Dysregulation Contributes to Neurodegeneration in FTLD Patient iPSC-derived Neurons Sci Rep, 6, 34904 2016 Oct 10 Sazia Sharmin, Atsuhiro Taguchi, Yusuke Kaku, Yasuhiro Yoshimura, Tomoko Ohmori, Tetsushi Sakuma, Masashi Mukoyama, Takashi Yamamoto, Hidetake Kurihara, Ryuichi Nishinakamura Human Induced Pluripotent Stem Cell-Derived Podocytes Mature Into Vascularized Glomeruli Upon Experimental Transplantation J Am Soc Nephrol, 27 (6), 1778-91 Jun 2016 Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol. 2016;1357:111-31. Morita Y, Andersen P, Hotta A, Tsukahara Y, Sasagawa N, Hayashida N, Koga C, Nishikawa M, Saga Y, Evans SM, Koshiba-Takeuchi K, Nishinakamura R, Yoshida Y, Kwon C, Takeuchi JK Sall1 transiently marks undifferentiated heart precursors and regulates their fate. J Mol Cell Cardiol. 2016 Mar;92:158-62. Li HL, Gee P, Ishida K, Hotta A Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system. Methods. 2015 Oct 23. pii: S1046-2023(15)30133-X. Hiromitsu Iizuka, Yuki Kagoya, Keisuke Kataoka, Akihide Yoshimi, Masashi Miyauchi, Kazuki Taoka, Keiki Kumano, Takashi Yamamoto, Akitsu Hotta, Shunya Arai, Mineo Kurokawa Targeted Gene Correction of RUNX1 in Induced Pluripotent Stem Cells Derived From Familial Platelet Disorder With Propensity to Myeloid Malignancy Restores Normal Megakaryopoiesis Exp Hematol, 43 (10), 849-57 Oct 2015 Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2015 Jan 13;4(1):143-54.
Reprogramming of iPS cells	Yutong Jing, Yalan Zhou, Congxiang Wang, Jian Liu, Yonglong Guo, Shengru Mao, Hon Fai Chan, Shibo Tang, Jiansu Chen Establishment of a Non-Integrate iPS Cell Line CSUASOi002-A, From Urine-Derived Cells of a Female Patient With Macular Corneal Dystrophy Carrying Compound Heterozygous CHST6 Mutations Stem Cell Res, 41, 101598 Dec 2019
Human ES cells	Namit Kumar, Yu-Hwai Tsai, Lei Chen, Anbo Zhou, Kushal K Banerjee, Madhurima Saxena, Sha Huang, Natalie H Toke, Jinchuan Xing, Ramesh A Shivdasani, Jason R Spence, Michael P Verzi The Lineage-Specific Transcription Factor CDX2 Navigates Dynamic Chromatin to Control Distinct Stages of Intestine Development Development, 146 (5) 2019 Mar 1 Karzbrun E, Kshirsagar A, Cohen SR, Hanna JH, Reiner O Human Brain Organoids on a Chip Reveal the Physics of Folding. Nat Phys. 2018 May;14(5):515-522. Wang XL, Yu L, Ding Y, Guo XR, Yuan YH, Li DS Gene Manipulation of Human Embryonic Stem Cells by In Vitro-Synthesized mRNA for Gene Therapy. Curr Gene Ther. 2015;15(4):428-35.
Human dissociated organoids	Maarten H Geurts, Shashank Gandhi, Matteo G Boretto, Ninouk Akkerman, Lucca L M Derks, Gijs van Son, Martina Celotti, Sarina Harshuk-Shabso, Flavia Peci, Harry Begthel, Delilah Hendriks, Paul Schürmann, Amanda Andersson-Rolf, Susana M Chuva de Sousa Lopes, Johan H van Es, Ruben van Boxtel, Hans Clevers One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids Nat Commun. 2023 Aug 17;14(1):4998. Rosemary Millen, Willem W B De Kort, Mandy Koomen, Gijs J F van Son, Roán Gobits, Bas Penning de Vries, Harry Begthel, Maurice Zandvliet, Patricia Doornaert, Cornelis P J Raaijmakers, Maarten H Geurts, Sjoerd G Elias, Robert J J van Es, Remco de Bree, Lot A Devriese, Stefan M Willems, Onno Kranenburg, Else Driehuis, Hans Clevers Patient-derived head and neck cancer organoids allow treatment stratification and serve as a tool for biomarker validation and identification Med. 2023 May 12;4(5):290-310.e12. Delilah Hendriks, Jos F Brouwers, Karien Hamer, Maarten H Geurts, Léa Luciana, Simone Massalini, Carmen López-Iglesias, Peter J Peters, Maria J Rodríguez-Colman, Susana Chuva de Sousa Lopes, Benedetta Artegiani, Hans Clevers Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis Nat Biotechnol. 2023 Feb 23. Yiyuan Niu, Catarina A Ferreira Azevedo, Xin Li, Elahe Kamali, Ole Haagen Nielsen, Claus Storgaard Sørensen, Morten Frödin Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select Nat Genet. 2022 Dec;54(12):1983-1993. Joep Beumer, Julia Bauzá-Martinez, Tim S Veth, Veerle Geurts, Charelle Boot, Hannah Gilliam-Vigh, Steen S Poulsen, Filip K Knop, Wei Wu, Hans Clevers Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models Proc Natl Acad Sci U S A. 2022 Nov 16;119(46):e2212057119. Carmen Aguilar, Mindaugas Pauzuolis, Malvika Pompaiah, Ehsan Vafadarnejad, Panagiota Arampatzi, Mara Fischer, Dominik Narres, Mastura Neyazi, Özge Kayisoglu, Thomas Sell, Nils Blüthgen, Markus Morkel, Armin Wiegering, Christoph-Thomas Germer, Stefan Kircher, Andreas Rosenwald, Antoine-Emmanuel Saliba, Sina Bartfeld Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxis Nat Commun. 2022 Oct 5;13(1):5878. Adriana Martinez-Silgado, Fjodor A Yousef Yengej, Jens Puschhof, Veerle Geurts, Charelle Boot, Maarten H Geurts, Maarten B Rookmaaker, Marianne C Verhaar, Joep Beumer, Hans Clevers Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids STAR Protoc. 2022 Aug 18;3(3):101639. Jessica K Nelson, May Zaw Thin, Theodore Evan, Steven Howell, Mary Wu, Bruna Almeida, Nathalie Legrave, Duco S Koenis, Gabriela Koifman, Yoichiro Sugimoto, Miriam Llorian Sopena, James MacRae, Emma Nye, Michael Howell, Ambrosius P Snijders, Andreas Prachalias, Yoh Zen, Debashis Sarker, Axel Behrens USP25 promotes pathological HIF-1-driven metabolic reprogramming and is a potential therapeutic target in pancreatic cancer Nat Commun. 2022 Apr 19;13(1):2070. I F Schene, I P Joore, J H L Baijens, R Stevelink, G Kok, S Shehata, E F Ilcken, E C M Nieuwenhuis, D P Bolhuis, R C M van Rees, S A Spelier, H P J van der Doef, J M Beekman, R H J Houwen, E E S Nieuwenhuis, S A Fuchs Mutation-specific reporter for optimization and enrichment of prime editing Nat Commun. 2022 Mar 1;13(1):1028. Joep Beumer, Jens Puschhof, Fjodor Yousef Yengej, Lianzheng Zhao, Adriana Martinez-Silgado, Marloes Blotenburg, Harry Begthel, Charelle Boot, Alexander van Oudenaarden, Ye-Guang Chen, Hans Clevers BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states Cell Rep. 2022 Mar 1;38(9):110438. Yannik Bollen, Joris H Hageman, Petra van Leenen, Lucca L M Derks, Bas Ponsioen, Julian R Buissant des Amorie, Ingrid Verlaan-Klink, Myrna van den Bos, Leon W M M Terstappen, Ruben van Boxtel, Hugo J G Snippert Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage PLoS Biol. 2022 Jan 28;20(1):e3001527. Sophie L Boos, Leon P Loevenich, Sebastian Vosberg, Thomas Engleitner, Rupert Öllinger, Jörg Kumbrink, Matjaz Rokavec, Marlies Michl, Philipp A Greif, Andreas Jung, Heiko Hermeking, Jens Neumann, Thomas Kirchner, Roland Rad, Peter Jung Disease Modeling on Tumor Organoids Implicates AURKA as a Therapeutic Target in Liver Metastatic Colorectal Cancer Cell Mol Gastroenterol Hepatol. 2022;13(2):517-540. Jelte van der Vaart, Lena Böttinger, Maarten H Geurts, Willine J van de Wetering, Kèvin Knoops, Norman Sachs, Harry Begthel, Jeroen Korving, Carmen Lopez-Iglesias, Peter J Peters, Kerem Eitan, Alex Gileles-Hillel, Hans Clevers Modelling of primary ciliary dyskinesia using patient-derived airway organoids EMBO Rep. 2021 Dec 6;22(12):e52058. Takuya Okamoto, Yasuko Natsume, Hitomi Yamanaka, Mayuko Fukuda, Ryoji Yao A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids STAR Protoc. 2021 Sep 16;2(4):100780. Maarten H Geurts, Eyleen de Poel, Cayetano Pleguezuelos-Manzano, Rurika Oka, Léo Carrillo, Amanda Andersson-Rolf, Matteo Boretto, Jesse E Brunsveld, Ruben van Boxtel, Jeffrey M Beekman, Hans Clevers Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids Life Sci Alliance. 2021 Aug 9;4(10):e202000940. Johanna F Dekkers, Esmée J van Vliet, Norman Sachs, Jennifer M Rosenbluth, Oded Kopper, Heggert G Rebel, Ellen J Wehrens, Carol Piani, Jane E Visvader, Carla S Verissimo, Sylvia F Boj, Joan S Brugge, Hans Clevers, Anne C Rios Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids Nat Protoc. 2021 Apr;16(4):1936-1965. Delilah Hendriks, Benedetta Artegiani, Huili Hu, Susana Chuva de Sousa Lopes, Hans Clevers Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver Nat Protoc. 2020 Nov 27 Maria C Heinz, Koen C Oost, Hugo J G Snippert Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids STAR Protoc. 2020 Oct 8;1(3):100126. Shinya Sugimoto, Masayuki Fujii, Toshiro Sato Organoid Derivation and Orthotopic Xenotransplantation for Studying Human Intestinal Stem Cell Dynamics Methods Mol Biol. 2020;2171:303-320. Benedetta Artegiani, Delilah Hendriks, Joep Beumer, Rutger Kok, Xuan Zheng, Indi Joore, Susana Chuva de Sousa Lopes, Jeroen van Zon, Sander Tans, Hans Clevers Fast and Efficient Generation of Knock-In Human Organoids Using Homology-Independent CRISPR-Cas9 Precision Genome Editing Nat Cell Biol 2020 Mar 2[Online ahead of print] Anne-Marlen Gaebler, Alexander Hennig, Katharina Buczolich, Jürgen Weitz, Thilo Welsch, Daniel E Stange, Kristin Pape Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids J Vis Exp. 2020 Feb 18;(156). Kenta Kawasaki, Masayuki Fujii, Shinya Sugimoto, Keiko Ishikawa, Mami Matano, Yuki Ohta, Kohta Toshimitsu, Sirirat Takahashi, Naoki Hosoe, Shigeki Sekine, Takanori Kanai, Toshiro Sato Chromosome Engineering of Human Colon-Derived Organoids to Develop a Model of Traditional Serrated Adenoma Gastroenterology, 158 (3), 638-651.e8 Feb 2020 Oded Kopper, Chris J de Witte, Kadi Lõhmussaar, Jose Espejo Valle-Inclan, Nizar Hami, Lennart Kester, Anjali Vanita Balgobind, Jeroen Korving, Natalie Proost, Harry Begthel, Lise M van Wijk, Sonia Aristín Revilla, Rebecca Theeuwsen, Marieke van de Ven, Markus J van Roosmalen, Bas Ponsioen, Victor W H Ho, Benjamin G Neel, Tjalling Bosse, Katja N Gaarenstroom, Harry Vrieling, Maaike P G Vreeswijk, Paul J van Diest, Petronella O Witteveen, Trudy Jonges, Johannes L Bos, Alexander van Oudenaarden, Ronald P Zweemer, Hugo J G Snippert, Wigard P Kloosterman, Hans Clevers An Organoid Platform for Ovarian Cancer Captures Intra- And Interpatient Heterogeneity Nat Med, 25 (5), 838-849 May 2019 Maureen Spit, Nicola Fenderico, Ingrid Jordens, Tomasz Radaszkiewicz, Rik G.H. Lindeboom, Jeroen M. Bugter, Lars Ootes, Max Van Osch, Eline Janssen, Kim E. Boonekamp, Katerina Hanakova, David Potesil, Zbynek Zdrahal, Sylvia F. Boj, Jan Paul Medema, Vitezslav Bryja, Bon-Kyoung Koo, Michiel Vermeulen, Madelon M. Maurice RNF43 truncating mutations mediate a tumour suppressor-to-oncogene switch to drive niche-independent self-renewal in cancer bioRxiv September 01, 2019. Fujii M, Matano M, Toshimitsu K, Takano A, Mikami Y, Nishikori S, Sugimoto S, Sato T Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition. Cell Stem Cell. 2018 Dec 6;23(6):787-793.e6. Park YL, Kim HP, Cho YW, Min DW, Cheon SK, Lim YJ, Song SH, Jin Kim S, Han SW, Park KJ, Kim TY Activation of WNT/β-catenin signaling results in resistance to a dual PI3K/mTOR inhibitor in colorectal cancer cells harboring PIK3CA mutations. Int J Cancer. 2018 Jul 6. doi: 10.1002/ijc.31662. [Epub ahead of print] Kosuke Kirino, Tatsutoshi Nakahata, Tomoaki Taguchi, Megumu K Saito Efficient Derivation of Sympathetic Neurons From Human Pluripotent Stem Cells With a Defined Condition Sci Rep, 8 (1), 12865 2018 Aug 27 van Rijn JM, Ardy RC, Kuloğlu Z, Härter B, van Haaften-Visser DY, van der Doef HPJ, van Hoesel M, Kansu A, van Vugt AHM, Thian M, Kokke FTM, Krolo A, Başaran MK, Kaya NG, Aksu AÜ, Dalgıç B, Ozcay F, Baris Z, Kain R, Stigter ECA, Lichtenbelt KD, Massink MPG, Duran KJ, Verheij JBGM, Lugtenberg D, Nikkels PGJ, Brouwer HGF, Verkade HJ, Scheenstra R, Spee B, Nieuwenhuis EES, Coffer PJ, Janecke AR, van Haaften G, Houwen RHJ, Müller T, Middendorp S, Boztug K Intestinal Failure and Aberrant Lipid Metabolism in Patients With DGAT1 Deficiency. Gastroenterology. 2018 Jul;155(1):130-143.e15. Sachs N, de Ligt J, Kopper O, Gogola E, Bounova G, Weeber F, Balgobind AV, Wind K, Gracanin A, Begthel H, Korving J, van Boxtel R, Duarte AA, Lelieveld D, van Hoeck A, Ernst RF, Blokzijl F, Nijman IJ, Hoogstraat M, van de Ven M, Egan DA, Zinzalla V, Moll J, Boj SF, Voest EE, Wessels L, van Diest PJ, Rottenberg S, Vries RGJ, Cuppen E, Clevers H. A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. Cell. 2018 Jan 11;172(1-2):373-386.e10. Uchida H, Machida M, Miura T, Kawasaki T, Okazaki T, Sasaki K, Sakamoto S, Ohuchi N, Kasahara M, Umezawa A, Akutsu H A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells. JCI Insight. 2017 Jan 12;2(1):e86492. Fujii M, Matano M, Nanki K, Sato T Efficient genetic engineering of human intestinal organoids using electroporation. Nat Protoc. 2015 Oct;10(10):1474-85. Epub 2015 Sep 3. Matano M, Date S, Shimokawa M, Takano A, Fujii M, Ohta Y, Watanabe T, Kanai T, Sato T. Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. Nat Med. 2015 Mar;21(3):256-62.
Human organoids	Benedetta ADelilah Hendriks, Benedetta Artegiani, Huili Hu, Susana Chuva de Sousa Lopes, Hans Clevers Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver Nat Protoc. 2020 Nov 27 rtegiani, Lisa van Voorthuijsen, Rik G H Lindeboom, Daniëlle Seinstra, Inha Heo, Pablo Tapia, Carmen López-Iglesias, Daniel Postrach, Talya Dayton, Rurika Oka, Huili Hu, Ruben van Boxtel, Johan H van Es, Johan Offerhaus, Peter J Peters, Jacco van Rheenen, Michiel Vermeulen, Hans Clevers Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids Cell Stem Cell, 24 (6), 927-943.e6 2019 Jun 6
Cancer tissue- originated spheroids	Satoshi Kubota, Mie Tanaka, Hiroko Endo, Yu Ito, Kunishige Onuma, Yutaka Ueda, Shoji Kamiura, Kiyoshi Yoshino, Tadashi Kimura, Jumpei Kondo, Masahiro Inoue Dedifferentiation of neuroendocrine carcinoma of the uterine cervix in hypoxia Biochem Biophys Res Commun. 2020 Apr 2;524(2):398-404.
Human adipose mesenchymal stem cells (hAMSCs)	Meca-Cortés O, Guerra-Rebollo M, Garrido C, Borrós S, Rubio N, Blanco J CRISPR/Cas9-Mediated Knockin Application in Cell Therapy: A Non-viral Procedure for Bystander Treatment of Glioma in Mice. Mol Ther Nucleic Acids. 2017 Sep 15;8:395-403.
Human dental pulp stem cells (DPSCs)	Kentaro Nonaka, Xu Han, Hiroki Kato, Hiroshi Sato, Haruyoshi Yamaza, Yuta Hirofuji, Keiji Masuda Novel Gain-Of-Function Mutation of TRPV4 Associated With Accelerated Chondrogenic Differentiation of Dental Pulp Stem Cells Derived From a Patient With Metatropic Dysplasia Biochem Biophys Rep, 19, 100648 2019 May 17
Primary human skin fibroblasts	Lesport E, Ferster A, Biver A, Roch B, Vasquez N, Jabado N, Vives FL, Revy P, Soulier J, de Villartay JP Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia. Oncotarget. 2017 Dec 17;9(3):3779-3793. Dunwell TL, Holland PWH A sister of NANOG regulates genes expressed in pre-implantation human development. Open Biol. 2017 Apr;7(4). pii: 170027. Maeso I, Dunwell TL, Wyatt CD, Marlétaz F, Vető B, Bernal JA, Quah S, Irimia M, Holland PW Evolutionary origin and functional divergence of totipotent cell homeobox genes in eutherian mammals. BMC Biol. 2016 Jun 13;14:45.
Primary human hepatocytes	Hironori Bando, Ariel Pradipta, Shiroh Iwanaga, Toru Okamoto, Daisuke Okuzaki, Shun Tanaka, Joel Vega-Rodríguez, Youngae Lee, Ji Su Ma, Naoya Sakaguchi, Akira Soga, Shinya Fukumoto, Miwa Sasai, Yoshiharu Matsuura, Masao Yuda, Marcelo Jacobs-Lorena, Masahiro Yamamoto CXCR4 Regulates Plasmodium Development in Mouse and Human Hepatocytes J Exp Med. 2019 Aug 5;216(8):1733-1748.
PBMC	Shibaguchi H, Luo N, Shirasu N, Kuroki M, Kuroki M Enhancement of antitumor activity by using a fully human gene encoding a single-chain fragmented antibody specific for carcinoembryonic antigen. Onco Targets Ther. 2017 Aug 22;10:3979-3990.
Primary human T cells	Toshiaki Yoshikawa, Zhiwen Wu, Satoshi Inoue, Hitomi Kasuya, Hirokazu Matsushita, Yusuke Takahashi, Hiroaki Kuroda, Waki Hosoda, Shiro Suzuki, Yuki Kagoya Genetic ablation of PRDM1 in antitumor T cells enhances therapeutic efficacy of adoptive immunotherapy Blood. 2021 Dec 3;blood.2021012714. Zhendong Li, Zhixia Chi, Wei-Xia Ang, Can Chen, Johan Ck Tay, Yu-Yang Ng, Xuehu Xu, Junjian Wang, Jianqing Zhu, Shu Wang Experimental treatment of colorectal cancer in mice with human T cells electroporated with NKG2D RNA CAR Immunotherapy. 2020 Jul;12(10):733-748. Xiao L, Chen C, Li Z, Zhu S, Tay JC, Zhang X, Zha S, Zeng J, Tan WK, Liu X, Chng WJ, Wang S Large-scale expansion of Vγ9Vδ2 T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor-modified effector cells. Cytotherapy. 2018 Mar;20(3):420-435. Shibaguchi H, Luo N, Shirasu N, Kuroki M, Kuroki M Enhancement of antitumor activity by using a fully human gene encoding a single-chain fragmented antibody specific for carcinoembryonic antigen. Onco Targets Ther. 2017 Aug 22;10:3979-3990. Ang WX, Li Z, Chi Z, Du SH, Chen C, Tay JC1, Toh HC, Connolly JE, Xu XH, Wang S Intraperitoneal immunotherapy with T cells stably and transiently expressing anti-EpCAM CAR in xenograft models of peritoneal carcinomatosis. Oncotarget. 2017 Feb 21;8(8):13545-13559. Du SH, Li Z, Chen C, Tan WK, Chi Z, Kwang TW, Xu XH, Wang S Co-Expansion of Cytokine-Induced Killer Cells and Vγ9Vδ2 T Cells for CAR T-Cell Therapy. PLoS One. 2016 Sep 6;11(9):e0161820.
Primary human NK cells	Yu Yang Ng, Johan C K Tay, Shu Wang CXCR1 Expression to Improve Anti-Cancer Efficacy of Intravenously Injected CAR-NK Cells in Mice With Peritoneal Xenografts Mol Ther Oncolytics, 16, 75-85 2019 Dec 24 Lin Xiao, Dongzhi Cen, Haining Gan, Yan Sun, Nanqi Huang, Hanzhen Xiong, Qiongmei Jin, Liqun Su, Xuejuan Liu, Kejian Wang, Guangrong Yan, Tianfa Dong, Shangbiao Wu, Pengzhi Zhou, Jinshan Zhang, Weixiang Liang, Junlan Ren, Yaoshu Teng, Can Chen, Xue Hu Xu Adoptive Transfer of NKG2D CAR mRNA-Engineered Natural Killer Cells in Colorectal Cancer Patients Mol Ther, 27 (6), 1114-1125 2019 Jun 5
Primary human B cells	Federica Nardi, Laura Pezzella, Roberta Drago, Anthea Di Rita, Martina Simoncelli, Giuseppe Marotta, Alessandro Gozzetti, Monica Bocchia, Anna Kabanovag Assessing gene function in human B cells: CRISPR/Cas9-based gene editing and mRNA-based gene expression in healthy and tumor cells Eur J Immunol. 2022 Aug;52(8):1362-1365.
Primary Human airway epithelial basal cells	Lisa W Rodenburg, Livia Delpiano, Violeta Railean, Raquel Centeio, Madalena C Pinto, Shannon M A Smits, Isabelle S van der Windt, Casper F J van Hugten, Sam F B van Beuningen, Remco N P Rodenburg, Cornelis K van der Ent, Margarida D Amaral, Karl Kunzelmann, Michael A Gray, Jeffrey M Beekman, Gimano D Amatngalim Drug Repurposing for Cystic Fibrosis: Identification of Drugs That Induce CFTR-Independent Fluid Secretion in Nasal Organoids Int J Mol Sci. 2022 Oct 21;23(20):12657.
HEK293	Tomohiro Kotaki, Xuping Xie, Pei-Yong Shi, Masanori Kameoka A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation Sci Rep. 2021 Jan 26;11(1):2229. Riho Kurata, Kenji Shimizu, Xiaofeng Cui, Masamitsu Harada, Takayuki Isagawa, Hiroaki Semba, Jun Ishihara, Koji Yamada, Jun Nagai, Yasuhiro Yoshida, Norihiko Takeda, Koji Maemura, Tomo Yonezawa Novel Reporter System Monitoring IL-18 Specific Signaling Can Be Applied to High-Throughput Screening Mar Drugs. 2020 Jan 17;18(1):60. Kurata R, Kumagai A, Cui X, Harada M, Nagai J3, Yoshida Y, Ozaki KI, Tanaka Y, Yonezawa T Establishment of Novel Reporter Cells Stably Maintaining Transcription Factor-driven Human Secreted Alkaline Phosphatase Expression. Curr Pharm Biotechnol. 2018;19(3):224-231. Hirose M, Hasegawa A, Mochida K, Matoba S, Hatanaka Y, Inoue K, Goto T, Kaneda H, Yamada I, Furuse T, Abe K, Uenoyama Y, Tsukamura H, Wakana S, Honda A, Ogura A CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene. Sci Rep. 2017 Feb 14;7:42476.
TIG-7 HeLa	Kobayashi Y, Lee SS, Arai R, Miki K, Fujii M, Ayusawa D ERK1/2 mediates unbalanced growth leading to senescence induced by excess thymidine in human cells. Biochem Biophys Res Commun. 2012 Sep 7;425(4):897-901
U251	Fu R, Ding Y, Luo J, Yu L, Li CL, Li DS, Guo SW. TET1 exerts its tumor suppressor function by regulating autophagy in glioma cells. Biosci Rep. 2017 Mar 24. pii: BSR20160523.
HCS-2/8	Hattori Y, Kojima T, Kato D, Matsubara H, Takigawa M, Ishiguro N. A selective estrogen receptor modulator inhibits tumor necrosis factor-α-induced apoptosis through the ERK1/2 signaling pathway in human chondrocytes. Biochem Biophys Res Commun. 2012 May 11;421(3):418-24.
Jurkat	Irini Evnouchidou, Pascal Chappert, Samira Benadda, Andres Zucchetti, Mirjana Weimershaus, Marcelle Bens, Vivien Caillens, Despoina Koumantou, Sophie Lotersztajn, Peter van Endert, Jean Davoust, Pierre Guermonprez, Claire Hivroz, David A Gross, Loredana Saveanu IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses Nat Commun. 2020 Jun 2;11(1):2779. Emmanuel Martin, Norbert Minet, Anne-Claire Boschat, Sylvia Sanquer, Steicy Sobrino, Christelle Lenoir, Jean Pierre de Villartay, Maria Leite-de-Moraes, Capucine Picard, Claire Soudais, Tim Bourne, Sophie Hambleton, Stephen M Hughes, Robert F Wynn, Tracy A Briggs, Genomics England Research Consortium; Smita Patel, Monica G Lawrence, Alain Fischer, Peter D Arkwright, Sylvain Latour Impaired Lymphocyte Function and Differentiation in CTPS1-deficient Patients Result From a Hypomorphic Homozygous Mutation JCI Insight. 2020 Mar 12;5(5):e133880. Matsubara Y, Chiba T, Kashimada K, Morio T, Takada S, Mizutani S, Asahara H Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus. Sci Rep. 2014 May 23;4:5043. Yamada N, Noguchi S, Kumazaki M, Shinohara H, Miki K, Naoe T, Akao Y Epigenetic regulation of microRNA-128a expression contributes to the apoptosis-resistance of human T-cell leukaemia jurkat cells by modulating expression of fas-associated protein with death domain (FADD). Biochim Biophys Acta. 2014 Mar;1843(3):590-602.
ME-1 A-E/CD34+ preleukemic cells	Ben-Ami O, Friedman D, Leshkowitz D, Goldenberg D, Orlovsky K, Pencovich N, Lotem J, Tanay A, Groner Y. Addiction of t(8;21) and inv(16) acute myeloid leukemia to native RUNX1. Cell Rep. 2013 Sep 26;4(6):1131-43.
CTL Cytotoxic T Lympocytes	Ben-Lucine Marotte, Sylvain Simon, Virginie Vignard, Emilie Dupre, Malika Gantier, Jonathan Cruard, Jean-Baptiste Alberge, Melanie Hussong, Cecile Deleine, Jean-Marie Heslan, Jonathan Shaffer, Tiffany Beauvais, Joelle Gaschet, Emmanuel Scotet, Delphine Fradin, Anne Jarry, Tuan Nguyen, Nathalie Labarriere Increased Antitumor Efficacy of PD-1-deficient Melanoma-Specific Human Lymphocytes J Immunother Cancer. 2020 Jan;8(1):e000311.
HeLa	Tsuchiya H, Burana D, Ohtake F, Arai N, Kaiho A, Komada M, Tanaka K, Saeki Y Ub-ProT reveals global length and composition of protein ubiquitylation in cells. Nat Commun. 2018 Feb 6;9(1):524. Yuya Hikone, Go Hirai, Masaki Mishima, Kohsuke Inomata, Teppei Ikeya, Souichiro Arai, Masahiro Shirakawa, Mikiko Sodeoka, Yutaka Ito A New Carbamidemethyl-Linked Lanthanoid Chelating Tag for PCS NMR Spectroscopy of Proteins in Living HeLa Cells J Biomol NMR. 2016 Oct;66(2):99-110.
PC-3	Takanori Eguchi, Chiharu Sogawa, Kisho Ono, Masaki Matsumoto, Manh Tien Tran, Yuka Okusha, Benjamin J Lang, Kuniaki Okamoto, Stuart K Calderwood Cell Stress Induced Stressome Release Including Damaged Membrane Vesicles and Extracellular HSP90 by Prostate Cancer Cells Cells. 2020 Mar 19;9(3):755.
PLB-985	Masahiro Maruoka, Panpan Zhang, Hiromi Mori, Eiichi Imanishi, Daniel M Packwood, Hiroshi Harada, Hidetaka Kosako, Jun Suzuki Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane Mol Cell. 2021 Apr 1;81(7):1397-1410.e9.
Mouse ES cells	Lucas Kissling, Asun Monfort, Daan C Swarts, Anton Wutz, Martin Jinek Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells Methods Enzymol. 2019;616:241-263. Takashi Serizawa, Ayako Isotani, Takafumi Matsumura, Katsuyuki Nakanishi, Shigenori Nonaka, Shinsuke Shibata, Masahito Ikawa, Hideyuki Okano Developmental Analyses of Mouse Embryos and Adults Using a Non-Overlapping Tracing System for All Three Germ Layers Development, 146 (21) 2019 Nov 4 Kissling L, Monfort A, Swarts DC, Wutz A, Jinek M Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells. Methods Enzymol. 2019;616:241-263.
Mouse dissociated organoids	Marie Bannier-Hélaouët, Maarten H Geurts, Jeroen Korving, Harry Begthel, Hans Clevers Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids J Vis Exp. 2023 Feb 3;(192). Linxiang Lan, Theodore Evan, Huafu Li, Aasia Hussain, E Josue Ruiz, May Zaw Thin, Rute M M Ferreira, Hari Ps, Eva M Riising, Yoh Zen, Jorge Almagro, Kevin W Ng, Pablo Soro-Barrio, Jessica Nelson, Gabriela Koifman, Joana Carvalho, Emma L Nye, Yulong He, Changhua Zhang, Anguraj Sadanandam, Axel Behrens GREM1 is required to maintain cellular heterogeneity in pancreatic cancer Nature. 2022 Jul;607(7917):163-168. Peter M K Westcott, Nathan J Sacks, Jason M Schenkel, Zackery A Ely, Olivia Smith, Haley Hauck, Alex M Jaeger, Daniel Zhang, Coralie M Backlund, Mary C Beytagh, J J Patten, Ryan Elbashir, George Eng, Darrell J Irvine, Omer H Yilmaz, Tyler Jacks Low neoantigen expression and poor T-cell priming underlie early immune escape in colorectal cancer Nat Cancer. 2021 Oct;2(10):1071-1085. Marie Bannier-Hélaouët, Yorick Post, Jeroen Korving, Marc Trani Bustos, Helmuth Gehart, Harry Begthel, Yotam E Bar-Ephraim, Jelte van der Vaart, Rachel Kalmann, Saskia M Imhoff, Hans Clevers Exploring the human lacrimal gland using organoids and single-cell sequencing Cell Stem Cell. 2021 Jul 1;28(7):1221-1232.e7. Merenda A, Andersson-Rolf A, Mustata RC, Li T, Kim H, Koo BK A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. J Vis Exp. 2017 Jul 12;(125).
Primary mouse neurons	Huang B, Li X, Tu X, Zhao W, Zhu D, Feng Y, Si X, Chen JG OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex. J Biol Chem. 2018 Feb 9;293(6):2137-2148. Shioda N, Yabuki Y, Wang Y, Uchigashima M, Hikida T, Sasaoka T, Mori H, Watanabe M, Sasahara M, Fukunaga K Endocytosis following dopamine D2 receptor activation is critical for neuronal activity and dendritic spine formation via Rabex-5/PDGFRβ signaling in striatopallidal medium spiny neurons. Mol Psychiatry. 2017 Aug;22(8):1205-1222. Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O. Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons. J Neurosci. 2013 Jul 17;33(29):11932-48. Kusuzawa S, Honda T, Fukata Y, Fukata M, Kanatani S, Tanaka DH, Nakajima K. Leucine-rich glioma inactivated 1 (Lgi1), an epilepsy-related secreted protein, has a nuclear localization signal and localizes to both the cytoplasm and the nucleus of the caudal ganglionic eminence neurons. Eur J Neurosci. 2012 Aug;36(3):2284-92.
Primary mouse astrocytes	Ishii T, Ueyama T, Shigyo M, Kohta M, Kondoh T, Kuboyama T, Uebi T, Hamada T, Gutmann DH, Aiba A, Kohmura E, Tohda C, Saito N A Novel Rac1-GSPT1 Signaling Pathway Controls Astrogliosis Following Central Nervous System Injury. J Biol Chem. 2017 Jan 27;292(4):1240-1250.
Primary mouse bone marrow- derived mast cells (BMMCs)	Horiguchi K, Yoshikawa S, Saito A, Haddad S, Ohta T, Miyake K, Yamanishi Y, Karasuyama H Real-time imaging of mast cell degranulation in vitro and in vivo. Biochem Biophys Res Commun. 2016 Oct 21;479(3):517-522.
Primary mouse hematopoietic stem cells (HSCs)	HoSuvd Byambaa, Hideki Uosaki, Tsukasa Ohmori, Hiromasa Hara, Hitoshi Endo, Osamu Nureki, Yutaka Hanazono Non-viral ex vivo genome-editing in mouse bona fide hematopoietic stem cells with CRISPR/Cas9. Mol Ther Methods Clin Dev. 2021 Jan 9:20:451-462.
Ba/F3	Takaharu Sakuragi, Ryuta Kanai, Akihisa Tsutsumi, Hirotaka Narita, Eriko Onishi, Kohei Nishino, Takuya Miyazaki, Takeshi Baba, Hidetaka Kosako, Atsushi Nakagawa, Masahide Kikkawa, Chikashi Toyoshima, Shigekazu Nagata The tertiary structure of the human Xkr8-Basigin complex that scrambles phospholipids at plasma membranes Nat Struct Mol Biol. 2021 Oct;28(10):825-834. Masahiro Maruoka, Panpan Zhang, Hiromi Mori, Eiichi Imanishi, Daniel M Packwood, Hiroshi Harada, Hidetaka Kosako, Jun Suzuki Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane Mol Cell. 2021 Apr 1;81(7):1397-1410.e9. Takaharu Sakuragi, Hidetaka Kosako, Shigekazu Nagata Phosphorylation-mediated activation of mouse Xkr8 scramblase for phosphatidylserine exposure Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):2907-2912. Takuya Hirao, Masashi Yamaguchi, Megumi Kikuya, Hiroji Chiban, Kousei Ito, Shigeki Aoki Altered intracellular signaling by imatinib increases the anti-cancer effects of tyrosine kinase inhibitors in chronic myelogenous leukemia cells Cancer Sci. 2018 Jan;109(1):121-131. Nishi C, Toda S, Segawa K, Nagata S. Tim4- and MerTK-mediated engulfment of apoptotic cells by mouse resident peritoneal macrophages. Mol Cell Biol. 2014 Apr;34(8):1512-20.
C2C12	Guo Q, Hu H, Zhou Y, Yan Y, Wei X, Fan X, Yang D, He H, Oh Y, Chen K, Wu Q, Liu C, Gu N Glucosamine induces increased musclin gene expression through endoplasmic reticulum stress-induced unfolding protein response signaling pathways in mouse skeletal muscle cells. Food Chem Toxicol. 2019 Mar;125:95-105. Ohkawara B, Cabrera-Serrano M, Nakata T, Milone M, Asai N, Ito K, Ito M, Masuda A, Ito Y, Engel AG, Ohno K LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet. 2013 Nov 29. [Epub ahead of print]
MEF 3T3-L1	Liu B1, Wang T, Mei W, Li D, Cai R, Zuo Y, Cheng J SUMO-Specific Protease 1 De-SUMOylates Sharp-1 and Controls Adipocyte Differentiation. J Biol Chem. 2014 Jun 18.
3T3-L1	Ishijima Y, Ohmori S, Uneme A, Aoki Y, Kobori M, Ohida T, Arai M, Hosaka M, Ohneda K The Gata2 repression during 3T3-L1 preadipocyte differentiation is dependent on a rapid decrease in histone acetylation in response to glucocorticoid receptor activation. Mol Cell Endocrinol. 2019 Mar 1;483:39-49.
NIH/3T3	Ikeda E, Matsunaga N, Kakimoto K, Hamamura K, Hayashi A, Koyanagi S, Ohdo S. Molecular mechanism regulating 24-hour rhythm of dopamine D3 receptor expression in mouse ventral striatum. Mol Pharmacol. 2013 May;83(5):959-67.
RAW264.7	Shirakabe K, Hattori S, Seiki M, Koyasu S, Okada Y. VIP36 protein is a target of ectodomain shedding and regulates phagocytosis in macrophage Raw 264.7 cells. J Biol Chem. 2011 Dec 16;286(50):43154-63.
ZS mouse embryonic fibroblasts	Hayashi Y, Nemoto-Sasaki Y, Tanikawa T, Oka S, Tsuchiya K, Zama K, Mitsutake S, Sugiura T, Yamashita A. Sphingomyelin synthase 2, but not sphingomyelin synthase 1, is involved in HIV-1 envelope-mediated membrane fusion. J Biol Chem. 2014 Oct 31;289(44):30842-56.
Hepa1-6 mouse hepatoma cells	Yuhei Mizunoe, Masaki Kobayashi, Yuka Sudo, Shukoh Watanabe, Hiromine Yasukawa, Daiki Natori, Ayana Hoshino, Arisa Negishi, Naoyuki Okita, Masaaki Komatsu, Yoshikazu Higami Trehalose Protects Against Oxidative Stress by Regulating the Keap1-Nrf2 and Autophagy Pathways Redox Biol. 2018 May;15:115-124.
Primary rat neurons	Natalia Bal, Aleksey Malyshev, Ivan Smirnov, Pavel Balaban Expression of Channelrhodopsin-2 Using in Suspension Electroporation for Studying the Monosynaptic Transmission in Neuronal Culture BioNanoScience 2016 Dec;6(4):329-331 Zhang C, Zhang Z, Zhao Q, Wang X, Ji H, Zhang Y. (S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation. Neurotox Res. 2013 Nov 26. [Epub ahead of print]
RSC96	Gui T, Wang Y, Zhang L, Wang W, Zhu H, Ding W. Krüppel-like factor 6 rendered rat Schwann cell more sensitive to apoptosis via upregulating FAS expression. PLoS One. 2013 Dec 4;8(12):e82449.
Rat hair follicle stem cells	Li X1, Wu Y, Xie F, Zhang F, Zhang S, Zhou J, Chen D, Liu A miR‑339‑5p negatively regulates loureirin A‑induced hair follicle stem cell differentiation by targeting DLX5. Mol Med Rep. 2018 Aug;18(2):1279-1286.
Rat dermal fibroblast sheets	Muraoka I, Takatsuki M, Sakai Y, Tomonaga T, Soyama A, Hidaka M, Hishikawa Y, Koji T, Utoh R, Ohashi K, Okano T, Kanematsu T, Eguchi S. Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model. J Tissue Eng Regen Med. 2013 Mar 12.
Shrew spermatogonial stem cells (SSCs)	Chao-Hui Li, Lan-Zhen Yan, Wen-Zan Ban, Qiu Tu, Yong Wu, Lin Wang, Rui Bi, Shuang Ji, Yu-Hua Ma, Wen-Hui Nie, Long-Bao Lv, Yong-Gang Yao, Xu-Dong Zhao, Ping Zheng Long-term Propagation of Tree Shrew Spermatogonial Stem Cells in Culture and Successful Generation of Transgenic Offspring Cell Res, 27 (2), 241-252 Feb 2017
CHO	Tomohiro Kotaki, Xuping Xie, Pei-Yong Shi, Masanori Kameoka A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation Sci Rep. 2021 Jan 26;11(1):2229. Sung Wook Shin, Jae Seong Lee Optimized CRISPR/Cas9 Strategy for Homology-Directed Multiple Targeted Integration of Transgenes in CHO Cells Biotechnol Bioeng 2020 Feb 22 [Online ahead of print] Po-Kuan Chao, Hsiao-Fu Chang, Li-Chin Ou, Jian-Ying Chuang, Pin-Tse Lee, Wan-Ting Chang, Shu-Chun Chen, Shau-Hua Ueng, John Tsu-An Hsu, Pao-Luh Tao, Ping-Yee Law, Horace H Loh, Shiu-Hwa Yeh Convallatoxin Enhance the Ligand-Induced Mu-Opioid Receptor Endocytosis and Attenuate Morphine Antinociceptive Tolerance in Mice Sci Rep, 9 (1), 2405 2019 Feb 20 Rie Hatanaka, Oleg Gusev, Richard Cornette, Sachiko Shimura, Shingo Kikuta, Jun Okada, Takashi Okuda, Takahiro Kikawada Diversity of the Expression Profiles of Late Embryogenesis Abundant (LEA) Protein Encoding Genes in the Anhydrobiotic Midge Polypedilum Vanderplanki Planta, 242 (2), 451-9 Aug 2015
Chick spinal cord neurons	Sahasrabudhe A, Ghate K, Mutalik S, Jacob A, Ghose A Formin-2 regulates stabilization of filopodial tip adhesions in growth cones and affects neuronal outgrowth and pathfinding in vivo. Development. 2015 Dec 30. pii: dev.130104. [Epub ahead of print]
MDCK MDCK II	Sato Y, Akitsu M, Amano Y, Yamashita K, Ide M, Shimada K, Yamashita A, Hirano H, Arakawa N, Maki T, Hayashi I, Ohno S, Suzuki A. The novel PAR-1-binding protein MTCL1 has crucial roles in organizing microtubules in polarizing epithelial cells. J Cell Sci. 2013 Oct 15;126(Pt 20):4671-83.
Primary cynomolgus PBMCs	Hirokazu Morokawa, Shigeki Yagyu, Aiko Hasegawa, Miyuki Tanaka, Shoji Saito, Hidemi Mochizuki, Kengo Sakamoto, Akihito Shimoi, Yozo Nakazawa Autologous non-human primate model for safety assessment of piggyBac transposon-mediated chimeric antigen receptor T cells on granulocyte-macrophage colony-stimulating factor receptor Clin Transl Immunology. 2020 Nov 22;9(11):e1207.
Primary rhesus macaque CD4+ T cells	Wang X, Yu Q, Yuan Y, Teng Z3, Li D, Zeng Y Targeting the rhesus macaque TRIM5α gene to enhance the susceptibility of CD4+ T cells to HIV-1 infection. Arch Virol. 2017 Mar;162(3):793-798.
Primary rhesus macaque CD4+ T cells	Wang X, Yu Q, Yuan Y, Teng Z3, Li D, Zeng Y Targeting the rhesus macaque TRIM5α gene to enhance the susceptibility of CD4+ T cells to HIV-1 infection. Arch Virol. 2017 Mar;162(3):793-798.
COS-7	Masahiro Maruoka, Panpan Zhang, Hiromi Mori, Eiichi Imanishi, Daniel M Packwood, Hiroshi Harada, Hidetaka Kosako, Jun Suzuki Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane Mol Cell. 2021 Apr 1;81(7):1397-1410.e9.
Bovine fetal fibroblast cells	Ikeda M, Matsuyama S, Akagi S, Ohkoshi K, Nakamura S, Minabe S, Kimura K, Hosoe M Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle. Sci Rep. 2017 Dec 19;7(1):17827.
Bovine ear-derived fibroblast cells	Ishino T, Hashimoto M, Amagasa M, Saito N, Dochi O, Kirisawa R, Kitamura H Establishment of protocol for preparation of gene-edited bovine ear-derived fibroblasts for somatic cell nuclear transplantation. Biomed Res. 2018;39(2):95-104.
Primary porcine dermal fibroblasts, kidney fibroblasts, and myoblasts	Rao S, Fujimura T, Matsunari H, Sakuma T, Nakano K, Watanabe M, Asano Y, Kitagawa E, Yamamoto T, Nagashima H Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets. Mol Reprod Dev. 2016 Jan;83(1):61-70.
PAC2 zebrafish fibroblast cells	Sierra S. Nishizaki, Torrin L. McDonald, Gregory A. Farnum, Monica J. Holmes, Melissa L. Drexel, Jessica A. Switzenberg, Alan P. Boyle The inducible lac operator-repressor system is functional in zebrafish cells bioRxiv February 10, 2020
RTgutGC rainbow trout instetine cells	Marina Zoppo, Nicole Okoniewski, Stanislav Pantelyushin, Johannes Vom Berg, Kristin Schirmer A ribonucleoprotein transfection strategy for CRISPR/Cas9-mediated gene editing and single cell cloning in rainbow trout cells Cell Biosci. 2021 Jun 3;11(1):103.

Cell Cultures (adherent electroporation) Primary human epidermal keratinocytes	Watanuki S, Fujita H, Kouyama K, Amagai M, Kubo A Characterization of centriole duplication in human epidermis, Bowen's disease, and squamous cell carcinoma. J Dermatol Sci. 2018 Jul;91(1):9-18.
Retinal Ganglion Cells from human iPSCs	Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells. Sci Rep. 2015 Feb 10;5:8344.
Primary mouse hippocampus and cortex	Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O. Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons. J Neurosci. 2013 Jul 17;33(29):11932-48.
Primary mouse/rat cortical neurons	Masaaki Arai, Osamu Imamura, Nobuo Kondoh, Minori Dateki, Kunio Takishima Neuronal Ca 2+ -dependent activator protein 1 (NCDAP1) induces neuronal cell death by activating p53 pathway following traumatic brain injury J Neurochem. 2019 Dec;151(6):795-809.
Primary mouse Müller glia cells	Hideki Hayashi, Misuzu Mori, Mina Harashima, Tatsuya Hashizume, Miho Furiya, Chihaya Mukaigaito, Emi Takemura, Mariko Yamada, Kanako Mise, Bo Yuan, Norio Takagi Apolipoprotein E-Containing Lipoproteins and LRP1 Protect From NMDA-Induced Excitotoxicity Associated With Reducing α2-Macroglobulin in Müller Glia Invest Ophthalmol Vis Sci. 2021 Oct 4;62(13):23.
Primary mouse myoblasts	Umansky KB, Gruenbaum-Cohen Y, Tsoory M, Feldmesser E, Goldenberg D, Brenner O, Groner Y Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration. PLoS Genet. 2015 Aug 14;11(8):e1005457.
C2C12 myotubes	Perrine Castets, Nathalie Rion, Marine Théodore, Denis Falcetta, Shuo Lin, Markus Reischl, Franziska Wild, Laurent Guérard, Christopher Eickhorst, Marielle Brockhoff, Maitea Guridi, Chikwendu Ibebunjo, Joseph Cruz, Michael Sinnreich, Rüdiger Rudolf, David J Glass, Markus A Rüegg mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4 Nat Commun. 2019 Jul 18;10(1):3187. Cécile P, Julien A, Andrea A, Agnès C, Cécile CG, Clara T, Christiane D, Lydie C, Daniel B, Marco S, Didier A, Daniel T UBE2E1 Is Preferentially Expressed in the Cytoplasm of Slow-Twitch Fibers and Protects Skeletal Muscles from Exacerbated Atrophy upon Dexamethasone Treatment. Cells. 2018 Nov 16;7(11). pii: E214. Gu N, Guo Q, Mao K, Hu H, Jin S, Zhou Y, He H, Oh Y, Liu C, Wu Q Palmitate increases musclin gene expression through activation of PERK signaling pathway in C2C12 myotubes. Biochem Biophys Res Commun. 2015 Nov 20;467(3):521-6. Ohkawara B, Cabrera-Serrano M, Nakata T, Milone M, Asai N, Ito K, Ito M, Masuda A, Ito Y, Engel AG, Ohno K LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet. 2013 Nov 29. [Epub ahead of print]
Primary rat DRG neurons	Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, Hoogenraad CC MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons. Neuron. 2017 Apr 19;94(2):347-362.e7.
COS-7	Katrukha EA, Mikhaylova M, van Brakel HX, van Bergen En Henegouwen PM, Akhmanova A, Hoogenraad CC1, Kapitein LC Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots. Nat Commun. 2017 Mar 21;8:14772.

Plant cells Arabidopsis cells	Furuhata Y1, Sakai A, Murakami T, Morikawa M, Nakamura C, Yoshizumi T, Fujikura U, Nishida K, Kato Y A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall. Sci Rep. 2019 Feb 15;9(1):2163.

Algae Diatom	Weiqi Fu, Amphun Chaiboonchoe, Basel Khraiwesh, Mehar Sultana, Ashish Jaiswal, Kenan Jijakli, David R. Nelson, Ala'a Al-Hrout, Badriya Baig, Amr Amin, and Kourosh Salehi-Ashtiani Intracellular spectral recompositioning of light enhances algal photosynthetic efficiency Sci Adv. 2017 Sep; 3(9): e1603096. Ifuku K, Yan D, Miyahara M, Inoue-Kashino N, Yamamoto YY, Kashino Y A stable and efficient nuclear transformation system for the diatom Chaetoceros gracilis. Photosynth Res. 2015 Feb;123(2):203-11. Miyahara M, Aoi M, Inoue-Kashino N, Kashino Y, Ifuku K. Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation. Biosci Biotechnol Biochem. 2013;77(4):874-6.
Chlamy- domonas	Soujanya Akella, Xinrong Ma, Romana Bacova, Zachary P Harmer, Martina Kolackova, Xiaoxue Wen, David A Wright, Martin H Spalding, Donald P Weeks, Heriberto Cerutti Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas Plant Physiol. 2021 Dec 4;187(4):2637-2655. Nitta N, Sugimura T, Isozaki A, Mikami H, Hiraki K, Sakuma S, Iino T, Arai F, Endo T, Fujiwaki Y, Fukuzawa H, Hase M, Hayakawa T, Hiramatsu K, Hoshino Y, Inaba M, Ito T, Karakawa H, Kasai Y, Koizumi K, Lee S, Lei C, Li M, Maeno T, Matsusaka S, Murakami D, Nakagawa A, Oguchi Y, Oikawa M, Ota T, Shiba K, Shintaku H, Shirasaki Y, Suga K, Suzuki Y, Suzuki N, Tanaka Y, Tezuka H, Toyokawa C, Yalikun Y, Yamada M, Yamagishi M, Yamano T, Yasumoto A, Yatomi Y, Yazawa M, Di Carlo D, Hosokawa Y, Uemura S, Ozeki Y, Goda K Intelligent Image-Activated Cell Sorting. Cell. 2018 Sep 20;175(1):266-276.e13. Nellaepalli S, Ozawa SI, Kuroda H, Takahashi Y The photosystem I assembly apparatus consisting of Ycf3-Y3IP1 and Ycf4 modules. Nat Commun. 2018 Jun 22;9(1):2439. Yamano T, Toyokawa C, Fukuzawa H High-resolution suborganellar localization of Ca2+-binding protein CAS, a novel regulator of CO2-concentrating mechanism. Protoplasma. 2018 Jan 25. [Epub ahead of print] Ishinishi R, Matsuura H, Tanaka S, Nozawa S, Tanada K, Kawashita N, Fujiyama K, Miyasaka H, Hirata K Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga. Gene. 2018 Jan 15;640:14-20. Greiner A, Kelterborn S, Evers H1, Kreimer G, Sizova I, Hegemann P Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-finger Nucleases and CRISPR/Cas9. Plant Cell. 2017 Oct 4. pii: tpc.00659.2017. Nishimura T, Sato F, Ifuku K In vivo system for analyzing the function of the PsbP protein using Chlamydomonas reinhardtii. Photosynth Res. 2017 Sep;133(1-3):117-127. Yamasaki T Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas. Methods Mol Biol. 2017;1640:159-172. Masayuki Onishi and John R. Pringle Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii G3 (Bethesda). 2016 Dec; 6(12): 4115–4125. Wang L, Yamano T, Takane S, Niikawa Y, Toyokawa C, Ozawa SI, Tokutsu R, Takahashi Y, Minagawa J, Kanesaki Y, Yoshikawa H, Fukuzawa H Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12586-12591. Petroutsos D, Tokutsu R, Maruyama S, Flori S, Greiner A, Magneschi L, Cusant L, Kottke T, Mittag M, Hegemann P, Finazzi G, Minagawa J A blue-light photoreceptor mediates the feedback regulation of photosynthesis. Nature. 2016 Sep 22;537(7621):563-566. Onishi M, Pringle JR, Cross FR Evidence That an Unconventional Actin Can Provide Essential F-Actin Function and That a Surveillance System Monitors F-Actin Integrity in Chlamydomonas. Genetics. 2016 Mar;202(3):977-96. Yamano T, Sato E, Iguchi H, Fukuda Y, Fukuzawa H Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7315-20. Wang L, Yamano T, Kajikawa M, Hirono M, Fukuzawa H Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii. Photosynth Res. 2014 Feb 19. [Epub ahead of print] Yamano T, Asada A, Sato E, Fukuzawa H. Isolation and characterization of mutants defective in the localization of LCIB, an essential factor for the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Photosynth Res. 2014 Jan 3. [Epub ahead of print] Yamano T, Iguchi H, Fukuzawa H. Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal. J Biosci Bioeng. 2013 Jun;115(6):691-4.
Euglena	Toshihisa Nomura, Mizuki Yoshikawa, Kengo Suzuki, and Keiichi Mochida Highly Efficient CRISPR-Associated Protein 9 Ribonucleoprotein-Based Genome Editing in Euglena gracilis STAR Protocols. March 27, 2020 Nomura T, Inoue K, Uehara-Yamaguchi Y, Yamada K, Iwata O, Suzuki K, Mochida K Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins. Plant Biotechnol J. 2019 May 26. Mitsuhiro KimuraTakahiro Ishikawa Suppression of DYRK ortholog expression affects wax ester fermentation in Euglena gracilis Journal of Applied Phycology, 2017 Ogawa T, Kimura A, Sakuyama H, Tamoi M, Ishikawa T, Shigeoka S. Identification and characterization of cytosolic fructose-1,6-bisphosphatase in Euglena gracilis. Biosci Biotechnol Biochem. 2015 Jul 27:1-8. Tamaki S, Maruta T, Sawa Y, Shigeoka S, Ishikawa T. Biochemical and physiological analyses of NADPH-dependent thioredoxin reductase isozymes in Euglena gracilis. Plant Sci. 2015 Jul;236:29-36 Ogawa T, Kimura A, Sakuyama H, Tamoi M, Ishikawa T, Shigeoka S. Characterization and physiological role of two types of chloroplastic fructose-1,6-bisphosphatases in Euglena gracilis. Arch Biochem Biophys. 2015 Jun 1;575:61-8. Tamaki S, Maruta T, Sawa Y, Shigeoka S, Ishikawa T. Identification and functional analysis of peroxiredoxin isoforms in Euglena gracilis. Biosci Biotechnol Biochem. 2014;78(4):593-601.
Nannochloropsis	Tomokazu Kurita, Masako Iwai, Keishi Moroi, Kumiko Okazaki, Seiji Nomura, Fumihiko Saito, Shinichiro Maeda, Akihide Takami, Atsushi Sakamoto, Hiroyuki Ohta, Tetsushi Sakuma, Takashi Yamamoto Genome editing with removable TALEN vectors harboring a yeast centromere and autonomous replication sequence in oleaginous microalga Sci Rep. 2022 Feb 15;12(1):2480 Dolch LJ, Rak C, Perin G, Tourcier G, Broughton R, Leterrier M, Morosinotto T, Tellier F, Faure JD, Falconet D, Jouhet J, Sayanova O, Beaudoin F, Maréchal E A Palmitic Acid Elongase Affects Eicosapentaenoic Acid and Plastidial Monogalactosyldiacylglycerol Levels in Nannochloropsis. Plant Physiol. 2017 Jan;173(1):742-759.
Coccomyxa	Yoshimitsu Y, Abe J, Harayama S Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ. Biotechnol Biofuels. 2018 Dec 10;11:326.
Closterium	Junko Kawai, Manaki Kanazawa, Rie Suzuki, Nanako Kikuchi, Yasuhiko Hayakawa, Hiroyuki Sekimoto Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum-strigosum-littorale complex by square-pulse electroporation New Phytol. 2021 Oct 3.
Bodo saltans	Fatma Gomaa, Zhu-Hong Li, David J Beaudoin, Heba Alzan, Peter R Girguis, Roberto Docampo, Virginia P Edgcomb CRISPR/Cas9-induced disruption of Bodo saltans paraflagellar rod-2 gene reveals its importance for cell survival Environ Microbiol. 2022 Jul;24(7):3051-3062.

Yeasts Schizosaccharo- myces pombe	Yuko Takayama Identification of Genes Encoding CENP-A and Heterochromatin Protein 1 of Lipomyces starkeyi and Functional Analysis Using Schizosaccharomyces pombe Genes (Basel). 2020 Jul 8;11(7):E769.

Bacterial cells Staphylococcus aureus	Kotaro Kiga, Xin-Ee Tan, Rodrigo Ibarra-Chávez, Shinya Watanabe, Yoshifumi Aiba, Yusuke Sato'o, Feng-Yu Li, Teppei Sasahara, Bintao Cui, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Aa Haeruman Azam, Masato Suzuki, José R Penadés, Longzhu Cui Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria Nat Commun. 2020 Jun 10;11(1):2934.
E. coli	Tatsuya Yoneji, Hironobu Fujita, Takahito Mukai, Masayuki Su'etsugu Grand scale genome manipulation via chromosome swapping in Escherichia coli programmed by three one megabase chromosomes Nucleic Acids Res. 2021 Apr 28;gkab298. Masahiro Maruoka, Panpan Zhang, Hiromi Mori, Eiichi Imanishi, Daniel M Packwood, Hiroshi Harada, Hidetaka Kosako, Jun Suzuki Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane Mol Cell. 2021 Apr 1;81(7):1397-1410.e9.
Corynebacterim glutamicum	Kyoshiro Nonaka, Tatsuya Osamura, Fumikazu Takahashi A 4-hydroxybenzoate 3-hydroxylase mutant enables 4-amino-3-hydroxybenzoic acid production from glucose in Corynebacterium glutamicum Microb Cell Fact. 2023 Aug 29;22(1):168.

Mouse/Rat Zygotes/ pronuclear- stage embryos (TAKE)	Nanami Kohri, Mitsuo Ota, Hikaru Kousaku, Eiko N. Minakawa, Kazuhiko Seki, Ikuo Tomioka Optimization of piggyBac transposon-mediated gene transfer method in common marmoset embryos PLoS One. 2023 Jun 9;18(6):e0287065. Hend Alhasan, Mohamad Alaa Terkawi, Gen Matsumae, Taku Ebata, Yuan Tian, Tomohiro Shimizu, Yoshio Nishida, Shunichi Yokota, Fayna Garcia-Martin, Mahmoud M Abd Elwakil, Daisuke Takahashi, Mahmoud A Younis, Hideyoshi Harashima, Ken Kadoya, Norimasa Iwasaki Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis Nat Commun. 2022 Jul 7;13(1):3919. Shun Masuda, Shigeru Tanaka, Hiroko Shiraki, Yusuke Sotomaru, Kana Harada, Izumi Hide, Yoshiaki Kiuchi, Norio Sakai GPR3 expression in retinal ganglion cells contributes to neuron survival and accelerates axonal regeneration after optic nerve crush in mice Neurobiol Dis. 2022 Jul 7;172:105811. Matthias Klugmann, Elizabeth Kalotay, Fabien Delerue, Lars M Ittner, Andre Bongers, Josephine Yu, Margaret J Morris, Gary D Housley, Dominik Fröhlich Correction to: Developmental delay and late onset HBSL pathology in hypomorphic Dars1 M256L mice Neurochem Res. 2022 Jul;47(7):1985-1990. Raquel Sanchez-Baltasar, Aida Garcia-Torralba, Virginia Nieto-Romero, Angustias Page, Andrea Molinos-Vicente, Sergio López-Manzaneda, Isabel Ojeda-Pérez, Angel Ramirez, Manuel Navarro, José Carlos Segovia, María García-Bravo Efficient and Fast Generation of Relevant Disease Mouse Models by In Vitro and In Vivo Gene Editing of Zygotes CRISPR J. 2022 Jun;5(3):422-434. Yuki Oyama, Haruhiko Miyata, Keisuke Shimada, Yoshitaka Fujihara, Keizo Tokuhiro, Thomas X Garcia, Martin M Matzuk, Masahito Ikawa CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice Asian J Androl. 2022 May-Jun;24(3):266-272. Shota Tsukimoto, Yoshiyuki Hakata, Sachiyo Tsuji-Kawahara, Takuji Enya, Tetsuo Tsukamoto, Seiya Mizuno, Satoru Takahashi, Shinichi Nakao, Masaaki Miyazawa Distinctive High Expression of Antiretroviral APOBEC3 Protein in Mouse Germinal Center B Cells Viruses. 2022 Apr 17;14(4):832. Jane Chanda Kabwe, Hirofumi Sawada, Yoshihide Mitani, Hironori Oshita, Naoki Tsuboya, Erquan Zhang, Junko Maruyama, Yoshiki Miyasaka, Hideyoshi Ko, Kazunobu Oya, Hiromasa Ito, Noriko Yodoya, Shoichiro Otsuki, Hiroyuki Ohashi, Ryuji Okamoto, Kaoru Dohi, Yuhei Nishimura, Tomoji Mashimo, Masahiro Hirayama, Kazuo Maruyama CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension Respir Res. 2022 Apr 8;23(1):87. Emeline Recazens, Geneviève Tavernier, Jérémy Dufau, Camille Bergoglio, Fadila Benhamed, Stéphanie Cassant-Sourdy, Marie-Adeline Marques, Sylvie Caspar-Bauguil, Alice Brion, Laurent Monbrun, Renaud Dentin, Clara Ferrier, Mélanie Leroux, Pierre-Damien Denechaud, Cedric Moro, Jean-Paul Concordet, Catherine Postic, Etienne Mouisel, Dominique Langin ChREBPβ is dispensable for the control of glucose homeostasis and energy balance JCI Insight. 2022 Feb 22;7(4):e153431. Akihiro Kuno, Yoshihisa Ikeda, Shinya Ayabe, Kanako Kato, Kotaro Sakamoto, Sayaka R Suzuki, Kento Morimoto, Arata Wakimoto, Natsuki Mikami, Miyuki Ishida, Natsumi Iki, Yuko Hamada, Megumi Takemura, Yoko Daitoku, Yoko Tanimoto, Tra Thi Huong Dinh, Kazuya Murata, Michito Hamada, Masafumi Muratani, Atsushi Yoshiki, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes PLoS Biol. 2022 Jan 18;20(1):e3001507. Edgars Liepinsh, Janis Kuka, Karlis Vilks, Baiba Svalbe, Gundega Stelfa, Reinis Vilskersts, Eduards Sevostjanovs, Niks Ricards Goldins, Valerija Groma, Solveiga Grinberga, Mario Plaas, Marina Makrecka-Kuka, Maija Dambrova Low cardiac content of long-chain acylcarnitines in TMLHE knockout mice prevents ischaemia-reperfusion-induced mitochondrial and cardiac damage Free Radic Biol Med. 2021 Dec;177:370-380 Haruki Shinomiya, Hisakazu Kato, Yuki Kuramoto, Nozomi Watanabe, Toshihiro Tsuruda, Tadaaki Arimura, Yohei Miyashita, Yoshiki Miyasaka, Tomoji Mashimo, Ayako Takuwa, Daisuke Motooka, Daisuke Okuzaki, Ken Matsuoka, Osamu Tsukamoto, Hideyuki Hakui, Noriaki Yamada, Jong-Kook Lee, Hidetaka Kioka, Masafumi Kitakaze, Seiji Takashima, Yasushi Sakata, Yoshihiro Asano Aberrant accumulation of TMEM43 accompanied by perturbed transmural gene expression in arrhythmogenic cardiomyopathy FASEB J. 2021 Nov;35(11):e21994. Khursheed Iqbal, Stephen H Pierce, Keisuke Kozai, Pramod Dhakal, Regan L Scott, Katherine F Roby, Carrie A Vyhlidal, Michael J Soares Evaluation of Placentation and the Role of the Aryl Hydrocarbon Receptor Pathway in a Rat Model of Dioxin Exposure Environ Health Perspect. 2021 Nov;129(11):117001. Xiaonan Zhao, Huiyan Lu, Karen Usdin FAN1's protection against CGG repeat expansion requires its nuclease activity and is FANCD2-independent Nucleic Acids Res. 2021 Nov 18;49(20):11643-11652. Akane Morohoshi, Haruhiko Miyata, Yuki Oyama, Seiya Oura, Taichi Noda, Masahito Ikawa FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice Development. 2021 Nov 1;148(21):dev199644. Fabien Thery, Lia Martina, Caroline Asselman, Yifeng Zhang, Madeleine Vessely, Heidi Repo, Koen Sedeyn, George D Moschonas, Clara Bredow, Qi Wen Teo, Jingshu Zhang, Kevin Leandro, Denzel Eggermont, Delphine De Sutter, Katie Boucher, Tino Hochepied, Nele Festjens, Nico Callewaert, Xavier Saelens, Bart Dermaut, Klaus-Peter Knobeloch, Antje Beling, Sumana Sanyal, Lilliana Radoshevich, Sven Eyckerman, Francis Impens Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity Nat Commun. 2021 Oct 1;12(1):5772. Veronika Gresakova, Vendula Novosadova, Michaela Prochazkova, Jan Prochazka, Radislav Sedlacek Dual role of Fam208a during zygotic cleavage and early embryonic development Exp Cell Res. 2021 Sep 15;406(2):112723. Shigenobu Matsumura, Fuka Ishikawa, Tsutomu Sasaki, Mike Krogh Terkelsen, Kim Ravnskjaer, Tomoki Jinno, Jin Tanaka, Tsuyoshi Goto, Kazuo Inoue Loss of CREB Coactivator CRTC1 in SF1 Cells Leads to Hyperphagia and Obesity by High-fat Diet But Not Normal Chow Diet Endocrinology. 2021 Sep 1;162(9):bqab076. Hirofumi Fujita, Munenori Habuta, Takako Hattori, Satoshi Kubota, Hiromi Kumon, Hideyo Ohuchi UCP1 expression in the mouse adrenal gland is not upregulated by thermogenic conditions Biochem Biophys Res Commun. 2021 Aug 20;566:184-189. Yasuhiro Yamamoto, Haruka Chino, Satoshi Tsukamoto, Koji L Ode, Hiroki R Ueda, Noboru Mizushima NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA Nat Commun. 2021 Jun 2;12(1):3292. Megumi Ibayashi, Ryutaro Aizawa, Satoshi Tsukamoto mRNA decapping factor Dcp1a is essential for embryonic growth in mice Biochem Biophys Res Commun. 2021 May 28;555:128-133. Kazushi Aoto, Mitsuhiro Kato, Tenpei Akita, Mitsuko Nakashima, Hiroki Mutoh, Noriyuki Akasaka, Jun Tohyama, Yoshiko Nomura, Kyoko Hoshino, Yasuhiko Ago, Ryuta Tanaka, Orna Epstein, Revital Ben-Haim, Eli Heyman, Takehiro Miyazaki, Hazrat Belal, Shuji Takabayashi, Chihiro Ohba, Atsushi Takata, Takeshi Mizuguchi, Satoko Miyatake, Noriko Miyake, Atsuo Fukuda, Naomichi Matsumoto, Hirotomo Saitsu ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H +-ATPases is essential for brain development in humans and mice Nat Commun. 2021 Apr 8;12(1):2107. Ji Min Lee, Ukjin Kim, Hyokyung Yang, Bokyeong Ryu, Jin Kim, Tetsushi Sakuma, Takashi Yamamoto, Jae-Hak Park TALEN-mediated generation of Nkx3.1 knockout rat model Prostate. 2021 Feb;81(3):182-193. Nan-Ee Lee, Dae-In Ha, Jeong-Heon Ko, Yong-Sam Kim One-step genotyping method in CRISPR based on short inner primer-assisted, tetra primer-paired amplifications Mol Cell Probes. 2021 Feb;55:101675. Sandrine Vandormael-Pournin, Emmanuel Frachon, Samy Gobaa, Michel Cohen-Tannoudji Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos Methods Mol Biol. 2021;2214:11-30. Irena Jenickova, Petr Kasparek, Silvia Petrezselyova, Jan Elias, Jan Prochazka, Jana Kopkanova, Michal Navratil, Cyril Barinka, Radislav Sedlacek Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein Methods. 2020 Jul 24;S1046-2023(20)30114-6. Yui Wake, Takehito Kaneko Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation J Reprod Dev. 2020 Jul 24. Online ahead of print. Kento Morimoto, Koki Numata, Yoko Daitoku, Yuko Hamada, Keiko Kobayashi, Kanako Kato, Hayate Suzuki, Shinya Ayabe, Atsushi Yoshiki, Satoru Takahashi, Kazuya Murata, Seiya Mizuno, Fumihiro Sugiyama Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis Sci Rep. 2020 Jun 3;10(1):9060. Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel, Chaowan Guo, Yasukazu Daigaku, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato Mitsutake, Takayoshi Suganami, Akio Masuda, Kinji Ohno, Shinichiro Nakada, Tomoji Mashimo, Koji Yamanaka, Martijn S Luijsterburg, Tomoo Ogi Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair Cell. 2020 Mar 19;180(6):1228-1244.e24. Toshihiro Kobayashi, Hisato Kobayashi, Teppei Goto, Tomoya Takashima, Mami Oikawa, Hiroki Ikeda, Reiko Terada, Fumika Yoshida, Makoto Sanbo, Hiromitsu Nakauchi, Kazuki Kurimoto, Masumi Hirabayashi Germline Development in Rat Revealed by Visualization and Deletion of Prdm14 Development, 147 (4) 2020 Feb 21 Takuya Takeichi, Tetsuya Hirabayashi, Yuki Miyasaka, Akane Kawamoto, Yusuke Okuno, Shijima Taguchi, Kana Tanahashi, Chiaki Murase, Hiroyuki Takama, Kosei Tanaka, William E Boeglin, M Wade Calcutt, Daisuke Watanabe, Michihiro Kono, Yoshinao Muro, Junko Ishikawa, Tamio Ohno, Alan R Brash, Masashi Akiyama SDR9C7 Catalyzes Critical Dehydrogenation of Acylceramides for Skin Barrier Formation J Clin Invest, 130 (2), 890-903 2020 Feb 3 Hiroyuki Kondo, Takafumi Matsumura, Mari Kaneko, Kenichi Inoue, Hidetaka Kosako, Masahito Ikawa, Yousuke Takahama, Izumi Ohigashi PITHD1 Is a Proteasome-Interacting Protein Essential for Male Fertilization J Biol Chem, 295 (6), 1658-1672 2020 Feb 7 Yeon Sun Kim, Gyeong Ryeong Kim, Mira Park, Seung Chel Yang, So Hee Park, Ji Eun Won, Ju Hee Lee, Ha Eun Shin, Haengseok Song, Hye-Ryun Kim Electroporation of AsCpf1/RNP at the Zygote Stage Is an Efficient Genome Editing Method to Generate Knock-Out Mice Deficient in Leukemia Inhibitory Factor Tissue Eng Regen Med, 17 (1), 45-53 Feb 2020 Takehito Kaneko, Yuki Nakagawa Genome Editing of Rodents by Electroporation of CRISPR/Cas9 Into Frozen-Warmed Pronuclear-Stage Embryos Cryobiology 2020 Jan 24 [Online ahead of print] Kumiko Yoshioka-Kobayashi, Marina Matsumiya, Yusuke Niino, Akihiro Isomura, Hiroshi Kori, Atsushi Miyawaki, Ryoichiro Kageyama Coupling Delay Controls Synchronized Oscillation in the Segmentation Clock Nature 2020 Jan 8[Online ahead of print] Tomoyuki Tsukiyama, Kenichi Kobayashi, Masataka Nakaya, Chizuru Iwatani, Yasunari Seita, Hideaki Tsuchiya, Jun Matsushita, Kahoru Kitajima, Ikuo Kawamoto, Takahiro Nakagawa, Koji Fukuda, Teppei Iwakiri, Hiroyuki Izumi, Iori Itagaki, Shinji Kume, Hiroshi Maegawa, Ryuichi Nishinakamura, Saori Nishio, Shinichiro Nakamura, Akihiro Kawauchi, Masatsugu Ema Monkeys Mutant for PKD1 Recapitulate Human Autosomal Dominant Polycystic Kidney Disease Nat Commun, 10 (1), 5517 2019 Dec 11 Charles-Etienne Dumeau, Asun Monfort, Lucas Kissling, Daan C Swarts, Martin Jinek, Anton Wutz Introducing Gene Deletions by Mouse Zygote Electroporation of Cas12a/Cpf1 Transgenic Res, 28 (5-6), 525-535 Dec 2019 Shiro Yamashita, Yuhei Kogasaka, Yuuki Hiradate, Kentaro Tanemura, Yutaka Sendai Suppression of Mosaic Mutation by Co-Delivery of CRISPR Associated Protein 9 and Three-Prime Repair Exonuclease 2 Into Porcine Zygotes via Electroporation J Reprod Dev, 66 (1), 41-48 2020 Feb 14 Peiheng Gan, Michaela Patterson, Alexa Velasquez, Kristy Wang, Di Tian, Jolene J Windle, Ge Tao, Daniel P Judge, Takako Makita, Thomas J Park, Henry M Sucov Tnni3k Alleles Influence Ventricular Mononuclear Diploid Cardiomyocyte Frequency PLoS Genet, 15 (10), e1008354 2019 Oct 7 eCollection Oct 2019 Iwata S, Nakadai H, Fukushi D, Jose M, Nagahara M, Iwamoto T Simple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation. Sci Rep. 2019 Oct 11;9(1):14713. Nakagawa Y, Kaneko T Rapid and efficient production of genome-edited animals by electroporation into oocytes injected with frozen or freeze-dried sperm. Cryobiology. 2019 Aug 22. pii: S0011-2240(19)30215-9. Honda A, Tachibana R, Hamada K, Morita K, Mizuno N, Morita K, Asano M Efficient derivation of knock-out and knock-in rats using embryos obtained by in vitro fertilization. Sci Rep. 2019 Aug 9;9(1):11571. Yonggang Lu, Seiya Oura, Takafumi Matsumura, Asami Oji, Nobuyuki Sakurai, Yoshitaka Fujihara, Keisuke Shimada, Haruhiko Miyata, Tomohiro Tobita, Taichi Noda, Julio M Castaneda, Daiji Kiyozumi, Qian Zhang, Tamara Larasati, Samantha A M Young, Mayo Kodani, Caitlin A Huddleston, Matthew J Robertson, Cristian Coarfa, Ayako Isotani, R John Aitken, Masaru Okabe, Martin M Matzuk, Thomas X Garcia, Masahito Ikawa CRISPR/Cas9-mediated Genome Editing Reveals 30 Testis-Enriched Genes Dispensable for Male Fertility in Mice Biol Reprod, 101 (2), 501-511 2019 Aug 1 Jinxi Wang, Ruihua Dang, Yoshiki Miyasaka, Kousuke Hattori, Daisuke Torigoe, Tadashi Okamura, Hassan T Tag-Ei-Din-Hassan, Masami Morimatsu, Tomoji Mashimo, Takashi Agui Null Mutation of the Endothelin Receptor Type B Gene Causes Embryonic Death in the GK Rat PLoS One, 14 (6), e0217132 2019 Jun 6 eCollection 2019 T Noda, N Sakurai, K Nozawa, S Kobayashi, D J Devlin, M M Matzuk, M Ikawa Nine Genes Abundantly Expressed in the Epididymis Are Not Essential for Male Fecundity in Mice Andrology, 7 (5), 644-653 Sep 2019 Ayabe S, Nakashima K, Yoshiki A Off- and on-target effects of genome editing in mouse embryos. J Reprod Dev. 2019 Feb 8;65(1):1-5. Sho Yoshimatsu, Junko Okahara, Takefumi Sone, Yuta Takeda, Mari Nakamura, Erika Sasaki, Noriyuki Kishi, Seiji Shiozawa, Hideyuki Okano Robust and Efficient Knock-In in Embryonic Stem Cells and Early-Stage Embryos of the Common Marmoset Using the CRISPR-Cas9 System Sci Rep, 9 (1), 1528 2019 Feb 6 Abbasi F, Miyata H1, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, Ikawa M RSPH6A is required for sperm flagellum formation and male fertility in mice. J Cell Sci. 2018 Oct 11;131(19). Kaneko T Reproductive technologies for the generation and maintenance of valuable animal strains. J Reprod Dev. 2018 Jun 22;64(3):209-215. Miyasaka Y, Uno Y, Yoshimi K, Kunihiro Y, Yoshimura T, Tanaka T, Ishikubo H, Hiraoka Y, Takemoto N, Tanaka T, Ooguchi Y, Skehel P, Aida T, Takeda J, Mashimo T CLICK: one-step generation of conditional knockout mice. BMC Genomics. 2018 May 2;19(1):318. Teixeira M, Py BF, Bosc C, Laubreton D, Moutin MJ, Marvel J, Flamant F, Markossian S Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing. Sci Rep. 2018 Jan 11;8(1):474. Remy S, Chenouard V, Tesson L, Usal C, Ménoret S, Brusselle L, Heslan JM, Nguyen TH, Bellien J, Merot J, De Cian A, Giovannangeli C, Concordet JP, Anegon IS Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation. Sci Rep. 2017 Nov 29;7(1):16554. Kim K, Ryu SM, Kim ST, Baek G, Kim D, Lim K, Chung E, Kim S, Kim JS Highly efficient RNA-guided base editing in mouse embryos. Nat Biotechnol. 2017 Feb 27. [Epub ahead of print] Takehito Kaneko Genome Editing in Mouse and Rat by Electroporation Methods Mol Biol, 1630, 81-89 2017 Hur JK, Kim K, Been KW, Baek G, Ye S, Hur JW, Ryu SM, Lee YS, Kim JS Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins. Nat Biotechnol. 2016 Aug;34(8):807-8. Kaneko T, Mashimo T Simple Genome Editing of Rodent Intact Embryos by Electroporation. PLoS One. 2015 Nov 10;10(11):e0142755 Kaneko T, Sakuma T, Yamamoto T, Mashimo T Simple knockout by electroporation of engineered endonucleases into intact rat embryos. Sci Rep. 2014 Oct 1;4:6382.

Mouse/Rat Embryos in oviduct (i-GONAD)	Shun-Suke Sakai, Atsushi Hasegawa, Ryosuke Ishimura, Naoki Tamura, Shun Kageyama, Satoko Komatsu-Hirota, Manabu Abe, Yiwei Ling, Shujiro Okuda, Manabu Funayama, Mika Kikkawa, Yoshiki Miura, Kenji Sakimura, Ichiei Narita, Satoshi Waguri, Ritsuko Shimizu, Masaaki Komatsu Loss of Atg2b and Gskip Impairs the Maintenance of the Hematopoietic Stem Cell Pool Size Mol Cell Biol. 2022 Jan 20;42(1):e0002421. Masumi Namba, Tomoe Kobayashi, Takayuki Koyano, Mayumi Kohno, Masato Ohtsuka, Makoto Matsuyama GONAD: A new method for germline genome editing in mice and rats Dev Growth Differ. 2021 Aug 25. Satoru Iwata, Takahisa Sasaki, Miki Nagahara, Takashi Iwamoto An efficient i-GONAD method for creating and maintaining lethal mutant mice using an inversion balancer identified from the C3H/HeJJcl strain G3 Genes\|Genomes\|Genetics, jkab194, 03 June 2021 Masumi Namba, Tomoe Kobayashi, Mayumi Kohno, Takayuki Koyano, Takuo Hirose, Masaki Fukushima, Makoto Matsuyama Creation of X-linked Alport Syndrome Rat Model with Col4a5 Deficiency bioRxiv, April 14, 2021 Haruo Nakano, Shiori Kawai, Yusaku Ooki, Tomoki Chiba, Chiharu Ishii, Takumi Nozawa, Hisako Utsuki, Mariko Umemura, Shigeru Takahashi, Yuji Takahashi Functional validation of epitope-tagged ATF5 knock-in mice generated by improved genome editing of oviductal nucleic acid delivery (i-GONAD) Cell Tissue Res. 2021 Apr 7. Online ahead of print. Takehito Kaneko, Shungo Tanaka Improvement of genome editing by electroporation using embryos artificially removed cumulus cells in the oviducts Biochem Biophys Res Commun. 2020 Jul 5;527(4):1039-1042. Masahiro Sato, Rico Miyagasako, Shuji Takabayashi, Masato Ohtsuka, Izuho Hatada, Takuro Horii Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice Cells, 9 (3) 2020 Feb 26 Iwata S, Nakadai H, Fukushi D, Jose M, Nagahara M, Iwamoto T Simple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation. Sci Rep. 2019 Oct 11;9(1):14713. Takayuki Koyano, Masumi Namba, Tomoe Kobayashi, Kyomi Nakakuni, Daisuke Nakano, Masaki Fukushima, Akira Nishiyama, Makoto Matsuyama The p21 dependent G2 arrest of the cell cycle in epithelial tubular cells links to the early stage of renal fibrosis. Sci Rep. 2019 Aug 19;9:12059. Takabayashi S, Aoshima T, Kabashima K, Aoto K, Ohtsuka M, Sato M i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats. Sci Rep. 2018 Aug 13;8(1):12059. Kobayashi T, Namba M, Koyano T, Fukushima M, Sato M, Ohtsuka M, Matsuyama M Successful production of genome-edited rats by the rGONAD method. BMC Biotechnol. 2018 Apr 2;18(1):19

Mouse/Rat Embryos in utero	Marcello Scala, Masashi Nishikawa, Hidenori Ito, Hidenori Tabata, Tayyaba Khan, Andrea Accogli, Laura Davids, Anna Ruiz, Pietro Chiurazzi, Gabriella Cericola, Björn Schulte, Kristin G Monaghan, Amber Begtrup, Annalaura Torella, Michele Pinelli, Anne Sophie Denommé-Pichon, Antonio Vitobello, Caroline Racine, Maria Margherita Mancardi, Courtney Kiss, Andrea Guerin, Wendy Wu, Elisabeth Gabau Vila, Bryan C Mak, Julian A Martinez-Agosto, Michael B Gorin, Bugrahan Duz, Yavuz Bayram, Claudia M B Carvalho, Jaime E Vengoechea, David Chitayat, Tiong Yang Tan, Bert Callewaert, Bernd Kruse, Lynne M Bird, Laurence Faivre, Marcella Zollino, Saskia Biskup, Pasquale Striano, Vincenzo Nigro, Mariasavina Severino, Valeria Capra, Gregory Costain, Koh Ichi Nagata Variant-specific changes in RAC3 function disrupt corticogenesis in neurodevelopmental phenotypes Brain. 2022 Sep 14;145(9):3308-3327. Inmaculada Crespo, Jaime Pignatelli, Veena Kinare, Héctor R Méndez-Gómez, Miriam Esgleas, María José Román, Josep M Canals, Shubha Tole, Carlos Vicario Tbr1 Misexpression Alters Neuronal Development in the Cerebral Cortex Mol Neurobiol. 2022 Sep;59(9):5750-5765. Tamar Sapir, Aditya Kshirsagar, Anna Gorelik, Tsviya Olender, Ziv Porat, Ingrid E Scheffer, David B Goldstein, Orrin Devinsky, Orly Reiner Heterogeneous nuclear ribonucleoprotein U (HNRNPU) safeguards the developing mouse cortex Nat Commun. 2022 Jul 21;13(1):4209. Masashi Nishikawa, Hidenori Ito, Hidenori Tabata, Hiroshi Ueda, Koh-Ichi Nagata Impaired Function of PLEKHG2, a Rho-Guanine Nucleotide-Exchange Factor, Disrupts Corticogenesis in Neurodevelopmental Phenotypes Cells. 2022 Feb 16;11(4):696. Yuki Bando, Michael Wenzel, Rafael Yuste Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo Nat Commun. 2021 Dec 10;12(1):7229. Yasufumi Hayano, Yugo Ishino, Jung Ho Hyun, Carlos G Orozco, André Steinecke, Elizabeth Potts, Yasuhiro Oisi, Connon I Thomas, Debbie Guerrero-Given, Eunjoon Kim, Hyung-Bae Kwon, Naomi Kamasawa, Hiroki Taniguchi IgSF11 homophilic adhesion proteins promote layer-specific synaptic assembly of the cortical interneuron subtype Sci Adv. 2021 Jul 14;7(29):eabf1600. Mina Takechi, Kenzi Oshima, Daita Nadano, Hiroshi Kitagawa, Tsukasa Matsuda, Shinji Miyata A pericellular hyaluronan matrix is required for the morphological maturation of cortical neurons Biochim Biophys Acta Gen Subj. 2020 Oct;1864(10):129679. Monia Barnat, Mariacristina Capizzi, Esther Aparicio, Susana Boluda, Doris Wennagel, Radhia Kacher, Rayane Kassem, Sophie Lenoir, Fabienne Agasse, Barbara Y Braz, Jeh-Ping Liu, Julien Ighil, Aude Tessier, Scott O Zeitlin, Charles Duyckaerts, Marc Dommergues, Alexandra Durr, Sandrine Humbert Huntington's disease alters human neurodevelopment Science. 2020 Aug 14;369(6505):787-793. Masashi Ueda, Tohru Matsuki, Masahide Fukada, Shima Eda, Akie Toya, Akio Iio, Hidenori Tabata, Atsuo Nakayama Knockdown of Son, a mouse homologue of the ZTTK syndrome gene, causes neuronal migration defects and dendritic spine abnormalities Mol Brain. 2020 May 24;13(1):80. Naohiro Kuwayama, Yusuke Kishi, Yurie Maeda, Yurie Nishiumi, Yutaka Suzuki, Haruhiko Koseki, Yusuke Hirabayashi, Yukiko Gotoh In utero gene transfer system for embryos before neural tube closure reveals a role for Hmga2 in the onset of neurogenesis bioRxiv May 15, 2020 Taiga Kurihara, Emi Kouyama-Suzuki, Michiru Satoga, Xue Li, Moataz Badawi, Thiha, Deeba Noreen Baig, Toru Yanagawa, Takeshi Uemura, Takuma Mori, Katsuhiko Tabuchi DNA Repair Protein RAD51 Enhances the CRISPR/Cas9-mediated Knock-In Efficiency in Brain Neurons Biochem Biophys Res Commun. 2020 Apr 9;524(3):621-628. Tal Laviv, Benjamin Scholl, Paula Parra-Bueno, Beth Foote, Chuqiu Zhang, Long Yan, Yuki Hayano, Jun Chu, Ryohei Yasuda In Vivo Imaging of the Coupling Between Neuronal and CREB Activity in the Mouse Brain Neuron, 105 (5), 799-812.e5 2020 Mar 4 Julie Stoufflet, Maxime Chaulet, Mohamed Doulazmi, Coralie Fouquet, Caroline Dubacq, Christine Métin, Alain Trembleau, Pierre Vincent, Isabelle Caillé Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration bioRxiv November 12, 2019 Hiroki Takeda, Toru Miwa, Min Young Kim, Byung Yoon Choi, Yorihisa Orita, Ryosei Minoda Prenatal Electroporation-Mediated Gene Transfer Restores Slc26a4 Knock-Out Mouse Hearing and Vestibular Function Sci Rep, 9 (1), 17979 2019 Nov 29 Feng Liu, Chao Du, Xin Tian, Yuanlin Ma, Bei Zhao, Yin Yan, Zijun Lin, Peijia Lin, Ruijiao Zhou, Xuefeng Wang A Novel LGI1 Missense Mutation Causes Dysfunction in Cortical Neuronal Migration and Seizures Brain Res, 1721, 146332 2019 Oct 15 Toru Miwa, Ryosei Minoda, Yoshihide Ishikawa, Tomohito Kajii, Yorihisa Orita, Takahiro Ohyama Role of Dach1 Revealed Using a Novel Inner Ear-Specific Dach1-knockdown Mouse Model Biol Open, 8 (8) 2019 Aug 20 André Steinecke, Nobuhiro Kurabayashi, Yasufumi Hayano, Yugo Ishino, Hiroki Taniguchi In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected With CRISPR/Cas9 Cell Rep, 28 (2), 325-331.e4 2019 Jul 9 Minkyung Shin, Ayako Kitazawa, Satoshi Yoshinaga, Kanehiro Hayashi, Yukio Hirata, Colette Dehay, Ken-Ichiro Kubo, Kazunori Nakajima Both Excitatory and Inhibitory Neurons Transiently Form Clusters at the Outermost Region of the Developing Mammalian Cerebral Neocortex J Comp Neurol, 527 (10), 1577-1597 2019 Jul 1 Yu-Jie Lai, Bing-Lin Zhu, Fei Sun, Dong Luo, Yuan-Lin Ma, Bio Luo, Jing Tang, Ming-Jian Xiong, Lu Liu, Yan Long, Xiao-Tong Hu, Ling He, Xiao-Juan Deng, John H Zhang, Jian Yang, Zhen Yan, Guo-Jun Chen Estrogen Receptor α Promotes Cav1.2 Ubiquitination and Degradation in Neuronal Cells and in APP/PS1 Mice Aging Cell, 18 (4), e12961 Aug 2019 Oriol Ros, Yvrick Zagar, Solène Ribes, Sarah Baudet, Karine Loulier, Sandrine Couvet, Delphine Ladarre, Alain Aghaie, Alice Louail, Christine Petit, Yves Mechulam, Zsolt Lenkei, Xavier Nicol SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation Cell Rep, 27 (13), 4003-4012.e6 2019 Jun 25 Margarita Arango-Lievano, Amelie M Borie, Yann Dromard, Maxime Murat, Michel G Desarmenien, Michael J Garabedian, Freddy Jeanneteau Persistence of Learning-Induced Synapses Depends on Neurotrophic Priming of Glucocorticoid Receptors Proc Natl Acad Sci U S A, 116 (26), 13097-13106 2019 Jun 25 Mariko Noda, Ikuko Iwamoto, Hidenori Tabata, Takanori Yamagata, Hidenori Ito, Koh-Ichi Nagata Role of Per3, a Circadian Clock Gene, in Embryonic Development of Mouse Cerebral Cortex Sci Rep, 9 (1), 5874 2019 Apr 10 Isis Trujillo-Gonzalez, Yanyan Wang, Walter B Friday, Kasey C Vickers, Cynthia L Toth, Lorian Molina-Torres, Natalia Surzenko, Steven H Zeisel MicroRNA-129-5p Is Regulated by Choline Availability and Controls EGF Receptor Synthesis and Neurogenesis in the Cerebral Cortex FASEB J, 33 (3), 3601-3612 Mar 2019 Michael J Yetman, Eric Washburn, Jung Ho Hyun, Fumitaka Osakada, Yasufumi Hayano, Hongkui Zeng, Edward M Callaway, Hyung-Bae Kwon, Hiroki Taniguchi Intersectional Monosynaptic Tracing for Dissecting Subtype-Specific Organization of GABAergic Interneuron Inputs Nat Neurosci, 22 (3), 492-502 Mar 2019 Bartosz Tarkowski, Kinga Kuchcinska, Magdalena Blazejczyk, Jacek Jaworski Pathological mTOR Mutations Impact Cortical Development Hum Mol Genet, 28 (13), 2107-2119 2019 Jul 1 N. A. Simonova, N. V. Bal, P. M. Balaban, M. A. Volgushev, A. Y. Malyshev An Optogenetic Approach to Studies of the Mechanisms of Heterosynaptic Plasticity in Neocortical Neurons Neuroscience and Behavioral Physiology volume 49, pages208–215(2019) Seika Inoue, Kanehiro Hayashi, Kyota Fujita, Kazuhiko Tagawa, Hitoshi Okazawa, Ken-Ichiro Kubo, Kazunori Nakajima Drebrin-like (Dbnl) Controls Neuronal Migration via Regulating N-Cadherin Expression in the Developing Cerebral Cortex J Neurosci, 39 (4), 678-691 2019 Jan 23 Yuki Bando, Masayuki Sakamoto, Samuel Kim, Inbal Ayzenshtat, Rafael Yuste Comparative Evaluation of Genetically Encoded Voltage Indicators Cell Rep, 26 (3), 802-813.e4 2019 Jan 15 Dawei Zhang, Shanzheng Yang, Enrique M Toledo, Daniel Gyllborg, Carmen Saltó, J Carlos Villaescusa, Ernest Arenas Niche-derived laminin-511 Promotes Midbrain Dopaminergic Neuron Survival and Differentiation Through YAP Sci Signal, 10 (493) 2017 Aug 22 Anna Gorelik, Tamar Sapir, Rebecca Haffner-Krausz, Tsviya Olender, Trent M Woodruff, Orly Reiner Developmental Activities of the Complement Pathway in Migrating Neurons Nat Commun, 8, 15096 2017 May 2 Lecuyer M, Laquerrière A, Bekri S, Lesueur C, Ramdani Y, Jégou S, Uguen A, Marcorelles P, Marret S, Gonzalez BJ PLGF, a placental marker of fetal brain defects after in utero alcohol exposure. Acta Neuropathol Commun. 2017 Jun 6;5(1):44. Monia Barnat, Julien Le Friec, Caroline Benstaali, Sandrine Humbert Huntingtin-Mediated Multipolar-Bipolar Transition of Newborn Cortical Neurons Is Critical for Their Postnatal Neuronal Morphology Neuron. 2017 Jan 4;93(1):99-114. Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, Jafari M, Esteban CR, Berggren WT, Lajara J, Nuñez-Delicado E, Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway EM, Zhang K, Belmonte JC In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature. 2016 Dec 1;540(7631):144-149 Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, Brandon NJ, Maher BJ, Kamiya A Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1. Mol Psychiatry. 2016 Oct;21(10):1449-59. Yuji Tsunekawa, Raymond Kunikane Terhune, Ikumi Fujita, Atsunori Shitamukai, Taeko Suetsugu, Fumio Matsuzaki Developing a De Novo Targeted Knock-In Method Based on in Utero Electroporation Into the Mammalian Brain Development, 143 (17), 3216-22 2016 Sep 1 Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Nat Biotechnol. 2016 Aug 29 /nbt.3658. [Epub ahead of print] Mikuni T, Nishiyama J, Sun Y, Kamasawa N, Yasuda R High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Cell. 2016 Jun 16;165(7):1803-17. Szczurkowska J, Cwetsch AW, dal Maschio M, Ghezzi D, Ratto GM, Cancedda L Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe. Nat Protoc. 2016 Mar;11(3):399-412. Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, Brandon NJ, Maher BJ, Kamiya A Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1. Mol Psychiatry. 2016 Jan 5. Christoph Straub, Adam J Granger, Jessica L Saulnier, Bernardo L Sabatini CRISPR/Cas9-mediated Gene Knock-Down in Post-Mitotic Neurons PLoS One, 9 (8), e105584 2014 Aug 20 eCollection 2014 Yumoto T, Nakadate K, Nakamura Y, Sugitani Y, Sugitani-Yoshida R, Ueda S, Sakakibara S. Radmis, a Novel Mitotic Spindle Protein that Functions in Cell Division of Neural Progenitors. PLoS One. 2013 Nov 8;8(11):e79895. Kita Y, Kawakami K, Takahashi Y, Murakami F. Development of cerebellar neurons and glias revealed by in utero electroporation: Golgi-like labeling of cerebellar neurons and glias. PLoS One. 2013 Jul 23;8(7):e70091. Sapir T, Levy T, Sakakibara A, Rabinkov A, Miyata T, Reiner O. Shootin1 Acts in Concert with KIF20B to Promote Polarization of Migrating Neurons. J Neurosci. 2013 Jul 17;33(29):11932-48. Tabata H, Hachiya T, Nagata K, Sakakibara Y, Nakajima K. Screening for candidate genes involved in the production of mouse subventricular zone proliferative cells and an estimation of their changes in evolutionary pressure during primate evolution. Front Neuroanat. 2013 Jul 31;7:24. Nishiyama J, Hayashi Y, Nomura T, Miura E, Kakegawa W, Yuzaki M. Selective and regulated gene expression in murine Purkinje cells by in utero electroporation. Eur J Neurosci. 2012 Oct;36(7):2867-76. Miyata S, Komatsu Y, Yoshimura Y, Taya C, Kitagawa H. Persistent cortical plasticity by upregulation of chondroitin 6-sulfation. Nat Neurosci. 2012 Jan 15;15(3):414-22, S1-2. Niwa M, Kamiya A, Murai R, Kubo K, Gruber AJ, Tomita K, Lu L, Tomisato S, Jaaro-Peled H, Seshadri S, Hiyama H, Huang B, Kohda K, Noda Y, O'Donnell P, Nakajima K, Sawa A, Nabeshima T. Knockdown of DISC1 by in utero gene transfer disturbs postnatal dopaminergic maturation in the frontal cortex and leads to adult behavioral deficits. Neuron. 2010 Feb 25;65(4):480-9. Akemann W, Mutoh H, Perron A, Rossier J, Knöpfel T. Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins Nature Methods, Volume 7, Number8 Pages 643-649, August 2010 Zhu LQ, Zheng HY, Peng CX, Liu D, Li HL, Wang Q, Wang JZ. Protein phosphatase 2A facilitates axonogenesis by dephosphorylating CRMP2. J Neurosci. 2010 Mar 10;30(10):3839-48. Miyashita T, Wintzer M, Kurotani T, Konishi T, Ichinohe N, Rockland KS. Neurotrophin-3 is involved in the formation of apical dendritic bundles in cortical layer 2 of the rat. Cereb Cortex. 2010 Jan;20(1):229-40. *Rat embryos Cina C, Maass K, Theis M, Willecke K, Bechberger JF, Naus CC. Involvement of the cytoplasmic C-terminal domain of connexin43 in neuronal migration. J Neurosci. 2009 Feb 18;29(7):2009-21. Brigande JV, Gubbels SP, Woessner DW, Jungwirth JJ, Bresee CS. Electroporation-mediated gene transfer to the developing mouse inner ear. Methods Mol Biol. 2009;493:125-39. Tabata H, Nakajima K. Labeling embryonic mouse central nervous system cells by in utero electroporation. Dev Growth Differ. 2008 Aug;50(6):507-11. Prozorovski T, Schulze-Topphoff U, Glumm R, Baumgart J, Schröter F, Ninnemann O, Siegert E, Bendix I, Brüstle O, Nitsch R, Zipp F, Aktas O. Sirt1 contributes critically to the redox-dependent fate of neural progenitors Nature Cell Biology, Volume 10, Number 4, Pages 385-394, April 2008 Muraguchi T, Takegami Y, Ohtsuka T, Kitajima S, Chandana EP, Omura A, Miki T, Takahashi R, Matsumoto N, Ludwig A, Noda M, Takahashi C. RECK modulates Notch signaling during cortical neurogenesis by regulating ADAM10 activity Nature Neuroscience, Volume 10, Number 7, Pages 838-845, July 2007 Nakahira E, Kagawa T, Shimizu T, Goulding MD, Ikenaka K Direct evidence that ventral forebrain cells migrate to the cortex and contribute to the generation of cortical myelinating oligodendrocytes. Dev Biol. 2006 Mar 1;291(1):123-31. Takeuchi A, O'Leary DD. Radial Migration of Superficial Layer Cortical Neurons Controlled by Novel Ig Cell Adhesion Molecule MDGA1 The Journal of Neuroscience, Volume 26, Issue 17, Pages 4460-4464, 26 April 2006 Saito T. In vivo electroporation in the embryonic mouse central nervous system Nature Protocols, Volume 1, Number 3, Pages 1552-1558, 2006 Saba R1, Johnson JE, Saito T. Commissural neuron identity is specified by a homeodomain protein, Mbh1, that is directly downstream of Math1 Development, Volume 132, Issue 9, Pages 2147-2155, May 2005 Eiko Nakahira and Shigeki Yuasa Neuronal generation, migration, and differentiation in the mouse hippocampal primoridium as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation The Journal of Comparative Neurology, Volume 483, Issue 3, Pages 329-340, 14 March 2005 Kamon Sanada and Li-Huei Tsai G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors Cell, Volume 122, Issue 1, Pages 119-131, 15 July 2005 Itsuki Ajioka and Kazunori Nakajima Birth-date-dependent segregation of the mouse cerebral cortical neurons in reaggregation cultures European Journal of Neuroscience, Volume 22, Issue 2, Pages 331-342, July 2005 Takiguchi-Hayashi et al. Generation of Reelin-Positive Marginal Zone Cells from the Caudomedial Wall of Telencephalic Vesicles The Journal of Neuroscience, Volume 24, Issue 9, Pages 2286-2295, 3 March 2004 Nguyen et al. A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons Nature Cell Biology, Volume 6, Number 7, Pages 595-608, July 2004 Hatakeyama et al. Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation Development, Volume 131, Issue 22, Pages 5539-5550, November 2004 Saba et al. Mammalian BarH1 Confers Commissural Neuron Identity on Dorsal Cells in the Spinal Cord The Journal of Neuroscience, Volume 23, Issue 6, Pages 1987-1991, 15 March 2003 Kawauchi et al. The in vivo roles of STEF/Tiam1, Rac1 and JNK in cortical neuronal migration The EMBO Journal, Volume 22, Number 16, Pages 4190-4201, August 2003 Gupta et al. Layering defect in p35 deficiency is linked to improper neuronal-glial interaction in radial migration Nature Neuroscience, Volume 6, Number 12, Pages 1284-1291, December 2003 Hidenori Tabata and Kazunori Nakajima Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex The Journal of Neuroscience, Volume 23, Issue 31, Pages 9996-10001, 5 November 2003 Xie et al. Serine 732 Phosphorylation of FAK by Cdk5 Is Important for Microtubule Organization, Nuclear Movement, and Neuronal Migration Cell, Volume 114, Issue 4, Pages 469-482, 22 August 2003 Tomomi Fukuchi-Shimogori and Elizabeth A Grove Emx2 patterns the neocortex by regulating FGF positional signaling Nature Neuroscience, Volume 6, Number 8, Pages 825-831, August 2003 Ohtsuka et al. Roles of the Basic Helix-Loop-Helix Genes Hes1 and Hes5 in Expansion of Neural Stem Cells of the Developing Brain The Journal of Biological Chemistry, Volume 276, Issue 32, Pages 30467-30474, 10 August 2001 H. Tabata and K. Nakajima Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex Neuroscience, Volume 103, Issue 4, Pages 865-872, 4 April 2001

Mouse/Rat Embryos cultured	Koike S, Keino-Masu K, Ohto T, Sugiyama F, Takahashi S, Masu M. Autotaxin/lysophospholipase D-mediated lysophosphatidic acid signaling is required to form distinctive large lysosomes in the visceral endoderm cells of the mouse yolk sac. J Biol Chem. 2009 Nov 27;284(48):33561-70. Okada T, Okumura Y, Motoyama J, Ogawa M. FGF8 signaling patterns the telencephalic midline by regulating putative key factors of midline development. Dev Biol. 2008 Aug 1;320(1):92-101. Shibuya et al. Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin Arteriosclerosis, Thrombosis, and Vascular Biology, Volume 26, Issue 5, Pages 1051-1057, May 2006 Tadashi Nomura and Noriko Osumi Misrouting of mitral cell progenitors in the Pax6/small eyerat telencephalon Development, Volume 131, Issue 4, Pages 787-796, February 2004 Kawabata et al. Electroporation-mediated gene transfer system applied to cultured CNS neurons Neuroreport, Volume 15, Issue 6, Pages 971-975, 29 April 2004 Masanori Takahashi and Noriko Osumi Pax6 regulates specification of ventral neurone subtypes in the hindbrain by establishing progenitor domains Development, Volume 129, Issue 6, Pages 1327-1338, March 2002 Takahashi et al. Manipulating gene expressions by electroporation in the developing brain of mammalian embryos Differentiation, Volume 70, Issue 4-5, Pages 155-162, June 2002 Noriko Osumi and Takayoshi Inoue Gene Transfer into Cultured Mammalian Embryos by Electroporation Methods, Volume 24, Issue 1, Pages 35-42, May 2001

Mouse/Rat Fertilized eggs & 8-16-cell stage embryos	Ohnishi Y, Totoki Y, Toyoda A, Watanabe T, Yamamoto Y, Tokunaga K, Sakaki Y, Sasaki H, Hohjoh H Small RNA class transition from siRNA/piRNA to miRNA during pre-implantation mouse development Nucleic Acids Res. 2010 Aug;38(15):5141-51.

Mouse/Rat Endometrium	Ryosuke Kobayashi, Reika Kawabata-Iwakawa, Makoto Sugiyama, Tetsunari Oyama, Masato Ohtsuka, Takuro Horii, Sumiyo Morita, Masahiko Nishiyama, Izuho Hatada Multiplexed genome editing by in vivo electroporation of Cas9 ribonucleoproteins effectively induces endometrial carcinoma in mice Int J Cancer. 2022 Nov 15. Kobayashi R, Endo K, Ohmori Y, Hondo E A novel method of gene transduction to the murine endometrium using in vivo electroporation. J Vet Med Sci. 2017 Sep 29;79(9):1573-1577.

Mouse/Rat Brain tissues ex vivo	Jin Cheng, Sadhna Sahani, Torben Johann Hausrat, Jenq-Wei Yang, Haichao Ji, Nikolai Schmarowski, Heiko Endle, Xinfeng Liu, Yunbo Li, Rahel Böttche, Konstantin Radyushkin, Hans M Maric, Anna Hoerder-Suabedissen, Zoltán Molnár, Pierre-Hugues Prouvot, Thorsten Trimbuch, Olaf Ninnemann, Jisen Huai, Wei Fan, Barbara Visentin, Roger Sabbadini, Kristian Strømgaard, Albrecht Stroh, Heiko J Luhmann, Matthias Kneussel, Robert Nitsch, Johannes Vogt Precise Somatotopic Thalamocortical Axon Guidance Depends on LPA-Mediated PRG-2/Radixin Signaling Neuron, 92 (1), 126-142 2016 Oct 5 Oshikawa M et al. Cortical excitatory neurons become protected from cell division during neurogenesis in an Rb family-dependent manner. Development. 2013 Jun;140(11):2310-20. Hutchins BI et al. Wnt/calcium signaling mediates axon growth and guidance in the developing corpus callosum. Dev Neurobiol. 2011 Apr;71(4):269-83. Tanaka M et al. Homer proteins control neuronal differentiation through IP(3) receptor signaling. FEBS Lett. 2006 Nov 13;580(26):6145-50. Yozu et al. The Caudal Migratory Stream: A Novel Migratory Stream of Interneurons Derived from the Caudal Ganglionic Eminence in the Developing Mouse Forebrain The Journal of Neuroscience, Volume 25, Issue 31, Pages 7268-7277, 3 August 2005 Kawabata et al. Electroporation-mediated gene transfer system applied to cultured CNS neurons Neuroreport, Volume 15, Issue 6, Pages 971-975, 29 April 2004

Mouse/Rat Brain neonatal in vivo	Hiromi Yamamoto, Keisuke Yamamura, Haruka Nagasaki, Takamasa Suzuki, Fumiko Ninomiya, Kenji Matsubara, Naomoto Harada, Shuichi Ohkubo Genome editing of Nf1, Pten, and Trp53 in neonatal mice induces glioblastomas positive for oligodendrocyte lineage transcription factor 2 J Toxicol Pathol. 2021 Oct;34(4):359-365. Julie Stoufflet, Maxime Chaulet, Mohamed Doulazmi, Coralie Fouquet, Caroline Dubacq, Christine Métin, Alain Trembleau, Pierre Vincent, Isabelle Caillé Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration bioRxiv November 12, 2019 Renaud et al. Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells Nature Neuroscience, Volume 11, Number 4, Pages 440-449, April 2008 Boutin et al. Efficient In Vivo Electroporation of the Postnatal Rodent Forebrain PLoS ONE, Volume 3, Issue 4, e1883, 2 April 2008 Umeshima et al. Microtubule-based nuclear movement occursindependently of centrosome positioning in migrating neurons PNAS, Volume 104, Number 41, Pages 16182-16187, October 9, 2007

Mouse/Rat Brain adult in vivo	Julie Stoufflet, Maxime Chaulet, Mohamed Doulazmi, Coralie Fouquet, Caroline Dubacq, Christine Métin, Alain Trembleau, Pierre Vincent, Isabelle Caillé Primary cilium-dependent cAMP/PKA signaling at the centrosome regulates neuronal migration bioRxiv November 12, 2019 Hidenori Ito, Rika Morishita, Makoto Mizuno, Hidenori Tabata, Koh-Ichi Nagata Rho Family GTPases, Rac and Cdc42, Control the Localization of Neonatal Dentate Granule Cells During Brain Development Hippocampus, 29 (7), 569-578 Jul 2019 Elodie Gaborieau, Anahi Hurtado-Chong, Maria Fernández, Kasum Azim, Olivier Raineteau A Dual Role for the Transcription Factor Sp8 in Postnatal Neurogenesis Sci Rep, 8 (1), 14560 2018 Sep 28 Hiroshi Ueda Cellular Mechanismhs Underlying Morphine Analgesic Tolerance and Hyperalgesia Opioid-Induced Hyperalgesia, First, Pages 9-20, October 2009 Barnabe-Heider et al. Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation Nature Methods, Volume 5, Number 2, Pages 189-196, February 2008 Inoue et al. Locus-Specific Rescue of GluR1 NMDA Receptors in Mutant Mice Identifies the Brain Regions Important for Morphine Tolerance and Dependence The Journal of Neuroscience, Volume 23, Issue 16, Pages 6529-6536, 23 July 2003 Tanaka et al. Up-regulation of Protein-disulfide Isomerase in Response to Hypoxia/Brain Ischemia and Its Protective Effect against Apoptotic Cell Death The Journal of Biological Chemistry, Volume 275, Issue 14, Pages 10388-10393, 7 April 2000

Mouse/Rat Retina in vivo/ex vivo	Takei A, Ekström M, Mammadzada P, Aronsson M, Yu M, Kvanta A, André H Gene Transfer of Prolyl Hydroxylase Domain 2 Inhibits Hypoxia-inducible Angiogenesis in a Model of Choroidal Neovascularization. Sci Rep. 2017 Feb 10;7:42546. Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Sugitani K, Arai K, Suzuki H, Kato S. Requirement of retinoic acid receptor β for genipin derivative-induced optic nerve regeneration in adult rat retina. PLoS One. 2013 Aug 6;8(8):e71252. Tracy L. Young and Constance L. Cepko A role for ligand-gated ion channels in rod photoreceptor development Neuron, Volume 41, Issue 6, Pages 867-879, 25 March 2004 Takahiko Matsuda and Constance L. Cepko Electroporation and RNA interference in the rodent retina in vivo and in vitro PNAS, Volume 101, Number 1, Pages 16-22, 6 January 2004 Dezawa et al. Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach Micron, Volume 33, Issue 1, Pages 1-6, 2002

Mouse/Rat Retina ex vivo	Benjamin Souferi, Mark M Emerson Quantitative Analysis of the ThrbCRM1-centered Gene Regulatory Network Biol Open, 8 (4) 2019 Apr 26 Mark M Emerson, Constance L Cepko Identification of a Retina-Specific Otx2 Enhancer Element Active in Immature Developing Photoreceptors Dev Biol, 360 (1), 241-55 2011 Dec 1 Briggman KL, Euler T. Bulk electroporation and population calcium imaging in the adult mammalian retina. J Neurophysiol. 2011 May;105(5):2601-9. Ozawa Y et al. Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation. J Biol Chem. 2008 Sep 5;283(36):24561-70.

Mouse/Rat Cornea in vivo	Oshima et al., Targeted Gene Transfer to Corneal Stroma in vivo by Electric Pulses Experimental Eye Research, Volume 74, Issue 2, Pages 191-198, February 2002

Mouse/Rat Ear in vivo	Tomomi Yamamoto-Fukuda, Naotaro Akiyama, Hiromi Kojima Keratinocyte Growth Factor (KGF) Induces Stem/Progenitor Cell Growth in Middle Ear Mucosa Int J Pediatr Otorhinolaryngol, 128, 109699 Jan 2020 Tomomi Yamamoto-Fukuda, Naotaro Akiyama, Yasuaki Shibata, Haruo Takahashi, Tohru Ikeda, Takehiko Koji In Vivo Over-Expression of KGF Mimic Human Middle Ear Cholesteatoma Eur Arch Otorhinolaryngol, 272 (10), 2689-96 Oct 2015

Mouse/Rat Sciatic Nerve in vivo	Alejandra Catenaccio, Maica Llavero Hurtado, Paula Diaz, Douglas J Lamont, Thomas M Wishart, Felipe A Court Molecular analysis of axonal-intrinsic and glial-associated co-regulation of axon degeneration Cell Death Dis. 2017 Nov 9;8(11):e3166. Lopez-Verrilli MA et al. Schwann cell-derived exosomes enhance axonal regeneration in the peripheral nervous system. Glia. 2013 Nov;61(11):1795-806.

Mouse/Rat Spinal Cord in vivo	Po-Kuan Chao, Hsiao-Fu Chang, Li-Chin Ou, Jian-Ying Chuang, Pin-Tse Lee, Wan-Ting Chang, Shu-Chun Chen, Shau-Hua Ueng, John Tsu-An Hsu, Pao-Luh Tao, Ping-Yee Law, Horace H Loh, Shiu-Hwa Yeh Convallatoxin Enhance the Ligand-Induced Mu-Opioid Receptor Endocytosis and Attenuate Morphine Antinociceptive Tolerance in Mice Sci Rep, 9 (1), 2405 2019 Feb 20 Lee PT, Chao PK, Ou LC, Chuang JY, Lin YC, Chen SC, Chang HF, Law PY, Loh HH, Chao YS, Su TP, Yeh SH Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling. Nucleic Acids Res. 2014 Dec 1;42(21):13012-25. Akimoto N et al. CCL-1 in the spinal cord contributes to neuropathic pain induced by nerve injury. Cell Death Dis. 2013 Jun 20

Mouse/Rat Spinal Cord ex vivo	Mariko Kinoshita-Kawada, Hiroshi Hasegawa, Tsunaki Hongu, Shigeru Yanagi, Yasunori Kanaho, Ichiro Masai, Takayasu Mishima, Xiaoping Chen, Yoshio Tsuboi,Yi Rao, Junichi Yuasa-Kawada, Jane Y. Wu Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation bio-protocol Vol 9, Iss 18, September 20, 2019 Kinoshita-Kawada M, Hasegawa H, Hongu T, Yanagi S, Kanaho Y, Masai I, Mishima T, Chen X, Tsuboi Y, Rao Y, Yuasa-Kawada J, Wu JY A crucial role for Arf6 in the response of commissural axons to Slit. Development. 2019 Feb 4;146(3).

Mouse/Rat Lung in vivo	Muliawan HS, Nakayama K, Yagi K, Ikeda K, Yagita K, Hirata K, Emoto N Stable Somatic Gene Expression in Mouse Lungs Following Electroporation-mediated Tol2 Transposon Delivery. Kobe J Med Sci. 2015 Oct 7;61(2):E47-53.

Mouse/Rat Spleen in vivo	Guan X et al. Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination. The Journal of Gene Medicine, Volume 12, Issue 3, Pages 301-309, March 2010

Mouse/Rat Pancreas in vivo	Masahiro Sato, Emi Inada, Issei Saitoh, Shingo Nakamura, Satoshi Watanabe In Vivo Piggy bac-Based Gene Delivery Towards Murine Pancreatic Parenchyma Confers Sustained Expression of Gene of Interest Int J Mol Sci, 20 (13) 2019 Jun 26 Engin Gürlevik, Bettina Fleischmann-Mundt, Jennifer Brooks, Ihsan Ekin Demir, Katja Steiger, Silvia Ribback, Tetyana Yevsa, Norman Woller, Arnold Kloos, Dmitrij Ostroumov, Nina Armbrecht, Michael P Manns, Frank Dombrowski, Michael Saborowski, Moritz Kleine, Thomas C Wirth, Helmut Oettle, Güralp O Ceyhan, Irene Esposito, Diego F Calvisi, Stefan Kubicka, Florian Kühnel Administration of Gemcitabine After Pancreatic Tumor Resection in Mice Induces an Antitumor Immune Response Mediated by Natural Killer Cells Gastroenterology, 151 (2), 338-350.e7 Aug 2016 Maresch R, Mueller S, Veltkamp C, Öllinger R, Friedrich M, Heid I, Steiger K, Weber J, Engleitner T, Barenboim M, Klein S, Louzada S, Banerjee R, Strong A, Stauber T, Gross N, Geumann U, Lange S, Ringelhan M, Varela I, Unger K, Yang F, Schmid RM, Vassiliou GS, Braren R, Schneider G, Heikenwalder M, Bradley A, Saur D, Rad R Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice. Nat Commun. 2016 Feb 26;7 Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice. Biotechnol J. 2013 Aug 15. [Epub ahead of print] Guan X1, Nishikawa M, Takemoto S, Ohno Y, Yata T, Takakura Y Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination. J Gene Med. 2010 Mar;12(3):301-9.

Mouse/Rat Islets of langerhans ex vivo	Mica Ohara-Imaizumi, Kyota Aoyagi, Hajime Yamauchi, Masashi Yoshida, Masayuki X Mori, Yamato Hida, Ha Nam Tran Masamichi Ohkura, Manabu Abe, Yoshihiro Akimoto, Yoko Nakamichi, Chiyono Nishiwaki, Hayato Kawakami, Kazuo Hara, Kenji Sakimura, Shinya Nagamatsu, Yasuo Mori, Junichi Nakai, Masafumi Kakei, Toshihisa Ohtsuka ELKS/Voltage-Dependent Ca 2+ Channel-β Subunit Module Regulates Polarized Ca 2+ Influx in Pancreatic β Cells Cell Rep, 26 (5), 1213-1226.e7 2019 Jan 29 Ohara-Imaizumi. et al. Imaging of Insulin Exocytosis from Pancreatic Beta Cells. Exocytosis Methods, Neuromethods Volume 83, 2014 Aoyagi K. et al. Acute inhibition of PI3K-PDK1-Akt pathway potentiates insulin secretion through upregulation of newcomer granule fusions in pancreatic β-cells. PLoS One. 2012;7(10):e47381.

Mouse/Rat Liver in vivo	Higuchi S, Fujikawa R, Nakatsuji M, Yasui M, Ikedo T, Nagata M, Mishima K, Irie K, Matsumoto M, Yokode M, Minami M EP4 receptor-associated protein (EPRAP) regulates gluconeogenesis in the liver and is associated with hyperglycemia in diabetic mice. Am J Physiol Endocrinol Metab. 2018 Dec 18 Seehawer M, Heinzmann F, D'Artista L, Harbig J, Roux PF, Hoenicke L, Dang H, Klotz S, Robinson L, Doré G, Rozenblum N, Kang TW, Chawla R, Buch T, Vucur M, Roth M, Zuber J, Luedde T, Sipos B, Longerich T, Heikenwälder M, Wang XW, Bischof O, Zender L Necroptosis microenvironment directs lineage commitment in liver cancer. Nature. 2018 Sep 12. doi: 10.1038/s41586-018-0519-y. [Epub ahead of print] Gürlevik E et al. Adjuvant gemcitabine therapy improves survival in a locally induced, R0-resectable model of metastatic intrahepatic cholangiocarcinoma. Hepatology. 2013 Sep;58(3):1031-41. Zang et al. Inhibition of nuclear delivery of plasmid DNA and transcription by interferon γ: hurdles to be overcome for sustained gene therapy. Gene Ther. 2011 Sep;18(9):891-7. Kiyama S et al. Reduction of fibrosis in a rat model of non-alcoholic steatohepatitis cirrhosis by human HGF gene transfection using electroporation. J Gastroenterol Hepatol. 2008 Aug;23(8 Pt 2):e471-6. Nakagawa et al. Deubiquitylation of histone H2A activates transcriptiona initiation via trans-histone cross-talk with H3K4 di- and trimethylation Genes & Development, Volume 22, Issue 1, Pages 37-49, 1 January 2008 Kawano T et al. Stabilizing of plasmid DNA in vivo by PEG-modified cationic gold nanoparticles and the gene expression assisted with electrical pulses. J Control Release. 2006 Apr 10;111(3):382-9. Sakai et al. Hepatocyte-targeted gene transfer by combination of vascularly delivered plasmid DNA and in vivo electroporation. Gene Therapy, Volume 12, Issue 7, Pages 607-616, April 2005

Mouse/Rat Kidney in vivo	Ma et al. Local Actions of Endogenous Angiotensin II in Injured Glomeruli Journal of the American Society of Nephrology, Volume 15, Issue 5, Pages 1268-1276, May 2004 Yazawa et al. Direct transfer of hepatocyte growth factor gene into kidney suppresses cyclosporin A nephrotoxicity in rats Nephrology Dialysis Transplantation, Volume 19, Issue 4, Pages 812-816, April 2004

Mouse/Rat Colon in vivo	Choijookhuu N et al. Estrogen-dependent regulation of sodium/hydrogen exchanger-3 (NHE3) expression via estrogen receptor β in proximal colon of pregnant mice. Histochem Cell Biol. 2012 May;137(5):575-87.

Mouse/Rat Gonad ex vivo	Laura Bellutti, Emilie Abby, Sophie Tourpin, Sébastien Messiaen, Delphine Moison, Emilie Trautmann, Marie-Justine Guerquin, Virginie Rouiller-Fabre, René Habert, Gabriel Livera Divergent Roles of CYP26B1 and Endogenous Retinoic Acid in Mouse Fetal Gonads. Biomolecules, 9 (10) 2019 Sep 26

Mouse/Rat Prostate	Josef Leibold, Marcus Ruscetti, Zhen Cao, Yu-Jui Ho, Timour Baslan, Min Zou, Wassim Abida, Judith Feucht, Teng Han, Francisco M Barriga, Kaloyan M Tsanov, Leah Zamechek, Amanda Kulick, Corina Amor, Sha Tian, Katarzyna Rybczyk, Nelson R Salgado, Francisco Sánchez-Rivera, Philip A Watson, Elisa de Stanchina, John E Wilkinson, Lukas E Dow, Cory Abate-Shen, Charles L Sawyers, Scott W Lowe Somatic Tissue Engineering in Mouse Models Reveals an Actionable Role for WNT Pathway Alterations in Prostate Cancer Metastasis Cancer Discov. 2020 May 6.

Mouse/Rat Testis in vivo	Frantzeskos Papanikos, Katrin Daniel, Angelique Goercharn-Ramlal, Ji-Feng Fei, Thomas Kurth, Lukasz Wojtasz, Ihsan Dereli, Jun Fu, Josef Penninger, Bianca Habermann, Azim Surani, A Francis Stewart, Attila Toth The Enigmatic Meiotic Dense Body and Its Newly Discovered Component, SCML1, Are Dispensable for Fertility and Gametogenesis in Mice Chromosoma, 126 (3), 399-415 Jun 2017 Michaelis M, Sobczak A, Weitzel JM. In Vivo Microinjection and Electroporation of Mouse Testis. J Vis Exp. 2014 Aug 23;(90). Somboonthum P, Ohta H, Yamada S, Onishi M, Ike A, Nishimune Y, Nozaki M. cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis Nucleic Acids Res. 2005 Jun 10;33(10):3401-11. Ike A et al., Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation FEBS Letters, Volume 559, Issues 1-3, Pages 159-164, 13 February 2004 Yomogida K et at., Dramatic Expansion of Germinal Stem Cells by Ectopically Expressed Human Glial Cell Line-Derived Neurotrophic Factor in Mouse Sertoli Cells Biology of Reproduction, Volume 69, Issue 4, Pages 1303-1307, 2003 Hisano et al. Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells Nucleic Acids Research, Volume 31, Number 16, Pages 4797-4804, 15 August 2003 Yomogida et al. Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect Biology of Reproduction, Volume 67, Issue 3, Pages 712-717, September 2002

Mouse/Rat Ovary in vivo	Stella V Paffenholz, Camilla Salvagno, Yu-Jui Ho, Matthew Limjoco, Timour Baslan, Sha Tian, Amanda Kulick, Elisa de Stanchina, John E Wilkinson, Francisco M Barriga, Dmitriy Zamarin, Juan R Cubillos-Ruiz, Josef Leibold, Scott W Lowe Senescence induction dictates response to chemo- and immunotherapy in preclinical models of ovarian cancer Proc Natl Acad Sci U S A. 2022 Feb 1;119(5):e2117754119. Suzuki et al. Effect of CaCl₂ concentration on the rate of foreign gene transfer and expression by in vivo electroporation in the mouse ovary International Journal of Molecular Medicine, Volume 12, Number 3, Pages 365-358, September 2003

Mouse/Rat Muscle in vivo	Keigo Yato, Mami Matsuda, Kento Fukano, Tomohisa Tanaka, Kohji Moriishi, Hironori Nishitsuji, Kunitada Shimotohno, Koji Tamura, Takaji Wakita, Masamichi Muramatsu, Takanobu Kato, Ryosuke Suzuki Identification of neutralizing epitopes in the preS2 domain of the hepatitis B virus Virus Res. 2023 Jan 2;323:199014. Bruna Felício Milazzotto Maldonado Porchia, Luana Raposo de Melo Moraes Aps, Ana Carolina Ramos Moreno, Jamile Ramos da Silva, Mariângela de Oliveira, Natiely Silva Sales, Rubens Prince Dos Santos Alves, Clarissa Ribeiro Reily Rocha, Matheus Molina Silva, Karine Bitencourt Rodrigues, Tácita Borges Barros, Roberta Liberato Pagni, Patrícia da Cruz Souza, Mariana de Oliveira Diniz, Luís Carlos de Souza Ferreira Active immunization combined with cisplatin confers enhanced therapeutic protection and prevents relapses of HPV-induced tumors at different anatomical sites Int J Biol Sci. 2022 Jan 1;18(1):15-29. Lennon Ramos Pereira, Rúbens Prince dos Santos Alves, Natiely Silva Sales, Robert Andreata-Santos, Aléxia Adrianne Venceslau-Carvalho, Samuel Santos Pereira, Maria Fernanda Castro-Amarante1, Mônica Josiane Rodrigues-Jesus, Marianna Teixeira de Pinho Favaro, Rosa Maria Chura-Chambi, Ligia Morganti and Luís Carlos de Souza Ferreira Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein Front. Med. Technol., 03 December 2020 Kun Huang, Jin Li, Mikako Ito, Jun-Ichi Takeda, Bisei Ohkawara, Tomoo Ogi, Akio Masuda, Kinji Ohno Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle Front Mol Neurosci. 2020 Sep 8;13:154. Maria Carmela Filomena, Daniel L Yamamoto, Marco Caremani, Vinay K Kadarla, Giuseppina Mastrototaro, Simone Serio, Anupama Vydyanath, Margherita Mutarelli, Arcamaria Garofalo, Irene Pertici, Ralph Knöll, Vincenzo Nigro, Pradeep K Luther, Richard L Lieber, Moriah R Beck, Marco Linari, Marie-Louise Bang Myopalladin Promotes Muscle Growth Through Modulation of the Serum Response Factor Pathway J Cachexia Sarcopenia Muscle, 11 (1), 169-194 Feb 2020 V. V. Shilov, M. A. Yudin, N. G. Vengerovich, T. V. Shcherbakov, A. S. Bogacheva Bioavailability Study of Recombinant Plasmid DNA Nerve Growth Factor after Intramuscular Injection by Electroporation МЕДИЦИНА, Т. 14. Вып. 2, pp. 89–97 14 Nov 2019 Jamile R Silva, Natiely S Sales, Mariângela O Silva, Luana R M M Aps, Ana C R Moreno, Elaine G Rodrigues, Luís C S Ferreira, Mariana O Diniz Expression of a Soluble IL-10 Receptor Enhances the Therapeutic Effects of a Papillomavirus-Associated Antitumor Vaccine in a Murine Model Cancer Immunol Immunother, 68 (5), 753-763 May 2019 Lynette J Oost, Monika Kustermann, Andrea Armani, Bert Blaauw, Vanina Romanello Fibroblast Growth Factor 21 Controls Mitophagy and Muscle Mass J Cachexia Sarcopenia Muscle, 10 (3), 630-642 Jun 2019 Atsushi Yamanaka, Eiji Konishi Key Amino Acid Substitution for Infection-Enhancing Activity-Free Designer Dengue Vaccines iScience. 2019 Mar 29;13:125-137. Satoshi Nagamata, Taiki Aoshi, Akiko Kawabata, Yoshiaki Yamagishi, Mitsuhiro Nishimura, Soichiro Kuwabara, Kouki Murakami, Hideto Yamada, Yasuko Mori Identification of CD4 and H-2K d-restricted Cytotoxic T Lymphocyte Epitopes on the Human Herpesvirus 6B Glycoprotein Q1 Protein Sci Rep, 9 (1), 3911 2019 Mar 7 Nobuyuki Takenaka, Natsumi Araki, Takaya Satoh Involvement of the Protein Kinase Akt2 in Insulin-Stimulated Rac1 Activation Leading to Glucose Uptake in Mouse Skeletal Muscle PLoS One, 14 (2), e0212219 2019 Feb 8 eCollection 2019 Arthur Baruel Zaneti, Marcio Massao Yamamoto, Fernando Bandeira Sulczewski, Bianca da Silva Almeida, Higo Fernando Santos Souza, Natália Soares Ferreira, Denicar Lina Nascimento Fabris Maeda, Natiely Silva Sales, Daniela Santoro Rosa, Luís Carlos de Souza Ferreira, Silvia Beatriz Boscardin Dendritic Cell Targeting Using a DNA Vaccine Induces Specific Antibodies and CD4 + T Cells to the Dengue Virus Envelope Protein Domain III Front Immunol, 10, 59 2019 Jan 29 eCollection 2019 Xiang Li, Jane Baker, Tobias Cracknell, Andrew R Haynes, Gonzalo Blanco IGFN1_v1 Is Required for Myoblast Fusion and Differentiation PLoS One, 12 (6), e0180217 2017 Jun 30 eCollection 2017 Atsushi Yamanaka, Pannamthip Pitaksajjakul, Pongrama Ramasoota, Eiji Konishi Expression of Enhancing-Activity-Free Neutralizing Antibody Against Dengue Type 1 Virus in Plasmid-Inoculated Mice Vaccine, 33 (45), 6070-7 2015 Nov 9 Matsuura M et al. Novel polyvalent live vaccine against varicella-zoster and mumps virus infections. Microbiol Immunol. 2013 Oct;57(10):704-14. Kawai M, Bessho K, Maruyama H, Miyazaki J, Yamamoto T Simultaneous gene transfer of bone morphogenetic protein (BMP) -2 and BMP-7 by in vivo electroporation induces rapid bone formation and BMP-4 expression. BMC Musculoskelet Disord. 2006 Aug 3;7:62. Takeshita et al. Toll-Like Receptor Adaptor Molecules Enhance DNA-Raised Adaptive Immune Responses against Influenza and Tumors through Activation of Innate Immunity Journal of Virology, Volume 80, Number 13, Pages 6218-6224, July 2006 Saba et al. Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosa Vaccine, Volume 24, Issues 37-39, Pages 6240-6249, 11 September 2006 Kitaguchi et al. Immune deficiency enhances expression of recombinant human antibody in mice after nonviral in vivo gene transfer International Journal of Molecular Medicine, Volume 16, Number 4, Pages 683-688, October 2005 Umeda et al. Skeletal muscle targeting in vivo electroporation-mediated HGF gene therapy of bleomycin-induced pulmonary fibrosis in mice Laboratory Investigation, Volume 84, Issue 7, Pages 836-844, July 2004 Yoshida et al. Gene Therapy for Central Diabetes Insipidus: Effective Antidiuresis by Muscle-Targeted Gene Transfer Endocrinology, Volume 145, Number 1, Pages 261-268, January 2004 Xue et al. Hepatocyte growth factor gene therapy accelerates regeneration in cirrhotic mouse livers after hepatectomy Gut, Volume 52, Number 5, Pages 694-700, May 2003 Kawai et al. Ectopic Bone Formation by Human Bone Morphogenetic Protein-2 Gene Transfer to Skeletal Muscle Using Transcutaneous Electroporation Human Gene Therapy, Volume 14, Number 16, Pages 1547-1556, November 2003 Inoue et al. Anti-Monocyte Chemoattractant Protein-1 Gene Therapy Limits Progression and Destabilization of Established Atherosclerosis in Apolipoprotein E–Knockout Mice Circulation, Volume 106, Issue 21, Pages 2700-2706, 19 November 2002 Ikeda et al. Anti-monocyte chemoattractant protein-1 gene therapy attenuates pulmonary hypertension in rats American Journal of Physiology - Heart and Circulatory Physiology, Volume 283, Issue 5, Pages H2021-H2028, November 2002 Xue et al. Attenuated acute liver injury in mice by naked hepatocyte growth factor gene transfer into skeletal muscle with electroporation Gut, Volume 50, Number 4, Pages 558-562, April 2002 Tanaka et al. Gene Therapy for Mitochondrial Disease by Delivering Restriction Endonuclease SmaI into Mitochondria Journal of Biomedical Science, Volume 9, Number 6, Pages 534-541, 2002 Takada et al. Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein Journal of Virology, Volume 75, Number 5, Pages 2324-2330, March 2001 Watanabe et al. Protection Against Autoimmune Myocarditis by Gene Transfer of Interleukin-10 by Electroporation Circulation, Volume 104, Issue 10, Pages 1098-1100, 4 September 2001 Yasui et al. Elevated gastrin secretion by in vivo gene electroporation in skeletal muscle International Journal of Molecular Medicine, Volume 8, Number 5, Pages 489-494, November 2001

Mouse/Rat Skin in vivo	Guan X et al. Injection site-dependent induction of immune response by DNA vaccine: comparison of skin and spleen as a target for vaccination. The Journal of Gene Medicine, Volume 12, Issue 3, Pages 301-309, March 2010 Inoue et al. Modulation of scratching behavior by silencing an endogenou cyclooxygenase-1 gene in the skin through the administration of siRNA The Journal of Gene Medicine, Volume 9, Issue 11, Pages 994-1001, November 2007 Maruyama et al. Epidermis-Targeted Gene Transfer Using In Vivo Electroporation Epidermal Cells Methods and Protocols, Series: Methods in Molecular Biology, Volume 289, Pages 431-436, October 2004

Mouse/Rat Joint in vivo	Saito et al. Sonoporation mediated transduction of pDNA/siRNA into joint synovium in vivo Journal of Orthopaedic Research, Volume 25, Issue 10, Pages 1308-1316, October 2007 Inoue et al. Electro-transfer of small interfering RNA ameliorated arthritis in rats Biochemical and Biophysical Research Communications, Volume 336, Issue 3, Pages 903-908, 28 October 2005 Ohashi et al. Successful genetic transduction in vivo into synovium by means of electroporation Biochemical and Biophysical Research Communications, Volume 293, Issue 5, Pages 1530-1535, 24 May 2002

Mouse/Rat Cartilage ex vivo	Katayama et al. Efficient gene delivery to articular cartilage using electroporation MODERN RHEUMATOLOGY, Volume 13, Number 3, 243-249, 2003

Mouse/Rat Liver, Kidney, Spleen, Skin & Muscle in vivo	Thanaketpaisarn et al. Tissue-Specific Characteristics of in Vivo Electric Gene: Transfer by Tissue and Intravenous Injection of Plasmid DNA Pharmaceutical Research, Volume 22, Number 6, Pages 883-891, June 2005

Mouse/Rat Tumors in vivo	Jee Young Sung, Kyungsil Yoon, Sang-Kyu Ye, Sung-Ho Goh, Seog-Yun Park, June Hyuk Kim, Hyun Guy Kang, Yong-Nyun Kim, Byung-Kiu Park Upregulation of Transforming Growth Factor-Beta Type I Receptor by Interferon Consensus Sequence-Binding Protein in Osteosarcoma Cells Biochim Biophys Acta Mol Cell Res, 1866 (5), 761-772 May 2019 Ji Hae Lee, Jee Young Sung, Eun Kyung Choi, Hyun-Kyoung Yoon, Bo Ram Kang, Eun Kyung Hong, Byung-Kiu Park, Yong-Nyun Kim, Seung Bae Rho, Kyungsil Yoon C/EBPβ Is a Transcriptional Regulator of Wee1 at the G₂/M Phase of the Cell Cycle Cells, 8 (2) 2019 Feb 11 Takei Y Electroporation-mediated siRNA delivery into tumors. Methods Mol Biol. 2014;1121:131-8. Shibata MA et al. The endogenous soluble VEGF receptor-2 isoform suppresses lymph node metastasis in a mouse immunocompetent mammary cancer model. BMC Med. 2010 Nov 3;8:69. Aung W et al. Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging. Gene Ther. 2009 Jul;16(7):830-9. Shibata MA et al. Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model. Cancer Gene Ther. 2008 Dec;15(12):776-86. Kang JH Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex Cancer Letters, Volume 265, Issue 2, Pages 281-288, 8 July 2008 Takei Y et al. In vivo silencing of a molecular target by short interfering RNA electroporation: tumor vascularization correlates to delivery efficiency Molecular Cancer Therapeutics, Volume 7, Issue 1, Pages 211-221, 1 January, 2008

Human Zygotes	Claudia Gerri, Afshan McCarthy, Gregorio Alanis-Lobato, Andrej Demtschenko, Alexandre Bruneau, Sophie Loubersac, Norah M E Fogarty, Daniel Hampshire, Kay Elder, Phil Snell, Leila Christie, Laurent David, Hilde Van de Velde, Ali A Fouladi-Nashta, Kathy K Niakan Initiation of a conserved trophectoderm program in human, cow and mouse embryos Nature. 2020 Nov;587(7834):443-447

Monkey Zygotes embryos	Nanami Kohri, Mitsuo Ota, Hikaru Kousaku, Eiko N. Minakawa, Kazuhiko Seki, Ikuo Tomioka Optimization of piggyBac transposon-mediated gene transfer method in common marmoset embryos PLoS One. 2023 Jun 9;18(6):e0287065. Tomoyuki Tsukiyama, Kenichi Kobayashi, Masataka Nakaya, Chizuru Iwatani, Yasunari Seita, Hideaki Tsuchiya, Jun Matsushita, Kahoru Kitajima, Ikuo Kawamoto, Takahiro Nakagawa, Koji Fukuda, Teppei Iwakiri, Hiroyuki Izumi, Iori Itagaki, Shinji Kume, Hiroshi Maegawa, Ryuichi Nishinakamura, Saori Nishio, Shinichiro Nakamura, Akihiro Kawauchi, Masatsugu Ema Monkeys Mutant for PKD1 Recapitulate Human Autosomal Dominant Polycystic Kidney Disease Nat Commun, 10 (1), 5517 2019 Dec 11 Yoshimatsu S, Okahara J, Sone T, Takeda Y, Nakamura M, Sasaki E, Kishi N, Shiozawa S, Okano H Robust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system. Sci Rep. 2019 Feb 6;9(1):1528.

Monkey Skin	Todorova B, Adam L, Culina S, Boisgard R, Martinon F, Cosma A, Ustav M, Kortulewski T, Le Grand R, Chapon C Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques. Sci Rep. 2017 Jun 23;7(1):4122. Pierre Roques, Karl Ljungberg, Beate M Kümmerer, Leslie Gosse, Nathalie Dereuddre-Bosquet, Nicolas Tchitchek, David Hallengärd, Juan García-Arriaza 6, Andreas Meinke, Mariano Esteban, Andres Merits, Roger Le Grand, Peter Liljeström Attenuated and Vectored Vaccines Protect Nonhuman Primates Against Chikungunya Virus JCI Insight. 2017 Mar 23;2(6):e83527. Adam L, Le Grand R, Martinon F. Electroporation-mediated intradermal delivery of DNA vaccines in nonhuman primates. Methods Mol Biol. 2014;1121:309-13.

Bovine Zygotes	Claudia Gerri, Afshan McCarthy, Gregorio Alanis-Lobato, Andrej Demtschenko, Alexandre Bruneau, Sophie Loubersac, Norah M E Fogarty, Daniel Hampshire, Kay Elder, Phil Snell, Leila Christie, Laurent David, Hilde Van de Velde, Ali A Fouladi-Nashta, Kathy K Niakan Initiation of a conserved trophectoderm program in human, cow and mouse embryos Nature. 2020 Nov;587(7834):443-447 Luiz Sergio Almeida Camargo1, Joseph R. Owen2, Alison L. Van Eenennaam and Pablo Juan Ross Efficient One-Step Knockout by Electroporation of Ribonucleoproteins Into Zona-Intact Bovine Embryos Front. Genet., 07 September 2020

Pig Zygotes	Shiro Yamashita, Yuhei Kogasaka, Yuuki Hiradate, Kentaro Tanemura, Yutaka Sendai Suppression of Mosaic Mutation by Co-Delivery of CRISPR Associated Protein 9 and Three-Prime Repair Exonuclease 2 Into Porcine Zygotes via Electroporation J Reprod Dev, 66 (1), 41-48 2020 Feb 14
Sheep Zygotes	Ahmed K Mahdi, Juan F Medrano, Pablo J Ross Single-Step Genome Editing of Small Ruminant Embryos by Electroporation Int J Mol Sci. 2022 Sep 6;23(18):10218.
Goat Zygotes	Ahmed K Mahdi, Juan F Medrano, Pablo J Ross Single-Step Genome Editing of Small Ruminant Embryos by Electroporation Int J Mol Sci. 2022 Sep 6;23(18):10218.

Hamster Embryos in oviduct (i-GONAD)	Michiko Hirose, Arata Honda, Helena Fulka, Miwa Tamura-Nakano, Shogo Matoba, Toshiko Tomishima, Keiji Mochida, Ayumi Hasegawa, Kiyoshi Nagashima, Kimiko Inoue, Masato Ohtsuka, Tadashi Baba, Ryuzo Yanagimachi, Atsuo Ogura Acrosin Is Essential for Sperm Penetration Through the Zona Pellucida in Hamsters Proc Natl Acad Sci USA, 117 (5), 2513-2518 2020 Feb 4

Chicken Embryos in ovo	Silvana Guioli, Debiao Zhao, Sunil Nandi, Michael Clinton, Robin Lovell-Badge Oestrogen in the Chick Embryo Can Induce Chromosomally Male ZZ Left Gonad Epithelial Cells to Form an Ovarian Cortex That Can Support Oogenesis Development, 147 (4) 2020 Feb 25 Yuji Atsuta, Reiko R Tomizawa, Michael Levin, Clifford J Tabin L-type Voltage-Gated Ca 2+ Channel Ca V 1.2 Regulates Chondrogenesis During Limb Development Proc Natl Acad Sci U S A, 116 (43), 21592-21601 2019 Oct 22 Tyler R Huycke, Bess M Miller, Hasreet K Gill, Nandan L Nerurkar, David Sprinzak, L Mahadevan, Clifford J Tabin Genetic and Mechanical Regulation of Intestinal Smooth Muscle Development Cell, 179 (1), 90-105.e21 2019 Sep 19 Ryo Egawa, Hiromu Yawo Analysis of Neuro-Neuronal Synapses Using Embryonic Chick Ciliary Ganglion via Single-Axon Tracing, Electrophysiology, and Optogenetic Techniques Curr Protoc Neurosci, 87 (1), e64 Apr 2019 Miruna G. Ghinia Tegla, Diego F. Buenaventura, Diana Y Kim, Cassandra Thakurdin, Kevin C. Gonzalez, Mark M Emerson Single cell profiling of CRISPR/Cas9-induced OTX2 deficient retinas reveals fate switch from restricted progenitors. bioRxiv. 2019 February 2, https://doi.org/10.1101/538710 Moreau C, Caldarelli P, Rocancourt D, Roussel J, Denans N, Pourquie O, Gros J Timed Collinear Activation of Hox Genes during Gastrulation Controls the Avian Forelimb Position. Curr Biol. 2019 Jan 7;29(1):35-50.e4. Sahasrabudhe A, Ghate K, Mutalik S, Jacob A, Ghose A Formin-2 regulates stabilization of filopodial tip adhesions in growth cones and affects neuronal outgrowth and pathfinding in vivo. Development. 2015 Dec 30. pii: dev.130104. [Epub ahead of print] Sanders TA, Llagostera E, Barna M. Specialized filopodia direct long-range transport of SHH during vertebrate tissue patterning. Nature. 2013 May 30;497(7451):628-32 Yuasa-Kawada et al. Midline crossing and Slit responsiveness of commissural axons require USP33 Nature Neuroscience, Volume 12, Number 9, Pages 1087-1089 Furne C et al. Netrin-1 is a survival factor during commissural neuron navigation. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14465-70. Towers et al. Integration of growth and specification in chick wing digit-patterning Nature, Volume 452, Number 7189, Pages 882-886, 17 April 2008 Chang et al. Bmp4 Is Essential for the Formation of the Vestibular Apparatus that Detects Angular Head Movements PLoS Genetics, Volume 4, Issue 4, e1000050, 11 April 2008 Sun et al. Expression dynamics of the LIM-homeobox genes, Lhx1 and Lhx9, in the diencephalon during chick development The International Journal of Developmental Biology, Volume 52, Number 1, Pages 33-41, 2008 Ladher et al. FGF8 initiates inner ear induction in chick and mouse Genes and Development, Volume 19, Issue 5, Pages 603-613, March 2005 Matsunaga et al. RGM and its receptor neogenin regulate neuronal survival Nature Cell Biology, Volume 6, Issue 8, Pages 749-755, August 2004 Nakamura et al. Gain- and loss-of-function in chick embryos by electroporation Mechanisms of Development, Volume 121, Issue 9, Pages 1137-1143, September 2004 Wakamatsu et al. Multiple roles of Sox2, an HMG-box transcription factor in avian neural crest development Developmental Dynamics, Volume 229, Issue 1, Pages 74-86, January 2004 Toyofuku et al., Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2 Genes & Development, Volume 18, Issue 4, Pages 435-447, 15 February 2004 Tatsuya Katahira and Harukazu Nakamura Gene silencing in chick embryos with a vector-based small interfering RNA system Development Growth & Differentiation, Volume 45, Issue 4, Pages 361-367, August 2003 Sayaka Sugiyama and Harukazu Nakamura The role of Grg4 in tectal laminar formation Development, Volume 130, Issue 3, Pages 451-462, February 2003 Sakiyama et al. Tbx4-Fgf10 system controls lung bud formation during chicken embryonic development Development, Volume 130, Issue 7, Pages 1225-1234, April 2003 Takeuchi et al. Tbx5 and Tbx4 trigger limb initiation through activation of the Wnt/Fgf signaling cascade Development, Volume 130, Issue 12, Pages 2729-2739, June 2003 Takeuchi at el. Tbx5 specifies the left/right ventricles and ventricular septum position during cardiogenesis Development, Volume 130, Issue 24, Pages 5953-5964, December 2003 Jin et al. Irx4-mediated regulation of Slit1 expression contributes to the definition of early axonal paths inside the retina Development, Volume 130, Issue 6, Pages 1037-1048, March 2003 C.-M. Amy Chen and Constance L. Cepko The chicken RaxL gene plays a role in the initiation of photoreceptor differentiation Development, Volume 129, Issue 23, Pages 5363-5375, December 2002 Treubert-Zimmemann et al. Targeting Axons to Specific Fiber Tracts In Vivo by Altering Cadherin Expression The Journal of Neuroscience, Volume 22, Issue 17, Pages 7617-7626, 1 September 2002 Oberg et al. Efficient ectopic gene expression targeting chick mesoderm Developmental Dynamics, Volume 224, Issue 3, Pages 291-302, July 2002 Sato et al. Inductive signal and tissue responsiveness defining the tectum and the cerebellum Development, Volume 128, Issue 13, Pages 2461-2469, July 2001 Nakamura et al. Misexpression of genes in brain vesicles by in ovo electroporation Development Growth & Differentiation, Volume 42, Issue 3, Page 199-201, June 2000 Itasaki et al. 'Shocking' developments in chick embryology: electroporation and in ovo gene expression Nature Cell Biology, Volume 1, Issue 8, Pages E203-E207, December 1999

Chicken Embryos ex ovo	Miruna G. Ghinia Tegla, Diego F. Buenaventura, Diana Y Kim, Cassandra Thakurdin, Kevin C. Gonzalez, Mark M Emerson Single cell profiling of CRISPR/Cas9-induced OTX2 deficient retinas reveals fate switch from restricted progenitors. bioRxiv. 2019 February 2, https://doi.org/10.1101/538710 Nandan L Nerurkar, ChangHee Lee, L Mahadevan, Clifford J Tabin Molecular control of macroscopic forces drives formation of the vertebrate hindgut Nature. 2019 Jan;565(7740):480-484. Moreau C, Caldarelli P, Rocancourt D, Roussel J, Denans N, Pourquie O, Gros J Timed Collinear Activation of Hox Genes during Gastrulation Controls the Avian Forelimb Position. Curr Biol. 2019 Jan 7;29(1):35-50.e4. Kinoshita et al. Apical Accumulation of Rho in the Neural Plate Is Important for Neural Plate Cell Shape Change and Neural Tube Formation Molecular Biology of the Cell, Volume 19, Issue 5, Pages 2289-2299, May 2008 Iimura et al. Dual mode of paraxial mesoderm formation during chick gastrulation PNAS, Volume 104, Number 8, Pages 2744-2749, 20 February 2007 Tadahiro Iimura and Olivier Pourquie Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression Nature, Volume 442, Number 7102, Pages 568-571, 3 August 2006 Wakamatsu et al. Multiple roles of Sox2, an HMG-box transcription factor in avian neural crest development Developmental Dynamics, Volume 229, Issue 1, Pages 74-86, January 2004 Catherine E. Krull A primer on using in ovo electroporation to analyze gene function Developmental Dynamics, Volume 229, Issue 3, Pages 433-439, March 2004 Toyofuku et al. Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2 Genes & Development, Volume 18, Issue 4, Pages 435-447, 15 February 2004 Endo et al. Bimodal functions of Notch-mediated signaling are involved in neural crest formation during avian ectoderm development Development, Volume 129, Issue 4, Pages 863-873, February 2002 Kobayashi et al. Early subdivisions in the neural plate define distinct competence for inductive signals Development, Volume 129, Issue 1, Pages 83-93, January 2002

Chicken Retina ex vivo	Estie Schick, Sean D McCaffery, Erin E Keblish, Cassandra Thakurdin, Mark M Emerson Lineage Tracing Analysis of Cone Photoreceptor Associated Cis-Regulatory Elements in the Developing Chicken Retina Sci Rep, 9 (1), 9358 2019 Jun 27

Chicken Brain in vivo	Yamaguchi et al. In-vivo gene transfer into newly hatched chick brain by electroporation Neuroreport, Volume 18, Issue 8, Pages 735-739, 28 May 2007

Zebrafish Brain in vivo	Zou M, De Koninck P, Neve RL, Friedrich RW Fast gene transfer into the adult zebrafish brain by herpes simplex virus 1 (HSV-1) and electroporation: methods and optogenetic applications. Front Neural Circuits. 2014 May 6;8:41.

Zebrafish Retina in vivo/ex vivo	Kustermann et al. Survival, excitability, and transfection of retinal neurons in an organotypic culture of mature zebrafish retina Cell and Tissue Research, Volume 332, Number 2, Pages 195-209, May 2008 Thummel et al. Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina Developmental Neurobiology, Volume 68, Issue 3, Pages 392-408, 15 February 2008

Zebrafish Muscle in vivo	Masaki Iwaizumi, Hayato Yokoi, Tohru Suzuki Plasmid Delivery by Electroporation Into Fish Skeletal Muscle for Recombinant Protein Secretion and Uptake by Oocytes Fish Physiol Biochem. 2020 Feb 23.

Zebrafish Fin in vivo	Christen B, Robles V, Raya M, Paramonov I, Izpisúa Belmonte JC. Regeneration and reprogramming compared. BMC Biol. 2010 Jan 20;8:5. Thummel et al. Inhibition of zebrafish fin regeneration using in viv electroporation of morpholinos against fgfr1 and msxb Developmental Dynamics, Volume 235, Issu 2, Pages 336-346, February 2006 Bai et al. Matrix metalloproteinase expression and function during fin regeneration in zebrafish: Analysis of MT1-MMP, MMP2 and TIMP2B Matrix Biology, Volume 24, Issue 4, Pages 247-260, June 2005

Zebrafish Flounder Embryos	Ling Wang, Xungang Tan, Zhihao Wu, Lijuan Wang, Shuang Jiao, Yuxia Zou, Guanglei Ji, Feng You Targeted mutagenesis in the olive flounder (Paralichthys olivaceus) using the CRISPR/Cas9 system with electroporation Biologia volume 76, pages1297–1304 (2021) Changqing Zhang, Ziheng Ren, Zhiyuan Gong Transgenic Expression and Genome Editing by Electroporation of Zebrafish Embryos Mar Biotechnol (NY). 2020 Aug 4 Holmes KE, Wyatt MJ, Shen YC, Thompson DA, Barald KF. Direct delivery of MIF morpholinos into the zebrafish otocyst by injection and electroporation affects inner ear development. J Vis Exp. 2011 Jan 7;(47). pii: 2466.

Xenopus Blastema	Kazumasa Mitogawa, Aki Makanae, Akira Satoh Hyperinnervation improves Xenopus laevis limb regeneration Dev Biol. 2018 Jan 15;433(2):276-286.
Embryos	Chernet BT, Levin M. A Versatile Protocol for mRNA Electroporation of Xenopus laevis Embryos. Cold Spring Harb Protoc. 2012 Apr 1;2012(4). Sasagawa et al. Improved mRNA Electroporation Method for Xenopus Neurula Embryos Genesis, Volume 33, Issue 2, Pages 81-85, June 2002

Axolotls Blastema	Nicholas D Leigh, Sofia Sessa, Aline C Dragalzew, Duygu Payzin-Dogru, Josane F Sousa, Anthony N Aggouras, Kimberly Johnson, Garrett S Dunlap, Brian J Haas, Michael Levin, Igor Schneider, Jessica L Whited Von Willebrand Factor D and EGF Domains Is an Evolutionarily Conserved and Required Feature of Blastemas Capable of Multitissue Appendage Regeneration Evol Dev 2020 Mar 12 [Online ahead of print]
Limb or Dorsal root ganglion	Sakiya Yamamoto, Rena Kashimoto, Saya Furukawa, Hirotaka Sakamoto, Akira Satoh Comparing nerve-mediated FGF signalling in the early initiation phase of organ regeneration across mutliple amphibian species J Exp Zool B Mol Dev Evol. 2021 Nov;336(7):529-539.

Honey Bee Brain in vivo	Schulte C, Leboulle G, Otte M, Grünewald B, Gehne N, Beye Mo Honey bee promoter sequences for targeted gene expression. Insect Mol Biol. 2013 Aug;22(4):399-410. Takekazu Kunieda and Takeo Kubo In vivo gene transfer into the adult honeybee brain by using electroporation Biochemical and Biophysical Research Communications, Volume 318, Issue 1, Pages 25-31, 21 May 2004

Cricket Brain	Matsumoto CS et al. Targeted gene delivery in the cricket brain, using in vivo electroporation. J Insect Physiol. 2013 Dec;59(12):1235-41.

Cricket Embryos	Shinmyo et al. piggyBac-mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus Development, Growth & Differentiation, Volume 46, Issue 4, Pages 343-349, August 2004

Water Flea Water fleas in vivo	Kato Y et al. Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation. Ecotoxicology. 2010 Mar;19(3):589-92.

Planarian Planarians in vivo	Cristina González‐Estévez Chapter Twenty‐Seven Autophagy in Freshwater Planarians Methods in Enzymology, Volume 451, 2008, Pages 439-465 Gonzalez-Estevez et al. Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker PNAS, Volume 100, Number 24, Pages 14046-14051, 25 November 2003

Butterfly Larvae in vivo	H Jin, T Seki, J Yamaguchi, H Fujiwara Prepatterning of Papilio xuthus caterpillar camouflage is controlled by three homeobox genes: clawless, abdominal-A, and Abdominal-B Sci Adv. 2019 Apr 10;5(4):eaav7569.

Exosomes	Kosuke Noguchi, Mami Hirano, Takuya Hashimoto, Eiji Yuba, Tomoka Takatani-Nakase, Ikuhiko Nakase Effects of Lyophilization of Arginine-rich Cell-penetrating Peptide-modified Extracellular Vesicles on Intracellular Delivery. Anticancer Res, 39 (12), 6701-6709 Dec 2019 Ikuhiko Nakase, Kosuke Noguchi, Ayako Aoki, Tomoka Takatani-Nakase, Ikuo Fujii, Shiroh Futaki Arginine-rich Cell-Penetrating Peptide-Modified Extracellular Vesicles for Active Macropinocytosis Induction and Efficient Intracellular Delivery. Sci Rep, 7 (1), 1991 2017 May 16 Tomoya Takenaka, Shinya Nakai, Miku Katayama, Mami Hirano, Natsumi Ueno, Kosuke Noguchi, Tomoka Takatani-Nakase, Ikuo Fujii, Susumu S Kobayashi, Ikuhiko Nakase Effects of Gefitinib Treatment on Cellular Uptake of Extracellular Vesicles in EGFR-mutant Non-Small Cell Lung Cancer Cells. Int J Pharm, 572, 118762 2019 Dec 15 Ikuhiko Nakase, Kosuke Noguchi, Ikuo Fujii, Shiroh Futaki Vectorization of Biomacromolecules Into Cells Using Extracellular Vesicles With Enhanced Internalization Induced by Macropinocytosis. Sci Rep, 6, 34937 2016 Oct 17 Nakase I, Kobayashi NB, Takatani-Nakase T, Yoshida T Active macropinocytosis induction by stimulation of epidermal growth factor receptor and oncogenic Ras expression potentiates cellular uptake efficacy of exosomes. Sci Rep. 2015 Jun 3;5:10300. Nakase I, Futaki S Combined treatment with a pH-sensitive fusogenic peptide and cationic lipids achieves enhanced cytosolic delivery of exosomes. Sci Rep. 2015 May 26;5:10112.

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ｴﾚｸﾄﾛﾎﾟﾚｰｼｮﾝ NEPA21/ELEPO21

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ｴﾚｸﾄﾛﾎﾟﾚｰｼｮﾝ NEPA21/ELEPO21

電気式細胞融合 ECFG21/LF201/LF101

ソノポレーション ソノポール

ソノポレーション ソニトロン

リアルタイム細胞解析

エレクトロポレーション アプリケーション別文献

NEPA21遺伝子導入装置が用いられた文献 （下位機種CUY21遺伝子導入装置も含む）

ソノポレーションソノポール

ソノポレーションソニトロン

エレクトロポレーションアプリケーション別文献

NEPA21遺伝子導入装置が用いられた文献（下位機種CUY21遺伝子導入装置も含む）