*The NEPA21 electroporator can cover all
application range of the second-class CUY21 electroporators.
*This
instrument complies with CE marking.
Request a Demo |
See the performance of the NEPA21 electroporator with your cells. |
The novel 4-step pulse with voltage decay results in higher transfection efficiency and higher viability WITHOUT special buffers.
1) Poring Pulse Mode:
higher voltage,
shorter duration, multiple pulses, voltage decay
This poring pulse is for forming pores
(small holes) in cell membrane with minimum damage.
2) Polarity Exchanged Poring Pulse
This can be
applied to tissue transfection as well.
3) Transfer Pulse Mode:
lower voltage, longer duration, multiple pulses, voltage dcay.
This transfer pulse is for
delivering the target molecules (DNA, RNA, etc.) into cells with minimum
damage.
4) Polarity Exchanged Transfer Pulse
This can increase the transfection efficiency.
It is possible to achieve high transfection efficiency and high viability without resourse to special buffers for difficult-to-transfect cells such as primary cells, stem cells, immune cells, blood cells, etc.
*Photo: CU500 Cuvette Chamber, CU600 Cuvette Holder, EC-002 Cuvettes and NEPA21 Electroporator
Below are the data of NEPA21 cuvette transfection with high efficiency and high viability.
Transfection into Primary Cells
BMMC Primary Mouse Bone Marrow-Derived Mast cells | |
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Viability: 80% | Transfection Efficiency: 83% |
FACS Data |
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Transfection into Stem Cells: ESCs, iPSCs and more
Human iPS Cells | Human ES Cells |
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3 days after electroporation | Stable expression |
Mouse Neurospheres | |
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Viability: 90% | Transfection Efficiency: 75% |
Embryoid Body in Adherence from human iPS Cells | |
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Generation of Induced Pluripotent Stem Cells (iPSCs)
Data of disease-specific iPSC Generation (disease: LQT) | |
- transfection of multiple episomal plamids into B cells | |
The picture below shows an iPSC colony one month after electroporation. (5X objective) | |
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The pictures below show Alkaline phosphatase stain. (left:4X objective, right:10X objective) | |
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The pictures below show good expressions of stem cell markers TRA1-60 and OCT4. (left green:TRA1-60, right green:OCT4, blue:DAPI, red:Phalloidin) | |
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Data provided courtesy of Dr. Toshio Nakanishi laboratory, Department of Pediatric Cardiology, Tokyo Women's Medical University, Japan
Transfection into Cell Lines
293T (HEK293T): Human Embryonic Kidney Cells | |
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Viability: 83% | Transfection Efficiency: 87% |
Jurkat: Human T-cell Leukemia Cells | |
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Viability: 90% | Transfection Efficiency: 85% |
Comparison with Competitors
No Special Buffers for the NEPA21!!
Transfection Device |
NEPA21 (Nepa Gene) |
Competitor N (Company L) |
Competitor N (Company I (L)) |
Characteristics | New Electroporation No Special Buffers |
Electroporation and Special Buffers |
Electroporation and Special Tips |
Transfection Efficiency |
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Cell Viability | ![]() |
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Consumables (Disposable Kits) |
Economy Cuvettes USD 2.00/reaction |
Special Kits USD 20.00/reaction |
Special Kits USD 20.00/reaction |
Is your lab still using a transfection device that requires expensive disposable kits?
We hear from a lot of researchers that they are not satisfied with its high running cost.
The running cost of NEPA21 is much lower than other transfection devices!
By using the cell-culture-plalte Electrode CUY900 series, it is now possible to transfer DNA/RNA directly into cells IN ADHERENCE in a commercially available multi-well plate.
Transfection into Primary Neurons
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pCAGGS-EGFP plasmid was transferred into primary
neurons cultured for 6 days in adherent state.
The neurons were
prepared from E15 mouse cerebral cortex.
A): | 2 steps pulse electroporation using the electrodes (CUY900-13-3-5) for adherent cells |
B): | EGFP fluorescence image of the neurons 2 days after electroporation |
C): | High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency. |
D): | High magnification image of Figure C (x40). Neurites are shown clearly. |
Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan |
The NEPA21 electroporator and the CUY series in-vivo electrodes make it possible to transfer DNA/RNA into in vivo mice/rats.
Transfection into In Vivo Muscle
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pCAGGS-lacZ that expresses beta-galactosidase was transfected into muscle via Electroporation. Five days after the DNA transfer, the expression of the lacZ gene was visualized by X-gal staining for beta-galactosidase activity. The expression was observed in a lot of muscle fibers. Meanwhile there was no expression in control samples without electroporation. | ||
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Whole muscle with electroporation |
Whole muscle without electroporation |
Transverse section with electroporation |
Transverse section without electroporation |
Transfection into In Vivo Brain, Retina, Skin, Testis, Liver, Kidney and More
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Mouse Brain | Mouse Skin | Mouse Testes | Rat Retina | Honeybee Brain | Silkworm Eggs |
NEPA21 electroporator and the CUY series in-utero electrodes make it possible to transfer DNA/RNA into mouse/rat embryos (cerebral cortex, hippocampus, spinal cord, and more.)
Transfection into Mouse/Rat Embryonic Brains
pCAG-EGFP was injected into the both lateral ventricles of E14.5 mouse embryos and electronic pulses (33V, 50msec) were charged four times. 3 days later, the embryos (E17.5) were fixed and the brains were removed and examined under a fluorescence stereomicroscope (Fig. A). Fluorescence was observed in the lateral region of the hemisphere onto which the anode had been placed and in the medial region of the opposite hemisphere. And brains were frozen and sliced and the fluorescent image was obtained with a confocal laser microscope (Fig. B).
Transfection into Chick Embryos
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Transfection into the central canal | Transfection into the somites (A, E), hematopoietic system (B, F), notochord (C, G), and lateral plate mesoderm (D, H) |
Transfection into Brain Slices
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![]() The neurons were prepared from P7 rat hippocampus. Electroporation was performed after 11 DIV. |
Transfection into Islets of Langerhans
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Super Electroporator NEPA21 | |||
Dimensions | 346(W) X 330(D) X 113(H) mm | Weight | 7.5 kg |
*All features and specifications subject to change without notice.