PF-NP-200

Liquid nitrogen, alcohol and cryogen free: Reduced contamination risks!

Compatible with cryovials, blood/cell culture bags and straws

Custom design of the block is available (e.g., 73X cryovials and 96X 1 mL tubes)

• Compact and portable
• Stand-alone and touch screen operation device without PC
• Temperature log can be exported from the freezer in operation via USB stick
• Compatible with CPC, GMP and isolator environment
• Freezing block heater: Quick temperature reset of the block for the next freeze cycle
• Power failure restart function for a quick recovery from a unpredictable power failure
• Optional power source from an accessory socket in car is available

• IQ/OQ, GMP, GLP and FDA 21 CFR part 11 compliant

• Stem cells (ESCs, iPSCs, MSCs and More)
• Primary cells and cell lines (Mammalian)
• Cord blood
• Embryos (Human, Mouse, Fish and More)
• Sperm (Human, Horse, Bird, Fish and More)
• Tissue (Biopsy sample, Mouse, Fish and More)

Fig 1 and 2: Temperature log of the 2mL cryovials and 25 mL cell culture bags
The cryovial and cell bags are freezed almost accordingly to the freezing block. A couple of cell culture bags were stacked, and the freezing results of both bags on top and bottom were almost the same.

Fig 3 and 4: Viability result of cells after freezing
The cells are diluted according to the protocol of a cell freezing media and filled into the cryovials and cell culture bags. The cells are then freezed by the PF-NP-200 at -1℃/min.
With cryovial, an excellent viability result was achieved among all the cell types.
The viability results of the both cell cluture bags were as good as the results with cryovials.

*Fig 2 and 4: Collaboration with Excellent Young Researcher (LEADER) Shinya Yamahira, St. Luke's International Univrsity, Center for Medical Sciences