Products > Transfection/Electroporation > NEPA21 Super Electroporator

NEPA21 Super Electroporator

High Transfection Efficiency & High Viability WITHOUT Special Buffers

NEPA21

NEPA21 Applications

In Vitro Transfection

into difficult-to-transfect cells such as primary cells, stem cells, etc.
into any cell lines
with electroporation cuvettes
with cell-culture-plate electrodes for cells in adherence

In Vivo Transfection

into mouse/rat brain, eye, muscle, skin, liver, kidney, testis, etc.
with in vivo electrodes

In Utero Transfection

into mouse/rat embryos: cerebral cortex, hippocampus, spinal cord, etc.
with in utero electrodes

In Ovo Transfection

into chick embryos: neural tube, limb bud, digestive organ, etc.
with in ovo electrodes

Ex-Vivo Transfection

into explants, brain slices, organs, whole embryos, etc.
with ex vivo electrodes

*The NEPA21 electroporator can cover all application range of the second-class CUY21 electroporators.
*This instrument complies with CE marking.

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See the performance of the NEPA21 electroporator with your cells.

NEPA21 Novel 4-Step Multiple Electroporation Pulse

multipulses

The novel 4-step pulse with voltage decay results in higher transfection efficiency and higher viability WITHOUT special buffers.

1) Poring Pulse Mode:
higher voltage, shorter duration, multiple pulses, voltage decay
This poring pulse is for forming pores (small holes) in cell membrane with minimum damage.

2) Polarity Exchanged Poring Pulse
This can be applied to tissue transfection as well.

3) Transfer Pulse Mode:
lower voltage, longer duration, multiple pulses, voltage dcay.
This transfer pulse is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum damage.

4) Polarity Exchanged Transfer Pulse
This can increase the transfection efficiency.

NEPA21 Cuvette Electroporation

NEPA21 and cuvettes

It is possible to achieve high transfection efficiency and high viability without resourse to special buffers for difficult-to-transfect cells such as primary cells, stem cells, immune cells, blood cells, etc.

*Photo: CU500 Cuvette Chamber, CU600 Cuvette Holder, EC-002 Cuvettes and NEPA21 Electroporator

Below are the data of NEPA21 cuvette transfection with high efficiency and high viability.

Transfection into Primary Cells

BMMC Primary Mouse Bone Marrow-Derived Mast cells

Viability: 80%	Transfection Efficiency: 83%

FACS Data

See MORE.
See the Publications.

Transfection into Stem Cells: ESCs, iPSCs and more

Human iPS Cells	Human ES Cells

3 days after electroporation	Stable expression

Mouse Neurospheres

Viability: 90%	Transfection Efficiency: 75%

Embryoid Body in Adherence from human iPS Cells

See MORE.
See the Publications.

Generation of Induced Pluripotent Stem Cells (iPSCs)

Data of disease-specific iPSC Generation (disease: LQT)
- transfection of multiple episomal plamids into B cells
The picture below shows an iPSC colony one month after electroporation. (5X objective)

The pictures below show Alkaline phosphatase stain. (left:4X objective, right:10X objective)

The pictures below show good expressions of stem cell markers TRA1-60 and OCT4. (left green:TRA1-60, right green:OCT4, blue:DAPI, red:Phalloidin)

Data provided courtesy of Dr. Toshio Nakanishi laboratory, Department of Pediatric Cardiology, Tokyo Women's Medical University, Japan

Transfection into Cell Lines

293T (HEK293T): Human Embryonic Kidney Cells

Viability: 83%	Transfection Efficiency: 87%

Jurkat: Human T-cell Leukemia Cells

Viability: 90%	Transfection Efficiency: 85%

See MORE.
See the Publications.

Comparison with Competitors

No Special Buffers for the NEPA21!!

Transfection Device	NEPA21 (Nepa Gene)	Competitor N (Company L)	Competitor N (Company I (L))
Characteristics	New Electroporation No Special Buffers	Electroporation and Special Buffers	Electroporation and Special Tips
Transfection Efficiency
Cell Viability
Consumables (Disposable Kits)	Economy Cuvettes USD 2.00/reaction	Special Kits USD 20.00/reaction	Special Kits USD 20.00/reaction

Is your lab still using a transfection device that requires expensive disposable kits?
We hear from a lot of researchers that they are not satisfied with its high running cost.
The running cost of NEPA21 is much lower than other transfection devices!

See the Nepa Electroporation Cuvettes.

NEPA21 Cell-Culture-Plate Electroporation

By using the cell-culture-plalte Electrode CUY900 series, it is now possible to transfer DNA/RNA directly into cells IN ADHERENCE in a commercially available multi-well plate.

Transfection into Primary Neurons

	Click the photo to see the enlarged one.
Click the photo to see the enlarged one.	Click the photo to see the enlarged one.

pCAGGS-EGFP plasmid was transferred into primary neurons cultured for 6 days in adherent state.
The neurons were prepared from E15 mouse cerebral cortex.

A):	2 steps pulse electroporation using the electrodes (CUY900-13-3-5) for adherent cells
B):	EGFP fluorescence image of the neurons 2 days after electroporation
C):	High magnification image of Figure B. Many robust EGFP signals suggest high transfection efficiency.
D):	High magnification image of Figure C (x40). Neurites are shown clearly.
Data provided courtesy of Department of Neurochemistry, National Institute of Neuroscience, Japan

See MORE.
See the Electrodes.
See the Publications.

NEPA21 In Vivo Electroporation

The NEPA21 electroporator and the CUY series in-vivo electrodes make it possible to transfer DNA/RNA into in vivo mice/rats.

Transfection into In Vivo Muscle

	pCAGGS-lacZ that expresses beta-galactosidase was transfected into muscle via Electroporation. Five days after the DNA transfer, the expression of the lacZ gene was visualized by X-gal staining for beta-galactosidase activity. The expression was observed in a lot of muscle fibers. Meanwhile there was no expression in control samples without electroporation.

Whole muscle with electroporation	Whole muscle without electroporation	Transverse section with electroporation	Transverse section without electroporation

Transfection into In Vivo Brain, Retina, Skin, Testis, Liver, Kidney and More


Mouse Brain	Mouse Skin	Mouse Testes	Rat Retina	Honeybee Brain	Silkworm Eggs

See MORE.
See the Electrodes.
See the Publications.

NEPA21 In Utero Electroporation

NEPA21 electroporator and the CUY series in-utero electrodes make it possible to transfer DNA/RNA into mouse/rat embryos (cerebral cortex, hippocampus, spinal cord, and more.)

Transfection into Mouse/Rat Embryonic Brains

pCAG-EGFP was injected into the both lateral ventricles of E14.5 mouse embryos and electronic pulses (33V, 50msec) were charged four times. 3 days later, the embryos (E17.5) were fixed and the brains were removed and examined under a fluorescence stereomicroscope (Fig. A). Fluorescence was observed in the lateral region of the hemisphere onto which the anode had been placed and in the medial region of the opposite hemisphere. And brains were frozen and sliced and the fluorescent image was obtained with a confocal laser microscope (Fig. B).

See MORE.
See the Electrodes.
See the Publications.

NEPA21 In Ovo Electroporation

Transfection into Chick Embryos


Transfection into the central canal	Transfection into the somites (A, E), hematopoietic system (B, F), notochord (C, G), and lateral plate mesoderm (D, H)